Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Confirmation of Trichloroethylene-Degrading Enzyme from a Phenol-Degrading Bacterium, Pseudomonas sp. EL-04J

페놀분해세균인 Pseudomonas sp. EL-04J로부터 Trichloroethylene 분해효소의 확인

  • Park, Geun-Tae (Department of Microbiology, Pusan National University) ;
  • Kim, Ho-Sung (Department of Microbiology, Pusan National University) ;
  • Son, Hong-Ju (Department of Biotechnology, Miryang National University) ;
  • Lee, Gun (Department of Microbiology, Pusan National University) ;
  • Park, Sung-Hoon (Division of Chemical Engineering, and Institute for Environmental Technology) ;
  • Lee, Sang-Jun (Department of Microbiology, Pusan National University)
  • 박근태 (부산대학교 미생물학과) ;
  • 김호성 (부산대학교 미생물학과) ;
  • 손홍주 (밀양대학교 생물공학과) ;
  • 이건 (부산대학교 미생물학과) ;
  • 박성훈 (부산대학교 응용화학공학부 및 환경기술산업개발연구소) ;
  • 이상준 (부산대학교 미생물학과)
  • Published : 2002.10.01
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Abstract

Pseudomonas sp. EL-041 was previously isolated from phenol-acclimated activated sludge. This bacterium was capable of degrading phenol and cometabolizing trichloroethylene (TCE). In this study, we report the identification of trichloroethylene- degrading enzyme in Pseudomonas sp. EL-041 by the investigation of enzyme activity and DNA sequencing of specific phenol oxygenase gene. As the results of experiment, trichloroethylene-degrading enzyme in Pseudomonas sp. EL-041 was monooxygenase and suspected to phenol hydroxylase.

Pseudomonas sp. EL-041는 페놀로 적응된 활성오니로부터 분리되었으며, 페놀을 분해할 수 있는 동시에 trick-loroethylene(TCE)을 공동대사할 수 있는 세균이다. 본 연구에서는 Pseudomonas sp. EL-041에서 발현되는 trick-loroethylene 분해효소의 종류를 효소활성 측정 및 specific phenol oxygenase gene의 DNA sequencing을 통하여 조사하였으며, 그 결과 본 균주의 trichloroethylene 분해효소는 monooxygenase였으며, phenol hydroxylase로 추정되었다.

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References

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