Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Detection of Escherichia coli O157:H7 Using Immunosensor Based on Surface Plasmon Resonance

  • Oh, Byung-Keun (Department of Chemical Engineering, Sogang University) ;
  • Kim, Young-Kee (Department of Chemical Engineering, Hankyong National University) ;
  • Bae, Young-Min (Department of Chemical Engineering, Sogang University) ;
  • Lee, Won-Hong (Department of Chemical Engineering, Sogang University) ;
  • Choi, Jeong-Woo (Department of Chemical Engineering, Sogang University)
  • Published : 2002.10.01
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Abstract

An immunosensor based on surface plasmon resonance (SPR) with a self-assembled protein G layer was developed for the detection of Escherichia coli O157:H7. A self-assembled protein C layer on a gold (Au) surface was fabricated by adsorbing the mixture of 11-mercaptoundecanoic acid (MUA) and hexanethiol at various molar ratios and by activating chemical binding between free amine (-$NH_2$) of protein G and 11-(MUA) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC) in series. The formation of a self-assembled protein G layer on an Au substrate and the binding of the antibody and antigen in series were confirmed by SPR spectroscopy. The surface morphology analyses of the self-assembled protein G layer on the Au substrate, monoclonal antibody (Mab) against E. coli O157:H7 which was immobilized on protein G, and bound E. coli O157:H7 extracts on Immobilized Mab against E. coii O157:H7 were performed by atomic force microscopy (AFM). The detection limit of the SPR-based immunosensor for E. coli O157:H7 was found to be about $10^4$ cells/ml.

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References

  1. Biotechnol. v.5 Bacterial Fc receptors Boyle, M. D. P.;K. J. Reis. https://doi.org/10.1038/nbt0787-697
  2. J. Microbiol. Biotechnol. v.11 Fluorescence immunoassay of HDL and LDL using protein A LB film. Choi, J.-W.;J. H. Park;W. Lee;B.-K. Oh;J. Min;W. H. Lee.
  3. Biosens. Bioelectron. v.13 Covalent coupling of immunoglobulin G to self-assembled monolayers as a method for immobilizing the interfacial recognition layer of a surface plasmon resonance immunosensor. Darren, M. D.;C. C. David;Y. Hong-Xing;R. L. Christopher. https://doi.org/10.1016/S0956-5663(98)00059-1
  4. Infection v.17 ELISA for antibodies to lipid A, lipopolysaccarides and other hydrophobic antigens. Freudenberg, M.;A. Fomsgaard;I. Mitov;C. Galanos. https://doi.org/10.1007/BF01650719
  5. Z. Phys. v.241 Die bestimmung optischer konstanten von metallen durch anregung von oberflachenplasmaschwingungen. Kretschmann, E. https://doi.org/10.1007/BF01395428
  6. Appl. Environ. Microbiol. v.64 Comparison of the BAX for screening/E. coli O157:H7 method with conventional methods for detection of extremely low levels of Escherichia coli O157:H7 in ground beef. Johnson, J. L.;C. L. Brooke;S. J. Fritschel.
  7. Soil Use Manage. v.15 Potential health risks associated with the persistence of E. coli O157 in agricultural environments Jones, D. L. https://doi.org/10.1111/j.1475-2743.1999.tb00069.x
  8. J. Food Sci. Agric. v.79 Use of chicken egg-yolk antibodies against K88+fimbral antigen for quantitative analysis of enterotoxigenic Escherichia coli(ETEC) K88 by a sandwich ELISA. Kim, J. W.;L. Z. Jin;S. H. Cho;R. R. Marquardat;A. A. Frohlich;S. K. Baidoo. https://doi.org/10.1002/(SICI)1097-0010(199908)79:11<1513::AID-JSFA396>3.0.CO;2-0
  9. Lancet v.349 Escherichia coli O157: outbreak in central Scotland. Liddell, K. G.
  10. Langmuir v.14 Two-dimensional structure of disulfides and thiols on gold(111). Nelles, G.;H. Schonherr;M. Jaschke;H. Wolf;M. Schaub;I. Kuther;W. Tremel;E. Bamberg;H. Ringsdorf;H. Butt. https://doi.org/10.1021/la9709709
  11. J. Clin. Microbiol. v.29 Production and characterization of a monoclonal antibody specific for enterohaemorrhagic Escherichia coli O157:H7 and O26:H11. Padhye, N. V.;M. P. Doyle.
  12. Sensor Actuat. B v.49 A surface plasmon resonance-based immunosensor for highly sensitive detection of morphine. Sakai, G.;K. Ogata;T. Uda;N. Miura;N. Yamazoe. https://doi.org/10.1016/S0925-4005(98)00107-5
  13. Biochem. Biophys. Acta v.1331 Surface plasmon resonance spectroscopy as a tool for investigating the biochemical and biophysical properties of membrane protein systmes. Ⅱ: Applications to biological systems. Salmon, Z.;H. A. Macleod;G. Tollin. https://doi.org/10.1016/S0304-4157(97)00003-8
  14. Sensor Actuat. B v.52 Surface design SPR-based immunosensor for the effective binding of antigen or antibody in the evanescent field using mixed polymer matrix. Toyama, S.;A. Shoji;Y. Yoshida;S. Yamauchi;Y. Ikariyama. https://doi.org/10.1016/S0925-4005(98)00257-3
  15. Appl. Microbiol. v.19 Verocytotoxin-producing Escherichia coli O157 in beefburgers linked to an outbreak of diarrhea, haemorrhagic colitis and haemolytic uraemic syndrome in Britain. Willshaw, G. A.;J. Thirlwell;A. P. Jones;S. Parry;R. L. Salmon;M. Hickey. https://doi.org/10.1111/j.1472-765X.1994.tb00461.x
  16. Colloid Surface B v.21 Labelling and binding od poly(L-lysine) to functionalized gold surface. Combined FT-IRRAS and XPS characterization. Yam, C. M.;L. Zheng;M. Salmain;C. M. Pradier;P. Marcus;G. Jaouen. https://doi.org/10.1016/S0927-7765(00)00215-0