Journal of Microbiology and Biotechnology

  • 제12권5호
  • /
  • Pages.746-752
  • /
  • 2002
  • /
  • 1017-7825(pISSN)
  • /
  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Effect of Enterococcus faecalis strain PL9003 on Adherence and Growth of Helicobacter pylori

  • Nam, Hye-Ran (Department of Biology, Seoul Women's University) ;
  • Ha, Mi-Sun (Department of Biology, Seoul Women's University) ;
  • Lee, En-Jung (Department of Biology, Seoul Women's University) ;
  • Lee, Yeon-Hee (Department of Biology, Seoul Women's University)
  • 발행 : 2002.10.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

The purpose of the present study was to examine the antagonistic activities of Enterococcus faecalis strain PL9003 (PL9003) on Helicobacter pylori. This strain was isolated from infant feces and found to inhibit both the growth of H. pylori and its in vitro adherence to the human gastric cell line MKN-45. The binding of PL9003 to MKN-45 was observed under a light microscope after Cram staining and under a scanning electron microscope. When detected with an FITC-conjugate antibody, both viable and nonviable PL9003 were found to decrease the number of H. pylori bound to MKN-45. When detected by an enzyme-linked immunoabsorbent assay, about 70% of the H. pylori bound on MKN-45 disappeared with the four-1314 addition of viable or nonviable PL9003. The spent culture supernatant (SCS) of PL9003 also decreased the viability of H pylori even after neutralization and pepsin treatment. The above results suggest that PL9003 has a potential as a new probiotic for the stomach.

키워드

참고문헌

  1. Amer. J. Gastroenterol. v.93 Lactic acid-mediated suppression of Helicobacter pylori by the oral administration of Lactobacillus salivarius as a probiotic in a gnotobiotic murine model Aiba, Y.;N. Suzuki;A. M. Kabir;A. Takagi;Y. Koga https://doi.org/10.1111/j.1572-0241.1998.00600.x
  2. J. Microbiol. Biotechnol. v.10 Anti-Helicobacter pylori activity of Bifidobacterium spp. Bae, E.;D. Kim;M. J. Han
  3. Gut v.35 Lactobacillus acidophilus strain LA1 binds to cultured human intestinal cell lines and inhibits cell-attachment and cell-invasion by enterovirulent Bernet-Camard, M. F.;D. Brassart;J. R. Neeser;A. L. Servin https://doi.org/10.1136/gut.35.4.483
  4. Appl. Environ. Microbiol. v.63 The human Lactobacillus acidophilus strain LA1 secretes a nonbacteriocin antibacterial substance(s) active in vitro and in vivo Bernet-Camard, M. F.;V. Lievin;D. Brassart;J. R. Neeser;A. L. Servin;S. Hudault
  5. Science v.262 Attachment of Helicobacter pylori to human gastric epithelium mediated by blood group antigens Boren, T.;P. Falk;K. A. Roth;G. Larson;S. Normark https://doi.org/10.1126/science.8018146
  6. Methods in Practical Laboratory Bacteriology The use of enzyme-linked immunosorbent assay in bacteriology Chart, H.
  7. FEMS Immunol. Med. Microbiol. v.16 Cell envelope characteristics of Helicobacter pylori: Their role in adherence to mucosal surfaces and virulence Clyne, M.;B. Drumm https://doi.org/10.1111/j.1574-695X.1996.tb00130.x
  8. Antimicrob. Agents Chemother. v.41 Antibacterial effect of the adhering human Lactobacillus acidophilus strain LB. Coconnier, M. H.;V. Lievin;M. F. Bernet-Camard;S. Hudault;A. L. Servin.
  9. Appl. Environ. Microbiol. v.64 Antagonistic activity against Helicobacter infection in vitro and in vitro by the human Lactobacillus acidophilus strain LB Coconnier, M. H.;V. Lievin;F. Hemery;A. L. Servin
  10. J. Gen. Microbiol. v.135 Protein-mediated adhesion of Lactobacillus fermentum strain 737 to mouse stomach squamous epithelium Conway, P. L.;S. Kjelleberg.
  11. ASM News v.61 Helicobacter pylori: A bacterial cause of gastritis, peptic ulcer disease, and gastric cancer Cover, T. L.;M. J. Blaser
  12. Helicobacter pylori. Clin. Microbiol. Rev. v.10 Dunn, B. E.;H. Cohen;M. J. Blaser
  13. J. Microbiol. Biotechnol. v.11 Enzyme-linked, biotin-streptavidin bacterial-adhesion assay for Helicobacter pyroli lectin-like interactions with cultured cells. Guzman-Mruillo, M.;F. Ascencio.
  14. Bergey's Manual of Systematic Bacteriology. v.2 Anaerobic streptococci Hardie, J. M.;p. H. A. Sneath(ed.);N. S. Mair(ed.);M. E. Sharpe(ed.);J. G. Holt(ed.)
  15. J. Gen. Microbiol. v.138 Adhesion to porcine squamous epithelium of saccharide and protein moieties of Lactobacillus fermentum strain 104-S. Henriksson, A.;P. L. Conway. https://doi.org/10.1099/00221287-138-12-2657
  16. Mol. Chem. Neuropath. v.34 Determination of ammonia in cerebrospinal fluid using the indophenol direct method. Huizenga, J. R.;A. W. Teelken;A. Tangerman;A. E. de Jager;C. H. Gips;P. L. Jansen https://doi.org/10.1007/BF02815078
  17. Microbiol. Rev. v.59 Bacteriocins of gram-positive bacteria Jack, R. W.;J. R. Tagg;B. Ray.
  18. Gut v.41 Prevention of Helicobacter pylori infection by lactobacilli in a gnotobiotic murine model. Kabir, A. M.;Y. Aiba;A. Takagi;S. Kamiya;T. Miwa;Y. Koga. https://doi.org/10.1136/gut.41.1.49
  19. Amer. J. Clin. Nutr. v.30 Specific adhesion of lactobacilli to keratinized epithelial cells of the rat stomach in vitro. Kawai, Y.;N. Suegara.
  20. J. Microbiol. Biotechnol. v.9 Identification and characterizaton of bacteriocin-producing lactic acid bacteria isolated from kimchi. Lee, H.;C. Park;Y. Joo;S. Kim;J. Yoon;Y. Park;I. Hwang;J. Ahn;T. I. Mheen
  21. J. Microbiol. Biotechnol. v.9 Lactobacillus acidophilus inhibits the Helicobacter pylori adherence Lee, Y.;E. Shin;J. Lee;J. H. Park.
  22. Digestion v.60 Effect of whey-based culture supernatant of Lactobacillus acidophilus (johnsonii) La1 on Helicobacter pylori infection in humans. Michetti, P.;G. Dorta;P. H. Wiesel;D. Brassart;E. Verdu;M. Herranz;C. Felley;N. Porta;M. Rouvet;A. L. Blum;I. Corthesy-Theulaz. https://doi.org/10.1159/000007660
  23. J. Appl. Bacteriol. v.79 In vitro Inhibition of Helicobacter pylori NCTC 11637 by organic acids and lactic acid bacteria. Mildolo, P. D.;J. R. Lambert;R. Hull;F. Luo;M. L. Grayson. https://doi.org/10.1111/j.1365-2672.1995.tb03164.x
  24. Antimicrob. Agents Chemother. v.45 In vitro anti-Helicobacter pylori activity of the probiotic atrain Bacillus subtilis 3 is due to secreton of antibiotics. Pinchuk, I. V.;P. Bressollier;B. Verneuil;B. Fenet;I. B. Sorokulova;F. Megraud;M. C. Urdaci. https://doi.org/10.1128/AAC.45.11.3156-3161.2001
  25. Appl. Environ. Microbiol. v.65 The scientific basis for probiotic strains of Lactobacillus. Reid, G.
  26. J. Nutr. v.130 no.2S Suppl The role of probiotic cultrues in the control of gastrointestinal health. Rolfe, R. D.
  27. J. Antimicrob. Chemother v.47 Suppressive effect of Lactobacillus gasseri OLL 2716 (LG21) on Helicobacter pylori infection in humans. Sakamoto, I.;M. Igarashi;K. Kimura;A. Takagi;T. Miwa;Y. Koga. https://doi.org/10.1093/jac/47.5.709
  28. Appl. Environ. Microbiol. v.52 Lipoteichoic acids in Lactobacillus strains that colonize the mouse gastric epithelium. Sherman, L. A.;D. C. Savage.
  29. J. Clin. Gastroenterol. v.14 no.Suppl. Mechanism of Helicobacter pylori pathogenesis: focus on mucus. Slomiany, B. L.;A. Slomiany. https://doi.org/10.1097/00004836-199206001-00020
  30. FEMS Microbiol. Rev. v.12 Lactic acid bacteria, their metabolic products and interference with microbial growth. Vandenbergh, P. A. https://doi.org/10.1111/j.1574-6976.1993.tb00020.x
  31. Appl. Environ. microbiol. v.66 Colonization of the stratified squamous epithelium of the nonsecreting area of horse stomach by Lactobacilli. Yuki, N.;T. Shimazaki;A. Kushiro;K. Watanabe;K. Uchida;T. Yuyama;M. Morotomi. https://doi.org/10.1128/AEM.66.11.5030-5034.2000