KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

The Growth Promotion Effect of Useful Enterobacteria Bifidobacterium aolescentis KCTC 3216 by Combination of Natural Products Bearing Antioxioative Capacity

장내 유용세근 Bifidobacterium adolescentis KCTC 3216의 성장을 촉진시키는 항산화 천연산물의 조합구성

  • Published : 2002.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

The growth of enterobacteria, Bifidobacteriurn adolescentis KCTC 3216 was promoted by natural products bearing antioxidative capacity and combined two, three and four kinds of them. B. adolescentis was showed a good growth by Sophorae flos, Phellodendri cofex, Mori cortex radicis, Aurantii nobilis pericarpium, Angelicae gigantis radix, alone, and two mixed combinations were composed of Paeonia japonica and Theae foiium, Epimedii herba and Angelicae gigantis radix, Paeonia japonica and epimedii herba, Atractylodis rhizoma alba and angelicae gigantis radix, and three mixed combinations were oraganized with Theae folium, Paeonia japonica and epimedii herba, Theae folium, Beiamcanda chinensis and Paeonia japonica, Theae foiium, Astragaii radix and Mori cortex radicis, and four mixed combinations were formed with Seiamcanda chinensis, Angelicae gigantis radix, Epimedii herba, Theae folium, and Angeiicae gigantis radix, Epimedii herba, Paeonia japonica, Theae folium, and Epimedii herba, Paeonia japonica, Sophorae flos, Theae folium. The best four mixed combination for the growth of B. adolescentis was mixture of Epimedii herba, Paeonia iaponica, Sophorae flow and Theae foiium, which promoted 2.6 times than that of control, and its antioxidative capacity was also 5.6 times higher, and the ratio of elimination of hydroxyl radical was more than 80% in each dilution rate. As these combinations of natural products will activate some parts of body, they may be applied to pharmaceuitcal applications, functional foods, antiaging tea, also expected to promote useful entero bacterial growth for fermentative beverage bearing multifunction.

장내 유용세균인 Eifidobacterium adolescentis KCTC 3216은 천연산물 향가지 단독으로서도 성장이 촉진되었으며, 두가지, 세가지 및 네가지의 조합에 의하여도 특징적으로 성장이 촉진되었다. 그리고 이 균주는 천연산물의 배합 중에서 한가지의 천연산물로서는 괴화, 황백, 상백피, 진피, 당귀 등이 좋은 효과를 보였고, 두가지 천연산물의 배합에서는 백작약와 녹차, 음양곽과 당귀, 백작약과 음양곽, 백출과 당귀 등의 조합이, 세가지의 천연산물 배합에서는 녹차와 백작약 및 음양곽, 녹차와사간 및 백작약, 녹차와 황기 및 상백피 등의 조합이, 그리고 4가지 천연산물 배합에서는 사간, 당귀, 음양곽 및 녹차의 조합, 당귀, 음양곽, 백작약 및 녹차의 조합, 음양곽, 백작약, 괴화 및 녹차 등의 조합에서 잘 성장함을 보였으며, 음앙곽, 백작약, 괴화 및 녹차의 조합이 대조군 보다 2.6배의 성장 촉진 효과를 나타내었고, AUC는 659.88로써 대조군의 AUC보다 5.6배 높은 것으로 나타났다. 또한 4종류의 조합 중 당귀, 음양곽, 백작약 및 녹차의 조합의 AUC가 665.55로써 강한 것으로 났으며, 이 조합의 성장촉진효과는 대조군보다 2.1배의 성장률을 보였고 대조군의 AUC(116.61)보다 5.7배 정도 항산화력이 강한 것으로 나타났다 또한 4가지 종류 조합의 총 phenol 량은 녹차 중의 증제차나 볶음차 둥의 비발효 차보다 30배 이상 높은 것으로 나타났으며, 반 발효차인 오룡차보다는 32∼35배, 발효차인 홍차보다는 55∼60 정도 높은 것으로 나타났다. 이것은 항산화력이 강한 천연산물들을 이용하여 free radical의 제거와 동시에 B. adulescentis의 성장 촉진 물질로 사용할 수 있으며, 생체의 기능을 향상시킬 수 있는 기능성 제제로의 응용 가능성을 보였다.

Keywords

References

  1. Lunec, J. (1995), Oxygen Radical Activity-Detection and Measurement in vivo, In Encyclopedia of Analytical Science, Vol. 6, Townshend, A. Ed., p3679, Academic Press, U.S
  2. Nakada, C. (1997), Talk for Free Radical, p56, Koudansya, Japan
  3. Yosikawa, M., W. Kawano, and I. Yano. (2000), All of reactive oxygen and Free Radical, p8, Marusen L.T.D., Japan
  4. Chung, H. Y., H. J. Kim, K. J. Jung, J. S. Yoon, M. A. Yoo, K. W. Kim, and B. P. Yu. (2000), The inflammatory process in aging, Clin. Gerontol. 10, 202-222
  5. Harman, D. (1978), Free radical theory of agingnutritional implications. Age, 1, 143-150 https://doi.org/10.1007/BF02432188
  6. Kim, H. J., K. W. Kim, B. P. Yu, and H. Y. Chung (2000), The Effect of Age on Cyclooxygenase-2 gene Expression : NF-kB Activation and IkBa Dgradation, Free Radical BioI. Med. 28, 683-692 https://doi.org/10.1016/S0891-5849(99)00274-9
  7. Kwon, H. J., M. J. Kang, H. J. Kim, J. S. Choi, K. J. Paik, and H. Y. Chung (2000), Inhibition of NF-KB by Methyl CWorogenate from Eriobotrya japonica, Mol. Cell, 10, 241-246
  8. Yu B. P. (1996), Aging and oxidative stress-modulation by dietary restriction. Free Rad. BioI. Med. 21, 651-668 https://doi.org/10.1016/0891-5849(96)00162-1
  9. Zoubi, H. (1998), Methods for not to lose against free radicals, p13, Noubunkyo, Japan
  10. Li, H. C., S. Yashiki, J. Sonoda, H. Lou, S. K. Ghosh, J. J. Byrnes, C. Lema, T. Fujiyoshi, M. Karasuyama, and S. Sonoda (2000), Green tea polyphenols induce apoptosis in vitro in peripheral blood T lymphocytes of adult T-cell leukemia patients. Jpn. J. Cancer Res. 91, 34-40 https://doi.org/10.1111/j.1349-7006.2000.tb00857.x
  11. Outa. S. (1987), Food and Antioxidant, p38, Syokuhinzairyokenkyukai, Japan
  12. Peter T. P. (1998), The skin's Antioxidant Systems. Dermatol. Nurs. 10, 401-406
  13. Serafini, M., A. Ghiselli, and A. Ferro-Luzzi (1994), Red wine, tea, and antioxidants. Lancet, 344, 626-629 https://doi.org/10.1016/S0140-6736(94)92017-6
  14. Kawagan, S. (1996), Protocol for control of body functional material in food, p15, Kakuen Press Center, Japan
  15. Kogukuchi, N.(1999), Protocol for free radical experiments, p45, Suiyoonsa, Japan
  16. Torizaki, K. (1995), Protocol for lipid peroxide and free radical, p145, Kakuen Press Center, Japan
  17. Gan, K. K. (1982), Zugai Zouyou Kanbou Syohou, p190. Yakukyosihosya, Japan
  18. Huh, J. (1999), Dongeuibogam, p21, Bobinmunwhasa, Korea
  19. Kawhudo, Y. (1989), Proof of Pulse-Bangyakhappyeon, p15, Namsandang, Korea
  20. Mori, Y. Z. (1986), KanbouSyohounoKouseitoTekiyou, p20, Hakubustusyokan, Japan
  21. Yeom, T. H. and S. S. Park (1975), Lecture of modem Hanbang, p101, Haenglimseowon
  22. Yuk, C. S. and H. S. Yang (1999), Hyundai Saengyakhak, p133, Hakchangsa
  23. Ahrne, S., S. Nobaek, B. Jeppsson, and I. Adlerberth (1998), The normal Lactobacillus flora of healthy human rectal and oral mucosa. J. Appl. Microbiol. 85, 88-94 https://doi.org/10.1046/j.1365-2672.1998.00480.x
  24. Gusils, C., A. P. Chaia, S. Gonzalez, and G. Oliver (1999), Lactobacilli isolated from chicken intestines: potential use as probiotics. J. Food Prot. 62, 252-256 https://doi.org/10.4315/0362-028X-62.3.252
  25. Naidu, A. S., W. R. Bidlack, and R. A. Clemens (1999), Probiotic spectra of lactic acid bacteria (LAB). Crit. Rev. Food Sci. Nutr. 39, 13-126 https://doi.org/10.1080/10408699991279187
  26. Nemcova, R. (1997), Criteria for selection of Lactobacilli for probiotic use. Vet. Med. (Praha). 42, 19-27
  27. A.O.A.C. (1990), Official methods od analysis. 15th ed, p703, Association of official analytical chemists, Washington, D. C
  28. Kim, J. D., M. Y. Kim, H. J. Seo, B. J. Kim, D. H. Kim,E. O. Kim, H. Y. Chung, and J. Y. Kong (2002), Combination of Natural Products Removing ROS for Growth Promoting Effects of the Useful Enterobacteria Lacobacillus sp., Kor. J. Appl. Microbiol. Biotechnol. 30. (in press)
  29. Yuan, C. and L. J. Johnston (2000), Distribution of ganglioside GM1 in L-alpha-dipalmitoyl- phosphatidylcholine/ cholesterol monolayers: a model for lipid rafts. Biophys. J. 79, 2768-2781 https://doi.org/10.1016/S0006-3495(00)76516-7
  30. Yuan, C. and L. J. Johnston (2001), Atomic force microscopy studies of ganglioside GM1 domains in phosphatidylcholine andphosphatidylcholine/cholesterol bilayers. Biophys. J. 81, 1059-1069. https://doi.org/10.1016/S0006-3495(01)75763-3
  31. Yeo, S. G., Y. B. Park, I. S. Kim, S. B. Kim, and Y. H. Park (1995), Inhibition of xanthine oxidase by tea extracts from green tea, oolong tea and black tea. J. Kor. Soc. Food Nutr. 24, 154-159