Determination of Tyrosinase mRNA in Melanoma by Reverse Transcription-PCR and Optical Mirror Resonance Biosensor

  • Taeboo Choe (Section of Chemical and Biological Engineering, Konkuk University) ;
  • Park, Inchul (Laboratory of Cell Biology, Korea Cancer Center Hospital) ;
  • Seokil Hong (Laboratory of Cell Biology, Korea Cancer Center Hospital)
  • Published : 2002.07.01

Abstract

Tyrosinase transcript In the blood Is known as the marker of malignant melanoma and it has been often determined by using reverse transcription-polymerase chain reaction (RT-PCA) . However, after the PCR process, the quantification of amplified CDMA by the gel electrophoresis is not reliable and time-consuming. for this reason, we tried to quantify the PCR product using a cuvette-type biosensor, where the oligonucleotide probe was immobilized on the cuvette surface and the single strand CDMA, the denatured PCH product, was then hybridized onto the immobilized probe to give a response signal. The response was Immediate and takes 15 min to obtain a stable signal. The biosensor was much more sensitive comparing to the gel electrophoresis method. The quantification of PCR product using a cuvette-type biosensor was feasible and rapid.

Keywords

References

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