Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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A Study of the Growth Condition and Solubilization of Phosphate from Hydroxyapatite by Pantoea agglomeraus

  • Il Jung (Institute of Bioinidndustrial Technology, Faculty of chemical Engineering, Research Institute for Catalysis, Chonnam National University) ;
  • Park, Don-Hee (Institute of Bioinidndustrial Technology, Faculty of chemical Engineering, Research Institute for Catalysis, Chonnam National University) ;
  • Park, Kyungmoon (Institute of Bioindustrial Technology, EugeneScience)
  • Published : 2002.07.01
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Abstract

The growth conditions of Pantoea aggicmerans, a phosphate solubilizing organism, were studied In our laboratory to determine the optimal conditions. Pantoea aggionerans showed the highest growth rate at 30$\^{C}$, pH 7.0 and 2 vvm, after 50 h cultivation. A certain relationship between pH and phosphate concentration was evident when the glucose concentration in the me dium was changed. Increasing glucose concentration increased the pH buffer action of the broth. At glucose concentrations higher than the optimum concentration of 0.2 M, the cell growth was retarded. P. agglomerans consumed glucose as a substrate to produce organic acids which caused the pH decrease in the culture medium. The phosphate concentration in the medium was increased by the presence of the organic acids, which solubilized insoluble phosphates such as hydroxyapa-tite.

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References

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