Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Direct Evidence for the Radioprotective Effect of Various Carbohydrates on Plasmid DNA and Escherichia coli Cells

  • Ryu, Hwa-Ja (Department of Fine Chemical Engineering, Chonnam National University) ;
  • Yi, Kyung-Eun (Department of Faculty of Applied Chemical Engineering, Chonnam National University) ;
  • Kim, Do-Won (Department of Physics, and East Coastal Marine Bioresources Research Center, Kangnung National University) ;
  • Jung, Yun-Dae (Department of Physics, Kangnung National University) ;
  • Chang, Suk-Sang (Pohang Accelerator Laboratory, Pohang University of Science and Technology) ;
  • Seo, Eun-Seong (Department of Materials and Biochemical Engineering, Chonnam National University) ;
  • Lee, Ki-Young (Department of Faculty of Applied Chemical Engineering, Chonnam National University) ;
  • Marceau-Day, M.L. (Center for Advanced Microstructures and devices, Louisiana State University) ;
  • Kim, Do-Man (Department of Faculty of Applied Chemical Engineering, Chonnam National University)
  • Published : 2002.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Damage to cells exposed to radiation is primarily attributed to direct effects on the structure of cellular DNA. Radiation-induced damage of pBluescript SK plasmid DNA and Escherichia coli $DH5\alpha$ were examined in the presence of various branched oligosaccharides, polysaccharides, and/or 8-MOP (8-methoxypsoralen). Branched oligosaccharides efficiently protected DNA and cells exposed to ultrasoft X-ray and UV irradiation. In the presence of 0.2% (w/v) branched oligosaccharides and polysaccharides, DNA can be protected from damage due to W and ultrasoft X-ray by a factor of 1.3-2.1 fo1d and 3.2-8.3 fold, respectively. The protective effect of cells exposed to UV or ultrasoft X-ray was also observed by branched oligosaccharides. The combination of MOP, a photoreagent, with carbohydrates increased the protective effects for DNA and cells, compared with that of a single use of MOP or carbohydrate alone.

Keywords

References

  1. Br. J. Dermatol. v.144 Genital squamous cell carcinoma in men treated by photochemotherapy. A cancer registry-based study from 1978 to 1998 Aubin, F.;E. Puzenat;P. Arveux;E. Quencez;P. Humbert https://doi.org/10.1046/j.1365-2133.2001.04231.x
  2. Cellular Radiation Biology The study of radiosensitive structures with low voltage electron beams Cole, A.
  3. Curr. Med. Chem. v.8 Photochemotherapy in the treatment of cancer Dalla, V.-L.;M. S. Marciani https://doi.org/10.2174/0929867013372076
  4. Br. J. Dermatol. v.144 Cream psoralen plus ultraviolet A therapy for granuloma annulare Grundmann-Kollmann, M.;R. Ochsendirf;T. M. Zollner;I. Tegeder;R. Kaufmann;M. Podda https://doi.org/10.1046/j.1365-2133.2001.04188.x
  5. Kor. J. Appl. Microbiol. Biotechnol. v.27 Development of a mixed-culture fermentation process and characterization for new oligosaccharides and dextran using Lipomyces starkeyi and Leuconostoc mesenteroides Heo, S.-J.;D. Kim;I. S. Lee;P. S. Chang
  6. Br. J. Dermatol. v.101 5-Methoxypsoralen(bergapten) in photochemotherapy of psoriasis Honigsmann, M.;E. Jaschke;F. Gscnait;W. Brenner;P. O. Fritsch;K. Wolff https://doi.org/10.1111/j.1365-2133.1979.tb00014.x
  7. J. Microbiol. Biotechnol. v.9 Facile purification and characterization of dextransucrase from Leuconostoc mesenteroides B-512FMCM Kim, D.;D. W. Kim
  8. J. Microbiol. Biotechnol. v.10 Cloning and sequencing of the α 1→6 dextransucrase gene from Leuconostoc mesenteroides B-742CB Kim, H.;D. Kim;H. J. Ryu;J. F. Robyt
  9. J. Microbiol. Biotechnol. v.11 Production of maltopentaose and biochemical characterization of maltopentaose-forming amylase Kim, Y. M.;H. J. Ryu;S. O. Lee;E. S. Seo;S. Y. Lee;S. K. Yoo;D. L. Cho;D. Kim;A. Kimura;S. Chiba;J. Lee
  10. Int. J. Radiat. Biol. v.66 Action spectra for single- and double-strand break induction in plasmid DNA: Studies using synchrotron radiation Michael, B. D.;K. M. Prise;M. Folkard;B. Vojnovic;B. Brocklehurst;I. H. Munro;A. Hopkark https://doi.org/10.1080/09553009414551641
  11. Photochem. Photobiol. v.54 Inactivation action spectra of Bacillus subtilis spores in extended ultraviolet wavelength (50-300nm) obtained with synchrotron radiation Munakara, N.;M. Saito;K. Hieda https://doi.org/10.1111/j.1751-1097.1991.tb02087.x
  12. Proc. Natl. Acad. Sci. USA v.93 Signature p53 mutation at DNA cross-linking sites in 8-methoxypsoralen and ultraviolet A (PUVA)-induced murine skin cancers Natarag, A. J.;H. S. Black;H. N. Ananthaswamy
  13. Proc. Natl. Acad. Sci. USA v.78 Dimethyl sulfoxide prevents DNA nicking mediated by ionizing radiation or iron/hydrogen peroxide-generated hydroxyl radical Repine, J. E.;O. W. Pfenninger;D. W. Talmage;E. M. Berger;D. E. Pettijohn https://doi.org/10.1073/pnas.78.2.1001
  14. Radiat. Res. v.64 Estimation of life times and diffusion distances of radicals involved in X-ray-induced DNA strand breaks of killing of mammalian cells Roots, R.;S. Okada https://doi.org/10.2307/3574267
  15. Int. J. Radiat. Biol. v.21 Protection of DNA molecules of cultured mammalian cells from radiation-induced single-strand scissions by various alcohols and SH compound Roots, R.;S. Okada https://doi.org/10.1080/09553007214550401
  16. Arch. Dermatol. v.134 Risk squamous cell carcinoma and methoxysalen (psoralen) and UV-A radiation (PUVA): A meta-analysis Stern, R. S.;E. J. Lunder https://doi.org/10.1001/archderm.134.12.1582
  17. J. Am. Acad. Dermatol. v.44 The risk of melanoma in association with long-term exposure to PUVA Stern, R. S. https://doi.org/10.1067/mjd.2001.114576
  18. Cellular Radiation Biology Szybalski, W.;Z. Opera-Kubinska
  19. Biosci. Biotech. Biochem. v.60 Protecting effect of a green tea percolate and its main constituents against gamma ray-induced scission of DNA Yoshioka, H.;G. Akai;K. Yosinaga;K. Hasegawa;H. Yoshioka https://doi.org/10.1271/bbb.60.117
  20. Biosci. Biotech. Biochem. v.61 Protection by treahalose of DNA from radiation damage Yoshinaga, K.;H. Yoshioka;H. Kurosaki;M. Hirasawa;M. Uritani;K. Hasegawa https://doi.org/10.1271/bbb.61.160