Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Production of Recombinant Rotavirus Capsid Protein VP7 from Stably Transformed Drosophila melanogaster S2 Cells

  • Park, Jong-Hwa (Department of Genetic Engineering, Kyung Hee University) ;
  • Chang, Kyung-Hwa (Department of Genetic Engineering, Kyung Hee University) ;
  • Lee, Youn-Hyung (Department of Genetic Engineering, Kyung Hee University) ;
  • Kim, Hae-Yeong (Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University) ;
  • Yang, Jai-Myung (Department of Life Science, Sogang University) ;
  • Chung, In-Sik (Department of Genetic Engineering, Kyung Hee University, Plant Metabolism Research Center and Graduate School of Biotechnology, Kyung Hee University)
  • Published : 2002.08.01
Download PDF
⟨ Previous Next ⟩

Abstract

Stably transformed Drosophila melanogaster 52 cells producing recombinant VP7 were obtained, and recombinant VP7 expression was confirmed by Western blot analysis. The molecular weight of recombinant VP7 expressed in 52 cells was approximately 35.5 kDa, and 75% of the total VP7 produced was present in the medium. Recombinant VP7 contained N-linked glycosylated oligosaccharides. Aprotinin, leupeptin, and polyvinylpyrrolidone did not have any noticeable effect on recombinant VP7 production; however, DMSO and sodium butyrate increased its production by 120% and 60%, respectively.

Keywords

References

  1. J. Virol. v.61 Vaccinia virus recombinants expressing the SA11 rotavirus VP7 glycoprotein gene induce serotype-specific neutralizing antibodies Andrew, M. E.;D. B. Boyle;B. E. H. Coupar;P. L. Whitfed;G. W. Both;A. R. Bellamy
  2. Gene v.47 Synthesis of the outer capsid glycoprotein of the simian rotavirus SA11 in Escherichia coli Arias, C. F.;T. Ballado;M. Plebanski https://doi.org/10.1016/0378-1119(86)90065-X
  3. Biochem. J. v.310 Stimulation of tissue plasminogen activator gene expression by sodium butyrate and trichostatin A in human endothelial cells involves histone acetylation Arts, J.;M. Lansink;J. Grimbergen;K. H. Toet;T. Kooistra https://doi.org/10.1042/bj3100171
  4. Meth. Enzymol. v.91 High-sensitivity sequence analysis of proteins recovered from sodium dodecyl sulfate gels Bhown, A. S.;J. C. Bennett https://doi.org/10.1016/S0076-6879(83)91042-X
  5. J. Virol. v.65 Immunization with baculovirus-expressed recombinant rotavirus proteins VPI, VP4, VP6 and VP7 induces CD8+ T lymphocytes that mediate clearance of chronic rotavirus infection in SCID mice Dharakul, T.;M. Labble;J. Cohen;A. R. Bellamy;J. E. Street;E. R. Mackow;L. Flore;L. Rott;H. B. Greeberg
  6. Biotechnol. Prog. v.16 Process options in hepatitis B surface antigen extraction from transgenic potato Dogan, B;H. S. Mason;L. Richter;J. B. Hunter;M. L. Shuler https://doi.org/10.1021/bp0000454
  7. J. Virol. v.69 Expression of the rotavirus SA11 protein VP7 in the simple eukaryotic Dictyostelium discoideum Emslie, K. R.;J. M. Miller;M. B. Slade;P. R. Dormitzer;H. B. Greenberg;K. L. Williams
  8. J. Virol. v.61 Synthesis and immunogenicity of the rotavirus major capsid antigen using a baculovirus expression system Estes, M. K.;S. E. Crawford;M. E. Penaranda;B. L. Petrie;J. W. Burns;W. K. Chan;B. Ericson;G. E. Smith;M. D. Summers
  9. Virology(2nd ed.) Rotavirus and their replication Estes, M. K.
  10. J. Gen. Virol. v.76 Antigenicity, immunogenicity and passive protection induced by immunization of mice with baculovirus-expressed VP7 protein from rhesus rotavirus Fiore, L.;S. J. Dunn;B. Ridolfi;F. M. Ruggeri;E. R. Mackow https://doi.org/10.1099/0022-1317-76-8-1981
  11. Ann. N. Y. Acad. Sci. v.243 Effect of dimethyl sulfoxide on subunit proteins Henderson, T. R.;R. F. Henderson;J. L. York https://doi.org/10.1111/j.1749-6632.1975.tb25342.x
  12. Biotechnol. Prog. v.15 Enhancing yield of infectious bursal disease virus structural proteins in baculovirus expression systems: Focus on media, protease inhibitors and dissolved oxygen Hu, Y. C.;W. E. Bentley https://doi.org/10.1021/bp990094k
  13. J. Microbiol. Biotechnol. v.8 cDNA cloning and expression of human rotavirus outer capsid protein VP7 in insect cells Kang, D. K.;K. W. Kim;P. H. Kim;S. Y. Seoung;Y. H. Kim;I. W. Kwon;S. Y. Jeong;E. Y. Choi;K. M. Lee;H. S. Kim;E. C. Kim;A. I. Joo;J. M. Yang
  14. Virology(2nd ed.) Rotaviruses Kapikian, A. Z.;R. M. Chanock
  15. Biotechnol. Lett. v.19 no.1 The effect of polyvinylpyrrolidone (PVP) on the heavy chain monoclonal antibody from plant suspension cultures LaCount, W.;G. H. An;J. M. Lee https://doi.org/10.1023/A:1018383524389
  16. Nature v.227 Cleavage of structural proteins during the assembly of the head of bacteriophage T₄ Laemmli, U. K. https://doi.org/10.1038/227680a0
  17. Gene v.55 Expression of a major bovine rotavirus neutralization antigen (VP7) in Escherichia coli McCrane, M. A.;J. G. McCorquodale https://doi.org/10.1016/0378-1119(87)90243-5
  18. Virus Res. v.23 Expression of the gene coding for the major outer capsid protein of SA11 rotavirus in a baculovirus system McGonigal, T. D.;M. C. Turon;K. Komar-Hartnett;S. E. Kister;R. E. Smith https://doi.org/10.1016/0168-1702(92)90073-I
  19. Adv. Virus Res. v.44 Rotaviruses; Immunological determinant of protection against infection and disease Offit, P. A. https://doi.org/10.1016/S0065-3527(08)60329-2
  20. Enzyme Microb. Technol. v.25 Optimization of transfection conditions for expression of green fluorescent protein in Drosophila melanogaster S2 cells Park, J. H.;H. Y. Kim;K. H. Han;I. S. Chung https://doi.org/10.1016/S0141-0229(99)00096-4
  21. Vet. Microbiol. v.37 Immunogens of rotaviruses Paul, P. S.;Y. S. Lyoo https://doi.org/10.1016/0378-1135(93)90031-2
  22. J. Lipid Research v.36 Secretion of biologically active human proapoliprotein A-1 on a baculovirus-insect cell system: Protection from degradation by protease inhibitors Pyle, L. E.;P. Banton;Y. Fujiwara;A. Mitchell;A. Fidge
  23. Biotech. Bioeng. v.33 Media and environmental effects on phenylics production from Tobacco cell cultures Schmidt, A. J.;J. M. Lee;G. An https://doi.org/10.1002/bit.260331111
  24. Biotechnol. Lett. v.17 no.5 Effects of dimethyl sulfoxide on heavy chain monoclonal antibody production from plant cell culture Wahl, M. F.;G. H. An;J. M. Lee https://doi.org/10.1007/BF00132011
  25. Mol. Cell. Biol. v.7 Location of signal sequence within the rotavirus SA11 glycoprotein VP7 which direct it to the endoplasmic reticulum Whitfeld, P. L.;C. Tyndall;S. C. Stizaka;A. R. Bellamy;G. W. Both https://doi.org/10.1128/MCB.7.7.2491