Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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On-line Conversion Estimation for Solvent-free Enzymatic Esterification System with Water Activity Control

  • Lee, Sun-Bok (Department of Chemical Engineering, Division of Molecular and Life Science, and Institute of Environmental and Energy Technology, Pohang University) ;
  • Keehoon Won (Department of Chemical Engineering, Division of Molecular and Life Science, and Institute of Environmental and Energy Technology, Pohang University)
  • Published : 2002.03.01
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Abstract

On-line conversion estimation of enzymatic esterification reactions in solvent-free media was investigated. In principle, conversion to ester can be determined from the amount of water produced by the reaction, because water is formed as a by-product in a stoichiometric manner. In this study, we estimated the water production rate only from some measurements of relative humidity and water balances without using any analytical methods. In order to test the performance of the on-line conversion estimation, the lipase-catalyzed esterification of n-capric acid and n-decal alcohol in solvent-free media was performed whilst controlling water activity at various values. The reaction conversions estimated on-line were similar to those determined by offline gas chromatographic analysis. However, when the water activity was controlled at higher values, discrepancies between the estimated conversion values and the measured values became significant. The deviation was found to be due to the inaccurate measurement of the water content in the reaction medium during the initial stages of the reaction. Using a digital filter, we were able to improve the accuracy of the on-line conversion estimation method considerably. Despite the simplicity of this method, the on-line estimated conversions were in good agreement with the off-line measured values.

Keywords

References

  1. Nature v.409 Improving enzymes by using them in organic solvents Klibanov, A.M. https://doi.org/10.1038/35051719
  2. Curr. Op. Chem. Biol. v.5 Industrial biocatalysis Zaks, A. https://doi.org/10.1016/S1367-5931(00)00181-2
  3. Anal. Lett. v.22 Biothermal analysis performed in organic solvents Danielsson, B.;L. Flygare https://doi.org/10.1080/00032718908051609
  4. Biotechnol. Tech. v.3 The use of biosensors in organic synthesis: peptide synthesis by immobilized α-chymotrypsin assessed with an enzyme thermistor Stasinska, B.;B. Danielsson;K. Mosbach https://doi.org/10.1007/BF01876065
  5. Enzyme Microb. Technol. v.20 On-line monitoring of enzyme-catalyzed biotransformations with biosensors Lammers, F.;T. Scheper https://doi.org/10.1016/S0141-0229(96)00171-8
  6. Biotechnol. Bioeng. v.51 NMR on-line monitoring of esterification catalyzed by cutinase Sarazin, C.;F. Ergan;J.-P. Seguin;G. Goethals;M.-D. Legoy;J.-N. Barbotin https://doi.org/10.1002/(SICI)1097-0290(19960920)51:6<636::AID-BIT3>3.0.CO;2-I
  7. Tiends Biotechnol. v.18 Biotransformations monitored in situ by proton nuclear magnetic resonance spectroscopy Brecker, L.;D.W. Ribbons https://doi.org/10.1016/S0167-7799(00)01425-6
  8. Curr. Op. Biotechnol. v.11 Online NMR for monitoring biocatalysed reactions Weber, H.;L. Brecker https://doi.org/10.1016/S0958-1669(00)00146-4
  9. Biotechnol. Bioeng. v.19 Computer-aided baker's yeast fermentations Wang, H.Y.;C.L. Cooney;D.I.C. Wang https://doi.org/10.1002/bit.260190107
  10. AIChE J. v.24 Real-time estimation of aerobic batch fermentation biomass concentration by component balancing Zabriskie, D.W.;A.E. Humphrey https://doi.org/10.1002/aic.690240116
  11. Ann. NY Acad. Sci. v.369 Material balancing applied to the prediction of glutamic acid production and cell mass formation Constantinides, A.;P. Shao https://doi.org/10.1111/j.1749-6632.1981.tb14186.x
  12. Biotechnol. Bioeng. v.33 Fermentation data-analysis and state estimation in the presence of incomplete mass balance Liao, J.C. https://doi.org/10.1002/bit.260330515
  13. Cytotechnology v.29 Estimation of rates of oxygen uptake and carbon dioxide evolution of animal cell culture using material and energy balances Xiu, Z.-L.;W.-D. Deckwer;A.-P. Zeng https://doi.org/10.1023/A:1008004618163
  14. Biotechnol. Bioeng. v.57 Effect of salt hydrate pair on lipase-catalyzed regioselective monoacylation of sucrose Kim, J.E.;J.J. Han;J.H. Yoon;J.S. Rhee https://doi.org/10.1002/(SICI)1097-0290(19980105)57:1<121::AID-BIT15>3.0.CO;2-N
  15. Biotechnol. Bioeng. v.63 Influence of water activity on the enantioselective esterification of (R,S)-ibuprofen by Candida antarctica lipase B in solventless media Pepin, P.;R. Lortie https://doi.org/10.1002/(SICI)1097-0290(19990520)63:4<502::AID-BIT14>3.0.CO;2-O
  16. J. Am. Oil Chem. Soc. v.76 Continuous lipase-catalyzed production of wax ester using silicone tubing Wehtje, E.;D. Costes;P. Adlercreutz https://doi.org/10.1007/s11746-999-0190-4
  17. Biotechnol. Prog. v.17 Computer-aided control of water activity for lipase-catalyzed esterification in solvent-free systems Won, K.;S.B. Lee https://doi.org/10.1021/bp000154i
  18. Chem. Eng. Sci. v.52 Prediction of water adsorption curves for heterogeneous biocatalysis in organic and supercritical solvents Condoret, J.-S.;S. Vankan;X. Joulia;A. Marty https://doi.org/10.1016/S0009-2509(96)00413-7
  19. Lipids v.34 Enhancement of both reaction yield and rate of synthesis of structured triacylglycerol containing eicosapentaenoic acid under vacuum with water activity control Han, J.J.;T. Yamane https://doi.org/10.1007/s11745-999-0449-6
  20. Biopolymers v.33 The hydration of protein in nearly anhydrous organic solvent suspensions McMinn, J.H.;M.J. Sowa;S.B. Charnick;M.E. Paulaitis https://doi.org/10.1002/bip.360330808
  21. Biotechnol. Bioeng. v.49 The role of hydration in enzyme activity and stability: 1. water adsorption by alcohol dehydrogenase in a continuous gas phase reactor Yang, F.;A.J. Russell https://doi.org/10.1002/(SICI)1097-0290(19960320)49:6<700::AID-BIT12>3.0.CO;2-9
  22. J. Am. Chem. Soc. v.60 Adsorption of gases in multimolecular layers Brunauer, S.;P.H. Emmett;E. Teller https://doi.org/10.1021/ja01269a023
  23. J. Ferment. Bioeng. v.79 Effect of water activity on enzyme hydration and enzyme reaction rate in organic solvents Lee, S.B.;K.-J. Kim https://doi.org/10.1016/0922-338X(95)91264-6
  24. Biotechnol. Tech. v.10 A new method to determine the aw range in which immobilized lipases display optimum activity in organic media Arroyo, M.;J.M. Sanchez-Montero;J.V. Sinisterra
  25. Biotechnol. Lett. v.18 Water adsorption isotherm as a tool to predict the preequilibrium water amount in preparative esterification De la Casa, R.M.;J.M. Sanchez-Montero;J.V. Sinisterra https://doi.org/10.1007/BF00137803
  26. Pons MN. Bioprocess monitoring and control Process control and optimization Lim, H.C.;K.S. Lee
  27. Process Dynamics and Control Seborg, D.E.;T.F. Edgar;D.A. Mellichamp
  28. ISA Trans. v.38 Digital filtering in the control of a batch distillation column Oisiovici, R.M.;S.L. Cruz;J.A.F.R. Pereira https://doi.org/10.1016/S0019-0578(99)00024-5