Journal of Environmental Health Sciences (한국환경보건학회지)

Korean Society of Environmental Health (한국환경보건학회)
권호 표지

Uptake and Elimination of 2,3,7,8-tetrachlorodibenzo-p-dioxin(TCDD) and 3,3 ,4,4 ,5-pentachlorobiphenyl(PCB126) in Japanese Medaka(Oryzias latipes)

일본송사리에서 TCDD 및 PCB 126의 흡수 및 배설

  • 김영철 (계명대학교 자연과학부 공중보건학과)
  • Published : 2002.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

초기생애단계의 일본송사리를 실험어류로 사용하여 TCDD와 PCB 126의 생물농축정도를 비교 측정하였고, TCDD와 PCB 126의 체외 배설에 대한 동태학적 특성을 비교 분석하였다. 생물농축실험은 초기생애단계의 새끼치어 및 어린 물고기에 TCDD와 PCB 126을 여러 농도로 물에 용해시켜 96시간 동안 정체된 상태로 폭로시킨 후 생물농축계수(BCF)와 지방-표준화한 생물농축계수(BCF$_{L}$)를 측정하였다. TCDD와 PCB 126의 일본송사리 초기생애단계의 새끼치어 및 어린 물고기에서 측정된 96-h BCF값 및 96-h BCF$_{L}$값은 폭로된 농도와 상관없이 비슷하게 나타나 반면, 생체크기가 클수록 그 값은 작게 나타났다. 일본송사리 새끼치어, 어린 물고기, 성어의 생체 내 총 지방함량은 각각 5.7, 4.2, 4.6%로 측정되었다. 체외배설 동태실험은 어린 물고기에 TCDD와 PCB 126을 3가지 농도롤 물에 용해시켜 96시간 동안 정체된 상태로 폭로시킨 후, flow-through장치로 공급되는 맑은 물로 옮겨 42일간 사양하면서 0, 7, 14, 21, 28, 42일에 생체 내 TCDD와 PCB 126 잔류량을 측정하여 분석하였다. 배설은 1차 반응속도론적으로 이루어졌으며 한 구획모형보다 두 구획모형이 더 적합한 것으로 분석되었다. 두 구획모형을 사용한 분석에서 TCDD와 PCB 126의 체외배설 반감기(t$\frac{1}{2}$) 및 배설속도상수($\beta$)는 각각 27.2일과 32.3일 및 0.025/d와 0.021/d로 측정되었다.

Keywords

References

  1. Smith, L.M., Schwartz, T.R., Feltz, K., and Kubiak, T.J. : Determination and occurrence of AHH-active polychlorinated biphenyl, 2 ,3 ,7, 8-tetrachlorodibenzo-p-dioxin and 2, 3, 7, 8-tetrachlorodibenzofuran in Lake Michigan sediment and biota: The question of their relative toxicological significance. Chemosphere, 21, 1063-1085, 1990 https://doi.org/10.1016/0045-6535(90)90128-G
  2. Kim, Y. and Cooper, K.R. : Interactions of 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin (TCDD) and 3, 3', 4, 4', 5-pentachlorobiphenyl(PCB 126) for producing lethal and sublethal effects in the Japanese medaka embryos and larvae. Chemosphere, 36(2), 409-418, 1998 https://doi.org/10.1016/S0045-6535(97)10066-2
  3. Kim, Y. and Cooper, K.R. : Toxicity of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) and polychlorobiphenyls(PCBs) in the embryos and newly hatched larvae of the Japanese medaka(Oryzias latipes). Chemosphere. 39(3), 527-538, 1999 https://doi.org/10.1016/S0045-6535(98)00603-1
  4. Kim, Y. : Toxic potency of 3, 3', 4, 4', 5-pentachlorobiphenyl(PCB 126)relative to 2, 3, 7, 8-tetrachlorodibenzo-p-dioxin(TCDD) in the Japanese medaka early life stage assay, J Toxicol. Pub. Health, 15(2). 141-147, 1999
  5. Kim, Y. : Interactions of TCDD and PCB 77, and PCB 77 and PCB 126 In Japanese medaka(Oryzias latipes) embryos. J. Toxicol. Pub. Health, 15(2), 149-154, 1999
  6. Lien, G.J. and Mckim, J.M. : Predicting branchial and cutaneous uptake of 2, 2', 5, 5' -tetrachlorobiphenyl in fathead minnows(Pimephales promelas) : Rate limiting factors. Aquat. Toxicol., 27, 15-32, 1993 https://doi.org/10.1016/0166-445X(93)90044-2
  7. Kleeman, J.M., Olson, J.R., Chen, S.M., and Peterson, R.E.: Metabolism and disposition of 2, 3 ,7, 8,-tetrachlorodibenzo-p-dioxin in rainbow trout. Toxicol. Appl. Pharmacol., 83. 391-401, 1986 https://doi.org/10.1016/0041-008X(86)90221-8
  8. Kleeman, J.M., Olson, J.R., Chen, S.M., and Peterson, R.E. : 2, 3, 7, 8,-tetrachlorodibenzo-p-dioxin metabolism and disposition in yellow perch. Toxicol. Appl Pharmacol, 83, 402-411, 1986 https://doi.org/10.1016/0041-008X(86)90222-X
  9. U.S. Environmental Protection Agency, Standard operating procedure for tissue-specific determination of total lipid content, Appendix 10. 20. Mid-Continent Ecology Division, Duluth, Minn. 1993
  10. Gibaldi, M and Perrier, D. : Multicompartment models, in Pharmacokinetics. Marcel Dekker, Inc., New York, 45-96, 1975
  11. Mackay, D. : Correlations of bioconcentration factors. Environ. sci. Technol., 16, 274-278, 1982 https://doi.org/10.1021/es00099a008
  12. Hawker, D.W. and Connell, D.W. : Octanol-water partition coefficients of polychlorinated biphenyl congeners. Environ. Sci. Technol, 22, 382-387, 1988 https://doi.org/10.1021/es00169a004
  13. Adams, W.J., DeGraeve, G.M., Sabourin, T.D., Cooney, J.D., and Mosher, G.M.: Toxicity and bioconcentration of 2, 3, 7, 8,-TCDD to fathead minnows(Pimephales promelas). Chemosphere, 15, 1503-1511, 1986 https://doi.org/10.1016/0045-6535(86)90431-5
  14. Opperhuizen, A. and Sijm, D.T.H.M. : Bioaccumulation and biotransformation of polychlorinated dibenzo-p-dioxin and dibenzofurans in fish. Environ. Toxicol. Chem., 9, 175-186, 1990 https://doi.org/10.1897/1552-8618(1990)9[175:BABOPD]2.0.CO;2
  15. Huges, G.M. : General anatomy of the gills, in fish physiology. Vol. 10A, Hoar, W.S. and Randall, D.J., Eds., Academic press, Orlando, FL., 1-72, 1984
  16. Hayton, W.L. : Rate-limiting barriers to intestinal drug absorption: A review. J. Pharmacok. Biopharm., 8, 321-334, 1980 https://doi.org/10.1007/BF01059381
  17. Rombough, P.J. and Moroz, B.M. : The scaling and potential importance of cutaneous and branchial surfaces In respiratory gas exchange in young chinook salmon (Oncorhynchus tshawytscha). J. Exp. Biol., 154, 1-12, 1990
  18. Saarikoski, J., Lindstrom, R., Tyynela, M., and Viluksela, M. : Factors affecting the absorption of phenolics and carboxylic acids in the guppy (Poecilia reticulata). Ecotoxicol. Environ. Safety, 11, 158-173, 1986 https://doi.org/10.1016/0147-6513(86)90060-6
  19. Mckim, J.M. and Nichols, J.W. : Physiologically based toxicokinetic modeling of the dermal uptake of three waterborne chloroethanes in rainbow trout (Oncorhynchus mykiss). Abstr. Toxicologist, 11, 35, 1991
  20. Schmieder, P., Lothenbach, D., Tietge, J., Erickson, R., and Johnson, R. : $[^3H]$-2, 3, 7, 8-TCDD uptake and elimination kinetics of medaka (Oryzias latipes). Environ. Toxicol. Chem., 14, 1735-1743, 1995 https://doi.org/10.1897/1552-8618(1995)14[1735:HUAEKO]2.0.CO;2
  21. Barron, M.G, Stehly, G.R, and Hayton, W.L. : Pharmacokinetic modeling in aquatic animals I. Models and concepts. Aquat. Toxicol., 18, 61-86, 1990 https://doi.org/10.1016/0166-445X(90)90019-L
  22. Mehrle, P.M., Buckler, D.R., Little, E.E., Smith, L.M., Petty, J.D., Peterman, P.H., Stalling, D.L., DeGraeve, G.M., Coyle, J.J., and Adams, W.J. : Toxicity and bioconcentration of 2, 3, 7, 8,-tetrachlorodibenzo-p-dioxin and 2, 3, 7, 8-tetrachloro-dibenzofuran in rainbow trout. Environ. Toxicol. Chem., 7, 47-62, 1988 https://doi.org/10.1897/1552-8618(1988)7[47:TABOTA]2.0.CO;2
  23. Leblanc, G.A. : Trophic-level differences in the bioconcentration of chemicals: Implication In assessing environmental biomagnification. Environ. Sci. Technol., 29, 154-160, 1995 https://doi.org/10.1021/es00001a020