Glycolipid Biosurfactants Produced by Pseudomonas aeruginosa D2D2 from Diesel-Contaminated Soil

  • MOON, HYE-JOON (Research Institute for Clean Technology, Myong-Ji University) ;
  • YOUNG-KUONG LIM (Department of Environmental Engineering and Biotechnology, Myong-Ji University) ;
  • HEE-SIK KIM (Environmental Microbiology R.U., Korea Research Institute for Bioscience and Biotechnology) ;
  • DAE-YOUNG KWON (Department of Environmental Engineering and Biotechnology, Myong-Ji University) ;
  • WOOK-JIN CHUNG (Department of Environmental Engineering and Biotechnology, Myong-Ji University)
  • Published : 2002.06.01

Abstract

A biosurfactant-producing bacterial strain was selected from diesel-contaminated soil by measuring the oil-film collapsing activity and identified as Pseudomonas aeruginosa D2D2. When glucose and olive oil were used as carbon sources, 11.46 g/1 of biosurfactant was obtained. Based on TLC analysis, the biosurfactant produced from P. aeruginosa D2D2 was identified as a glycolipid, consisting of two types of biosurfactants (Type I and Type II). The purified glycolipid reduced the surface tension of the culture from 72 dyne/cm to 27 dyne/cm. The hydrophilic and hydrophobic moiety of the biosurfactant were rhamnose and ${\beta}$-hydroxydecanoic acid, as determined by FAB-MS and NMR analyses, respectively.

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