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The Korean Society of Plant Pathology (한국식물병리학회)
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Phylogenetic rind Taxonomic Status of the Phytoplasmas Associated with Water Dropwort (Oenanthe javanica DC) Disease in Korea and Japan

  • Jung, Hee-Young (Department of Agricultural and Environmental Biology, The University of Tokyo) ;
  • Woo, Tae-Ha (Research Center for New Bio-Materials in Agriculture, Seoul National University) ;
  • Hibi, Tadaaki (Department of Agricultural and Environmental Biology, The University of Tokyo) ;
  • Namba, Shigetou (Laboratory of Bioresource Technology, Graduate School of Frontier Sciences, The University of Tokyo) ;
  • Lee, Joon-Tak (Department of Agricultural Biology, College of Agriculture and Life Sciences, Kyungpook National University)
  • Published : 2002.06.01
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Abstract

To evaluate the phylogenetic and taxonomic status of the phytoplasmas associated with water dropwort (Oenanthe javanica DC) disease in Korea and Japan, their 16S rDNA was analyzed. DNAs extracted from water dropworts collected in Korea (Kyongnam province) and Japan (Chiba prefecture) affected by witches' broom and yellows were subjected to PCR using phytoplasma-specific primers, which amplified a 1.4-kbp fragment that included the 16S rDNA. Phytoplasmas were characterized by RFLP analysis using AluI, HaeIII, HhaI, KpnI, MseI, and RsaI restriction enzymes and by sequence analysis of the PCR products. The mater dropwort witches'broom (WDWB) and water dropwort yellows (WDY) 16S rDNA sequences were identical and closely related to onion yellows (OY, 99.9% identity), which belong to the aster yellows (AY) 16S-subgroup. However, the KpnI RFLP analyses clearly distinguished the WDY and WDWB phytoplasmas from the OY phytoplasma. The phylogenetic analysis based on 16S rDNA showed that WDWE and WDY phytoplasmas are members of a relatively homogeneous group that evolved from a common ancestor.

Keywords

References

  1. Jung, H.-Y, Sawayanagi, T., Kakizawa, S., Nishigawa, H., Oshima, K., Miyata. S., Ugaki, M., Lee, J.-T., Hibi, T. and Namba, S. 2002. 'Candidatus Phytoplasma castaneae', a novel phytoplasma taxon associated with chestnut witches' broom disease, Int. J. Syst. Evol. Microbiol. (in press)
  2. Kimura, M. 1980. A simple method for estimating evolutionaly rates of base substitutions through comparative studies of nucleotides sequences. J. Mol. Evol. 16:111-120 https://doi.org/10.1007/BF01731581
  3. Kirkpathck, B. C. 1992. Mycoplasma-like organisms: Plant and invertebrate pathogens, In: The prokaryotes. 2nd ed. by A. Balows., H. G. Truper., M. Dworkin., W. Harder and K. H. Schleifer, pp. 4050-4067., Sprmger-Verlag, New York
  4. Lee, I. M., Hammond, R. W., Davis, R. E. and Gundersen, D. E. 1993. Universal amplification and analysis of pathogen 16S rDNA for classification and identification ofmycoplasma like organisms. Phytopathotogy 83:834-842 https://doi.org/10.1094/Phyto-83-834
  5. Lee, I. M., Dawn, E., Gundersen, D. E., Davis, R. E. and Bar-toszyk, I. M. 1998. Revised c1assi6cation scheme ofphyto-plasmas based on RFLP analyses of 16S rRNA and ribosomal protain gene sequences, Int. J. Syst. Bacteriot. 48:1153-1169 https://doi.org/10.1099/00207713-48-4-1153
  6. Lee, J.-T. and Woo, T.-H. 1999. On the occurrence of water drop-wort witches' broom in Yeungam area. Korean J. Mycoptas-mology 10:59 (Abstr. in K-orean)
  7. Liefting, L. W., Andersen, M. T., Beever, R. E., Gardner, R. C. and Foster, L. S. 1996. Sequence heterogeneity in the two 16S rRNA genes of Phonnium yellow leaf phytoplasma. Appl. Environ. Microbiol. 62:3133-3139
  8. Namba, S., Kato, S., Iwanami, S., Oyaizu, H., Shiozawa, H. and Tsuchizaki, T. 1993a. Detection and differentiation of plant-pathogenic mycoplamalike organisms using polymerase chain reaction. Phytopathology 83:786-791 https://doi.org/10.1094/Phyto-83-786
  9. Namba, S., Oyaizu, H., Kato, S., Iwanami, S. and Tsuchizaki, T. 1993b. Phylogenetic diversity of phytopathogenic mycoplas-malike organisms, Int. J. Syst. Bacteriol. 43:461-467 https://doi.org/10.1099/00207713-43-3-461
  10. Saitou, N. and Nei, M. 1987. Neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol. Biol. Evol. 4:406-425
  11. Sawayanagi, T, Hinkoshi, N., Kanehira, T., Shinohara, M., Bert-accmi. A., Cousin, M-T., Hiruki, C. and Namba, S. 1999. 'Candidatus Phytoplasma japonicum', a new phytoplasma taxon associated with Japanase Hydrangea phyllody. Int. J. Syst. Bacteriol. 49:1275-1285 https://doi.org/10.1099/00207713-49-3-1275
  12. Schneider, B. and Seemuller, E. 1994. Presence of two sets of ribosomal genes in phytopathogenic mollicutes. Appl. Envi-ron. Microbiol. 141:173-185
  13. Shiomi, T. and Sugiura, M. 1983. Water dropwort yellows and chrysanthemum witches' broom occurred in Ishikawa Prefechire. Ann. Phytopathot. Soc. Jpn. 49:367-370 https://doi.org/10.3186/jjphytopath.49.367
  14. Thompson, J. D., Higgins, D. G. and Gibson, T. J. 1994. CLUSTAL W : improving the sensitivity of progressive multiple sequence alignment through sequence weighting, Position specific gap penalties and weight matrix choice. Nucleic Acids Res. 22:4673-4680 https://doi.org/10.1093/nar/22.22.4673
  15. Woo, T. H. 2000. Diagnosis and occurrence ecology on witches' broom disease of Oenanthe javanica DC. Kyungpook National University master thesis, p. 29 (in Korean with English abstr.)

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