International Journal of Industrial Entomology and Biomaterials

Korean Society of Sericultural Science (한국잠사학회)
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Molecular Cloning of Cytochrome P450 Family Gene Fragment from Midgut of the Beet Armyworm, Spodoptera exigua

  • Moon, Jae-Yu (School of Biological Resources and Material Engineering, College of Agriculture and science, Seoul National) ;
  • Lee, Pyeongjae (School of Biological Resources and Material Engineering, College of Agriculture and science, Seoul National) ;
  • Cho, Il-Je (School of Biological Resources and Material Engineering, College of Agriculture and science, Seoul National) ;
  • Kim, Iksoo (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆Technology, Development Rural Develop) ;
  • Lee, Heui-Sam (Department of Sericulture and Entomology, The National Institute of Agricultural Science ＆Technology, Development Rural Develop)
  • Published : 2002.06.01
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Abstract

Cytochrome P45O (CYP) gene has been known to play one of the most important roles in metabolizing the exogenous materials. In insect, CYP is particularly known to detoxify toxic materials by adding oxygen molecule to the hydrophobic region of the materials. Thus, CYP-dependent metabolism is associated with the adaptation of insect to host plant chemicals. This in turn is known to be one of the driving forces for CYP diversification. In the present study, we cloned seven gene fragments of CYP 4 (CYP4) family from the midgut of the beet armyworm, Spodoptera exigua, through RT.PCT, Sequence analysis of the product showed the gene fragment to contain an open reading frame of ~150 amino acids, consisted of ~450 bp. The cloned gene fragments contained typical, conserved regions found in CYP4 family. Pairwise comparison of the deduced amino acid sequences among seven clones ranged in divergence from 0% to 52.86% and resulted in five distinct clones. The other two clones were identical or differ by one amino acid respectively to the corresponding clone, although each differed by ten nucleotides. Analysis of correlation between GenBank-registered, full length CYP4 and the cloned fragments resulted in statistically significant relationship ($r^{2}$ = 0.96085; p < 0.001), suggesting utility of the partial sequences as such full-length sequences. Phylogenetic analysis of the clones with GenBank-registered insect and mammal CYP4 family sequences by parsimony and several distance methods subdivided the clones into two groups: tones belonging to CYP4S and the others to CYP4M families.

Keywords

References

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