Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Production of Microbial Pesticides by Soybean Curd Waste-water in Bacillus thuringiensis subsp. kurstaki HD-1

Bacillus thuringiensis kurstaki HD-1 유래 미생물살충제 생산을 위한 두부공업폐수의 이용

  • Ok, Min (Faculty of Natural Resource and Life Science, Dong-A University) ;
  • Kim, Dae-Jin (Faculty of Food and Nutrition, Dong-A University) ;
  • Lee, Young-Chun (Faculty of Natural Resource and Life Science, Dong-A University) ;
  • Choi, Yong-Lak (Faculty of Natural Resource and Life Science, Dong-A University) ;
  • Cho, Young-Su (Faculty of Natural Resource and Life Science, Dong-A University)
  • 옥민 (동아대학교 생명자원과학부) ;
  • 김대진 (동아대학교 식품과학부) ;
  • 이영춘 (동아대학교 생명자원과학부) ;
  • 최용락 (동아대학교 생명자원과학부) ;
  • 조영수 (동아대학교 생명자원과학부)
  • Published : 2002.06.01
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Abstract

The waste-water from the industry for production of a soybean curd (the soybean curd waste-water) was investigated to use for the substrate to produce the endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1 used as one of well known microbial pesticides. The pH of the soybean curd waste-water was 9.8 and its chemical oxygen demand (COD), total nitrogen (TN) and phosphate (TP) were 276.0, 71.1 and 5.5mg/$\ell$, respectively. The higher was the concentration of the soybean curd waste-water in the medium, the more endotoxin was produced. Maximal sporulation occurred at which concentration of $K_2$HPO$_4$in the medium supplied with the soybean curd waste-water was 1% (w/v). Production of the endotoxin with the optimized medium supplied with the soybean curd waste-water was 1.5 times higher than that without the soybean curd waste-water. The soybean curd waste-water was found to be suitable substrate for production of the endotoxin of Bacillus thuringiensis subsp. kurstaki HD-1.

Bacillus thrungensis를 이용한 미생물 살충제를 생산할 목적으로 두부폐수를 이용하여 내생포자 생산능을 검토하였다. 실험에 사용한 두부폐수원액의 성상은 pH 9.8, COD 276mg/$\ell$, 전질소량 71.1mg/$\ell$, 전인량 5.52mg/$\ell$ 이며 Bacillus thrungensis균을 대량배양하기 위한 기질로서의 이용 가능성을 확인한 결과, 두부폐수원액에서 가장 높은 포자형성능이 관찰 되었으며, 두부폐수원액의 첨가량이 작을수록 포자형성은 감소하는 경향을 나타내었다. 최대 포자형 성능은 1% $K_2$HPO4i 첨가때 가장 높은 포자형 성능을 나타내었다. 두부 공업폐수를 이용한 Bacillus thrungensis의 최적배지 조건에서 시간별 포자형 성능을 확인한 결과, 동일 시간당 1.5배 높은 포자형 성능이 확인되었다. 두부폐수는 Bacillus thrungensis를 생산하기 위한 적당한 배양기질로서 이용될 수 있을 것으로 생각되어 진다.

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