Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Study of Functional Chungkukjang contain Fibrinolytic Enzyme

혈전용해효소함유 기능성 청국장제조에 관한 연구

  • 류충호 (경상대학교 응용생명과학부) ;
  • 김익조 (경상대학교 응용생명과학부) ;
  • 김형갑 (진주산업대학교 환경공학과) ;
  • 정종화 (경남보건환경연구원 동의대학교 미생물학과) ;
  • 정영기 (경남보건환경연구원 동의대학교 미생물학과)
  • Published : 2002.06.01
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Abstract

A bacterial strain showing the fibrinolytic activity was screened from korean traditional soybean products. For the identification, the strain was investigated morphology and biochemical characteristics and it was classified to Bacillus subtilis. The strain had high fibrinolytic activity in Chungkukjang. The optimum fermentation condition of temperature and time were 37$^{\circ}C$ and 24hour. The pH in Chungkukjang was gradually alkalized during fermentation. The fibrinolytic enzyme in Chungkukjang stable at heft treatment; After heating at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 30 min, the fibrinolytic activity remained 75% and 40%, respectively.

전통 콩 발효식품으로부터 혈전용해능이 뛰어난 균주를 분리하였다. 이 균주의 형태학적, 생화학적 특성을 조사한 결과 Bacillus subtilis로 동정되었다. 분리된 균주를 이용하여 혈전용해활성이 높은 청국장 제조를 위한 최적발효조건은 37$^{\circ}C$에서 24시간이었다. 증자대두는 초기 pH 6.3에서 12, 24 및 48시간동안 발효시킨 각각의 pH는 6.31, 7.24, 7.81로 약 알칼리화 되었다. 본 혈전용해효소는 열안정성이 높아 6$0^{\circ}C$ 와 8$0^{\circ}C$에서 30분 처리하였을 때 혈전용해활성을 각각 75%와 와 40% 유지하였다.

Keywords

References

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