Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Characterization of Remazol Black B-Biodegradation by Stenotrophomonas maltophilia EJ-211

Stenotrophomonas maltophilia EJ-211에 의한 Remazol Black B의 분해 특성 분석

  • 이은열 (경성대학교 공과대학 식품공학과) ;
  • 이은정 (경성대학교 공과대학 식품공학과) ;
  • 임광희 (대구대학교 화학공학과) ;
  • 임동준 (영남대학교 화학공학과)
  • Published : 2002.06.01
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Abstract

A reactive dye-degrading bacterium was isolated from textile waste water and it was identified as Stenotrophomonas maltophilia based on its biochemical characteristics. The effects of additional carbon and nitrogen sources were investigated for the development of an optimal medium for biodegradation of Remazol Black B by 5. maltophilia. The optimal pH and temperature were determined to be 6.5 and 3$0^{\circ}C$, respectively. Remazol Black B with the initial concentration of 50 ppm could be degraded up to 86% within 28 h.

염색 공단 폐수로부터 채취한 시료에서 Remazol Black B를 유일 탄소원으로 성장할 수 있는 미생물들을 순수 분리 하고, 염료 분해능이 가장 우수한 균주를 API 20E kit를 이용해 생화확적 특성을 분석해 본 결과, Stenotrophomonas maltophilia EJ-211로 명명하였다. S. maltophilia EJ-211에 의한 난분해성 반응염료인 Remazol Black B 분해 반응을 분석한 결과, 보조 탄소원으로 포도당 1.0%(w/v), 질소원으로 trypton peptone은 1.0%(w/v), 3$0^{\circ}C$, pH 6.5의 최적 조건을 결정할 수 있었다. 호기적 조건에서의 S. maltophilia EJ-211의 Remazol Black B는 28시간 동안 약 86% 수준의 염료 분해능을 보여주어 Remazol Black B에 대한 생분해능이 아주 우수함을 확인할 수 있었다.

Keywords

References

  1. Biores. Technol v.58 Microbial decolorization of textile-dye-containing effluents: a review Banat, I. M., P. Nigam, D. Singh;R. Marchant https://doi.org/10.1016/S0960-8524(96)00113-7
  2. Appl. Environ. Microbiol v.64 Isolation of a bacterial strain with the ability to utilize the sulfonated azo compround 4-carboxy-4-sulfoazobenzene as sole source of carbon and energy Blumel, S., M. Contzen, M. Lutz, A. Stolz;H. J. Knackmuss
  3. Water Res v.34 Decolorization of an azo dye by unacclimated activated sludge under anaerobic conditions Bromley-Challenor, K. C. A., J. S. Knapp, Z. Zhagn, N. C. C. Gray, M. J. Hetheridge;M. R. Evans https://doi.org/10.1016/S0043-1354(00)00212-8
  4. Crit. Rev. Environ. Sci. Technol v.56 Predicting azo dyes Brown, M. A;S. C. DevVito
  5. Biotechnol. Prog v.16 Fed-batch bioreactor strategies for microbial decolorization of azo dye using a Pseudomonas Luteola strain Chang, J. S;Y. C. Lin https://doi.org/10.1021/bp000116z
  6. Environ. Technol v.15 Characterization of tezile wastewaters: a review Correia, V. M., T. Stephenson;S. J. Judd https://doi.org/10.1080/09593339409385500
  7. Water Sci. Tech v.36 Characerization of aerobic azo dye-degrading bacteria and their activity in biofilms Coughlin, M. F., B. K. Kinkle, A. tepper;P. L. Bishop
  8. J. Bioshi. Bioeng v.90 Isolation and characterization of Aeromonas sp. B-5 capable of decolorizing various dyes Hayase, N., K. Kouno;K. Ushio https://doi.org/10.1016/S1389-1723(01)80044-X
  9. FEMS Microbiol. Lett v.188 Decolurisation of synthetic texile dyes by Phleia tremellosa Kiby, N., R. Marchant;G. McMullan https://doi.org/10.1111/j.1574-6968.2000.tb09174.x
  10. Biotechnol. Lett v.18 Decolourisation and metabolism of the reactive texile dye Remazol Black B, by an immobilized microbial consortium Oxspring, D. A., G. McMullan, W. F. Smyth;R. Marchant https://doi.org/10.1007/BF00140197
  11. J. Soc. Dyes Colourists v.112 Environmentally friendly, productive and reliable : priorities for cotton dyes and dyeing processes Phillips, D
  12. Colour Chemistry : Synthesis, Properties and Applications of Organic Dyes and Pigments Zollinger, H