Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Biological Characteristics of Tetrastichus sp. reared on Artificial Host

인공숙주에서 증식된 Tetrastichus sp.의 생물학적 특성

  • 이장훈 (동국대학교 자연과학연구원) ;
  • 이기상 (주식회사 쎄실, 종합방제팀) ;
  • 이해풍 (동국대학교 생물학과)
  • Published : 2002.06.01
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Abstract

A gregarious pupal endoparasitoid Tetrastichsus sp. (Hymenoptera: Eulophidae) was reared in vitro from oviposition to adult emergence on an artificial host. The host pupal case was made using 0.02 mm-thick polypropylene film, and was filled with a diet consisting of powders of Antheraea pernyi pupa, chicken yolk, infant formula, royal jelly, and Neisenheimer's salt solution. Female parasitoids reared in the artificial host produced smaller sized progeny than those reared in in vivo, but the adults reproduced fertile offsprings. Furthermore in vitro second-generation (G$_2$) females showed more improved biological characteristics, compared with their parents. The fecundity (mean no. adult progeny), oviposition period (days), and longevity (days) of G$_2$ female were evaluated as 45.7, 7.8, and 13.8, respectively Female biased sex ratio was obtained with 76.9% female progeny. The results demonstrated that Tetrastichus sp. is a promising parasitoid for in vitro mass production.

Tetrastichus sp.는 미국흰불나방의 번데기 기생벌로서, 1개의 숙주에 여러마리의 자손을 번식하는 집단 기생벌이며, 일년에 여러 세대 발생한다. 작잠, 신생아용 분유, 난황, 로얄젤리 등을 주요성분으로 기생벌의 인공먹이를 조성하였으며, polypropylene필름으로 번데기형태를 만들어 인공숙주로 사용하였다. 인공숙주에서 발육한 기생벌은 자연숙주에서 자란 기생벌보다는 크기가 작았으나, 계대 사육으로 번식 가능한 자손을 생산하였다. 인공숙주에서 발육한 in vitro 2세대의 생물학적 특성은 in vitro 1세대보다 훨씬 개선 었다. 평균 자손 수, 산란기간, 수명 등은 in vitro 에서 45.7,7.8, 13.8일 이었고 암컷 편중의 성비 76.9%를 나타내었다. 이러한 결과는 Tetrastichus sp.가 in vitro 대량사육에 적합한 기생벌임을 보여준다.

Keywords

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