The Korean Journal of Food And Nutrition (한국식품영양학회지)

The Korean Society of Food and Nutrition (한국식품영양학회)
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Biosynthetic Regulation of Invertase from Thermophilic and Alkalophilic Bacillus sp. TA-11

고온성이며 호알칼리성인 Bacillus sp. TA-11이 생성하는 Invertase의 생합성 조절

  • Kim, Jae-Ho (Dept. of Genetic Engineering, Paichai University) ;
  • Kim, Na-Mi (Division of Product Development, Korea Tabacco & Ginseng Company Central Institute) ;
  • Kim, Dong-Woo (Division of Food Science and Technology, Woosong Technical College)
  • Published : 2002.06.01
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Abstract

Regulation of invertase biosynthesis was studied in thermophilic and alkalophilic Bacillus sp. TA-11. Biosynthesis of the invertase was effectively induced in the presence of 10 mM sucrose for 180 min. Glucose repressed the invertase induction by sucrose and as late as addition time of glucose, the invertase formation was increased, indicating that glucose repression was occurred by inducer exclusion. Catabolite repression was reduced a little by the addition of cAMP for 180 min of induction.

고온성이며 호알칼리성인 Bacillus sp. TA-11이 생성하는 Invertase의 생합성 조절 기작을 규명하고자 먼저 이들의 유도와 억제에 관하여 검토하였다. Invertase는 10mM sucrose을 함유한 생합성 조절배지에서 3시간에 효율적으로 유도되었고 glucose는 sucrose에 의한 invertase 유도를 inducer exclusion 방식으로 억제시켰다. CAMP의 첨가로 glucose에 의한 catabolic repression이 다소 줄어들었다.

Keywords

References

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