A Study on Antioxidant System in Cataract Patients

한국인 백내장환자의 항산화 체계에 관한 연구

  • 고영숙 (연세대학교 식품영양학과) ;
  • 홍영재 (연세대학교 의과대학 안과학교실) ;
  • 정혜연 (연세대학교 식품영양학과) ;
  • 김수연 (연세대학교 식품영양학과) ;
  • 이양자 (연세대학교 식품영양학과)
  • Published : 2002.03.01

Abstract

Cataracts are the leading cause of blindness worldwide and are characterized by increased opacity of the lens that significantly diminishes visual acuity. It has been suggested that increased risk of lens opacities are associated with age, exposure to sunlight, diabetes, smoking, and poor nutrition. Antioxidant nutrients have born demonstrated to protect the lens membrane and protein against damage due to oxidative stress. The purpose of this study was to investigate the antioxidant system in the blood of cataract patients. The status of the blood antioxidant system was evaluated based on the levels of antioxidant vitamins and minerals as well as glutathione peroxidase (GSH-Px) and malondialdehyde (M7A) activity in 34 patients with cataracts (17 male and 17 female) and 45 control subjects (20 male and 25 female). After adjusting for age, the results showed significantly lower levels of antioxidant vitamins such as lycopene (M : p < 0.05, F: p < 0.01), zeaxanthin (F: p < 0.01), ${\gamma}$-tocopherol (F: p < 0.01) and ascorbic arid (M: p < 0.05) in the cataract patients than in the control subjects. In contrast, the concentration of cryptoxanthin (F : p < 0.07) showed a significantly higher value in the cataract patients. The serum level of the antioxidant mineral Zn (M : p < 0.01) was found to be significantly lower in the cataract patients while the ratio of cu/zn appeared significantly higher (M : p < 0.05). Significantly higher (M : p < 0.01, F: p < 0.05) concentrations of MDA in serum was found in the cataract patients as compared to the control subjects. GSH-Px activity was significantly lower (F: p < 0.05) in 71e cataract patients. In conclusion, the antioxidant system may play an important roll in cataract creation. Further studies are needed to clarify the mechanisms underlying these findings and to establish preventive measures with an emphasis on antioxidant nutrition for cataract patients.

Keywords

References

  1. Annals Epidemiol v.6 Antioxidants and agerelated eye disease;current and future perspectives Christen WG;Glynn RJ;Hennekens CH https://doi.org/10.1016/1047-2797(95)00094-1
  2. Br J Nutr v.80 no.S Functional food science and defence against reactive oxidative species Diplock AT;Charleux JL;Crozier-Willi G;Kok FJ;Rice EC;Roberfriod M;Stahl W;Vina-Ribers J https://doi.org/10.1079/BJN19980106
  3. Ophthalmol v.106 Associations of antioxidant enzymes with catarat and age-related macular degeneration Delcourt C;Cristol JP;Leger CL https://doi.org/10.1016/S0161-6420(99)90059-3
  4. Am J Med v.97 no.S Antioxidants and eye desease Christen WG
  5. Nutr Rev v.51 Antioxidant nutrition and cataract in women;A prospective study Bunce GE https://doi.org/10.1111/j.1753-4887.1993.tb03076.x
  6. Surv Ophthalmol v.39 Epidemiology of risk factors for age-related cataract West SK;Valmadrid CT https://doi.org/10.1016/S0039-6257(05)80110-9
  7. J Kor Ophthalmol Soc v.34 The clinical survey on the prevalence of eye deseases in eldery patients in Sungnam-Shi area Kim KB;Pack JC;Song BR;Koo BS
  8. J Kor Ophthalmol Soc v.31 Morphological classification of the lens opacity of age-related cataract in one area Kim IS;Kim WS;Lee YO;Kim JC;Shyn KH
  9. J Kor Ophthalmol Soc v.17 Retrospective sbservations of cataract sugery patients Ahn CS
  10. J Kor Ophthalmol Soc v.24 A clinicostatistical analysis of senile cataract Kim KH
  11. J Kor Ophthalmol Soc v.39 The change of blindness-causes in Korea Ahn JE;Seo KY;Kho HJ;Kim SH;Kwon OW;Koo BS;Doo SJ;Lee HK
  12. Am J Ophthalmol v.128 The epidemiology of cataract in Australia McCarty CA;Mukesh BN;Fu CL;Taylor HR https://doi.org/10.1016/S0002-9394(99)00218-4
  13. Invest Ophthalmol and Visual Sci v.39 Nuclear cataracts and nutrition;hope for intervention early and late in life Beebe DC
  14. Am J Clin Nutr v.62 no.S Relation among aging, antioxidant status, and cataract Taylor A;Jacques PF;Epstein EM
  15. Arch Biochem & Biophys v.351 The aggregation in human lens proteins blocks the scavenging of UVA-generated singlet oxygen by ascorbic acid and glutathione Lenetsky M;Ranson N;Ortwerth BJ https://doi.org/10.1006/abbi.1997.0548
  16. J Biol Chem v.273 The hydroxyl radical in lens nuclear cataractogenesis Fu S;Dean R;Southan M;Truscott R https://doi.org/10.1074/jbc.273.44.28603
  17. Annals Pharmacotherapy v.28 Antioxidants may retard cataract formation Floren LC;Schroeder DJ;Bereskin AC
  18. Arch Biochem & Biophys v.321 Similar metabolites formed from β-carotene by human gastric mucosal homogentes, lipoxygenase or linoleic acid hydroperoxide Yeum KJ;Lee-Kim YC;Yoon S;Lee KY;Pack IS;Lee KS;Kim BS;Tang G;Russell RM;Krinsky NI https://doi.org/10.1006/abbi.1995.1382
  19. HPLC in Food Analysis(2nd ed.) Macrae R
  20. Clin Chem v.32 Sampling and analysis techniques for monitiring serum for trace elements Scott PE;Michael LM;Barrett ER;Kurt GK;Carmen SA;Joseph AW;Sharon WA
  21. J Lab & Clin Med v.70 Studies on the quantiative and qualitative characterization of erythrocyte glutathione peroxidase Paglia DE;Valentine WN
  22. J Nutr v.117 Effects of dietary selenite, selenocysteine and selenocethionine on selenocysteine lyase and glutathione peroxidase activities and on selenium levels in rat tissues Deagen JT;Butler JA;Beilstein MA;Wharyer PD
  23. J Nutr v.115 Effect of dietary polyunsaturated/saturated fatty acid ratio and dietary vitamin E on lipid peroxidation in the rat Buckingham KW
  24. Am J Clin Nutr v.53 no.S Epidemiologic evidence of a role for the antixidant vitamin and carotenoids in cataract prevention Jacques PF;Chylack LT Jr
  25. Curr Eye Res v.19 Fat-soluble nutrient concentrations in different layers of human cataractous lens Yeum KJ;Shang F;Schalch W;Russell RM;Taylor A https://doi.org/10.1076/ceyr.19.6.502.5282
  26. Eye v.12 Nutrition supplement and the eye Phelps Brown NA;Bron AJ;Harding JJ;Dewar HM https://doi.org/10.1038/eye.1998.21
  27. Am J Med v.97 no.S Reactive oxygen species and antioxidant vitamins;mechanisms of action Frei B
  28. J Am Coll Nutr v.19 no.S The potential role of dietary xanthophylls in cataract and age-related macular degeneration Moeller SM;Jacques PF;Blumberg JB https://doi.org/10.1080/07315724.2000.10718975
  29. Br Med J v.305 Serum antioxidant vitamins and risk of cataract Knekt P;Heliovaara M;Rissanen A;Aromaa A;Aran R https://doi.org/10.1136/bmj.305.6866.1392
  30. Intern J Vit & Nutr Res v.66 Quantitation of vitamin E and a carotenoid pigment in cataractous human lenes, and the effect of a dietary supplement Bates CJ;Chem SJ;Macdonaid A;Holden R
  31. Studies on the status of glutamate and antioxidant system in patients with retinal ganglion cell damage Chung HY
  32. Am J Epidemiol v.149 Antioxidant intake and risk of incident age-related nuclear cataracts in the Beaver Dam Eye Study Lyle BJ;Mares-Perlman JA;Klein BEK;Klein R;Greger JL https://doi.org/10.1093/oxfordjournals.aje.a009895
  33. Epidemiol v.4 Plasma antioxidant and risk of cortical and nuclear cataract Vitale S;West SK;Hallfrisch J;Alstob C;Wang F;Moorman C https://doi.org/10.1097/00001648-199305000-00003
  34. Am J Clin Nutr v.69 Serum carotenoids and tocopherols and incidence of age-related nuclear cataract Lyle BJ;Mares-Perlman JA;Klein BEK;Klein R;Palta M;Bowen PE;Greger JL
  35. J Am Diet Assoc v.86 Vitamin E in foods form high and low linoleic acid diets Lehmann J;Martin HL;Lashley EL
  36. Nutr Rev v.55 r-tocopherol;An efficient protector of lipids against nitric oxide-initiated peroxidative damage Wolf G https://doi.org/10.1111/j.1753-4887.1997.tb01566.x
  37. Vis Res v.38 Water soluble antioxidants in mammalian aqueous humor;interaction with UV B and hydrogen peroxide Richer SP;Rose RC https://doi.org/10.1016/S0042-6989(98)00069-8
  38. J Kor Ophthalmol Soc v.32 Chemical analysis of aqueous humor in cataract and glaucoma patients Hong YJ;Yun YS;Lee YG;Kim CY
  39. Curr Eye Res v.10 Relationship in humans between ascorbic acid consumption and levels of total and reduced ascorbic acids in lens, aqueous humor and plasma Taylor A;Jacques PF;Nadler D;Morrow F;Sulksy SI;Shepard D https://doi.org/10.3109/02713689109013869
  40. Am J Clin Nutr v.66 Long-term vitamin C supplement use and prevalence of early age-related lens opacities Jacques PF;Taylor A;Hankinson SE;Willett WC;Mahnken B;Lee YH;Vaid K;Lahav M
  41. Am J Clin Nutr v.53 no.S Epidemiologic evidence of a role for the antioxidant vitamins and carotenoids in cataract prevention Jacques PF;Chylack LT Jr
  42. J Clin Epidemiol v.52 Serum ascorbic acid and other correlates of self-reported cataract among older Americans Simon JA;Hudes ES https://doi.org/10.1016/S0895-4356(99)00110-9
  43. J Nutr v.110 Relationship between dietary intake and tissue levels of reduced and total vitimin C in the guinea pig Berger J;Shepard D;Morrow F;Yaylor A
  44. Am J Clin Nutr v.53 Antioxidant nutrients and disease prevention: an overview Kiplock AT
  45. Modern Nutrition in Health and Disease(8th ed.) Shils ME;Olson JA;Shike M
  46. Am J Epidemiol v.133 Risk factors for age-related cortical, nuclear, and posterior subcapsular cataracts The Italian-American Cataract Study Group
  47. Nutr Rep Int v.37 Plasma calcium and trace metals in human subjects with mature cataract Bhat KS
  48. Cornell Vet v.72 Differentiation of soluble proteins in cataracts caused by deficiencies of methionine, riboflavin or zinc diets fed to Atlantic salmon, Salmo salar, rainbow trout, Salmo gairdneri, and lake trout, Salvelinus namaycush Barash H;Poston HA;Rumsey GL
  49. Adv Exp Med Biol v.258 Effect of estrogen on serum and tissue levels of copper and zinc Mehta SW;Erkum R
  50. Methods in Enzymol v.233 High-performance liquid chromatography-electrochemical detection of antioxidants in vertebrate lens: glutathione, tocopherol, and ascorbate Mitton KP;Trevithick JR
  51. Exp Eye Res v.68 no.1 Protection from oxidative insult in glutathione depleted lens epithelial cells Reddan JR;Giblin FJ;Kadry R;Leverenz VR;Pena JT;Dziedzic DC https://doi.org/10.1006/exer.1998.0606
  52. J Biol Chem v.252 Speroxide dismutase;an enzymatic funcition for erythrocuprin (hemocuprein) McCord JM;Fridorich I
  53. Ophthalmic Res v.31 Superoxide dismutase, catalase, glutathione peroxidase and xanthine oxidase in diabetic rat lenses Osman C;Yavuz B;Yuksel T;Omer A;Guler Z https://doi.org/10.1159/000055557
  54. Biomedical & Environ Sci v.9 Antioxidant status in persons with and without senile lens changes Xue An;Cai QY;Wang SQ;Zhou AS;Li WX;Fu P;Chen XS
  55. Toxicol v.139 Effects of some probable antioxidants on selenite-induced cataract formation and oxidative stress-related parameters in rats Orhan H;Marol S;Hepsen IF;Sahin G https://doi.org/10.1016/S0300-483X(99)00128-6