Journal of Nutrition and Health

The Korean Nutrition Society (한국영양학회)
권호 표지

The Effect of Conjugated Linoleic Acid and Its Isomers on the Proliferation of Prostate TSU-Prl Cancer Cells

Conjugated Linoleic Acid (CLA)와 그 이성체가 전립선 암세포의 증식에 미치는 영향

  • 오윤신 (한림대학교 생명과학부 식품영양전공) ;
  • 김은지 (한림대학교 생명과학부 식품영양전공) ;
  • 김종우 (한림대학교 비뇨기과) ;
  • 김우경 (단국대학교 식품영양학과) ;
  • 이현숙 (한림대학교 생명과학부 식품영양전공) ;
  • 윤정한 (한림대학교 생명과학부 식품영양전공)
  • Published : 2002.03.01
Download PDF
⟨ Previous Next ⟩

Abstract

Conjugated linoleic acid (CLA) is a collective term for positional and geometric isomers of octadecadienoic acid in which the double bonds are conjugated. CLA has anticancer activity in a variety of animal cancer models, and cis-9, trans-11 (c9t11) and trans-10, cis-12(t10c12) CLA are the most predominant isomers present in the synthetic preparations utilized in these animal studies. To compare the ability of c9t11, t10c12 and an isomeric mixture of CLA to inhibit TSU-Prl cell growth, cells were incubated in a serum-free medium with various concentrations of these fatty acids. The isomeric mixture inhibited cell growth in a dose-dependent manner (1-3 $\mu$M) with a 41 $\pm$ 1% inhibition observed at 3 $\mu$M concentration after 48 hours. T10c12 also inhibited cell proliferation in a dote-dependent manner, However, the efficacy and potency of this isomer was much greater than that of the isomeric mixture with a 49 $\pm$ 2% inhibition observed at 0.3 $\mu$M concentration after 48 hours. By contrast, c9t11 slightly increased cell proliferation. To determine whether the growth-inhibiting effect of CLA is related to the changes in production of insulin-like growth factors (IGF) and IGF-binding proteins (IGFBP) by these cells, serum-free conditioned media were collected. Immunoblot analysis of conditioned media using a monoclonal anti-IGF-II antibody showed that both the isomeric mixture and t10c12 inhibited secretion of both mature 7,500 Mr and higher Mr forms of pro IGF-II, whereas c9t11 had no effect. Ligand blot analysis with 125I-IGF-II revealed the presence of two types of IGFBPs : 24,000 Mr IGFBP-4 and 30,000 Mr IGFBP-6. The production of IGFBP-4 slightly decreased at the highest concentrations of the isomeric mixture and t10c12. These results indicate that CLA inhibits human prostate cancer cell growth, an effect largely due to the action of t10c12. The growth inhibition may result, at least in part, from decreased production of IGF-II and IGFBP-4 by these cells.

Keywords

References

  1. Cambell's Urology(7th ed.) The natural history of localized prostate cancer: a guide to therapy Walsh PC;Walsh PC(ed.);Retik AB(ed.);Vaughan ED Jr(ed.);Wein AJ(ed.)
  2. Cancer Res v.52 The lessons of life;keynote address to the nutrition and cancer conference Doll R
  3. J Natl Cancer Inst v.66 The cause of cancer;quantitative estimates of avoidable risks of cancer in the United States today Doll R;Peto R
  4. Prostate v.24 Regulation of DU145 human prostate cancer cell proliferation by insulin-like growth factors and its interaction with the epidermal growth factor autocrine loop Rose DP;Connolly JM https://doi.org/10.1002/pros.2990240402
  5. Carconogenesis v.8 Anticarcinogens from fried ground beef;heat-altered derivatives of linoleic acid Ha YL;Grimm NK;Pariza MW
  6. Cancer Res v.50 Inhibition of benzo (a)pyrene indeced mouse forestomach neoplasia by conjugated dienoic derivatives of linoleic acid Chin SF;Storkson J;Pariza MW
  7. Proc Soc Exp Biol Med v.223 Mechanisms of action of conjugated linoleic acid;evidence and speculation Pariza MW;Park Y;Cook ME https://doi.org/10.1046/j.1525-1373.2000.22302.x
  8. J Am Oil Chem Soc v.50 Lipid composition of selected margarines Carpenter DL;Slover HT https://doi.org/10.1007/BF02640843
  9. Cancer Lett v.150 Reduction of murine mammary tumor metastasis by conjugated linoleic acid Hubbard NE;LIm D;Summers L;Erickson KL https://doi.org/10.1016/S0304-3835(99)00379-1
  10. Anticancer Res v.19 Opposite effect of linoleic acid and conjugated linoleic acid on human prostatic cancer in SCID mice Cesano A;Visonneau S;Scimeca JA;Kritchevsky D;Santoli D
  11. Cancer Res v.51 Mammary cancer prevention by conjugated dienoic derivative of linoleic acid Ip C;Chin SF;Schmeca Joseph A;Pariza MW
  12. Endocrine v.127 The possible autocrine/paracrine and endocrine roles of insulin-like growth factors of human tumors Daughday WH https://doi.org/10.1210/endo-127-1-1
  13. Endocr Rev v.16 Insulin-like growth factors and their binding proteins;biological actions Jones JI;Clemmons DR
  14. Am J Physiol Endocrinol Metab v.278 Insulin-like growth factor(IGF)-binding proteins;interactions with IGFs and intrinsic bioactivities Baxter RC
  15. Endocr Rev v.20 The insulin-like growth factor-biding protein (IGFBP) superfamily Hwa V;Oh T;Rosenfeld RG https://doi.org/10.1210/er.20.6.761
  16. Rec Prog Horm Res v.46 Insulin-like growth factor binding proteins Rosenfeld RG;Lamson G;Pham H;Conover C;De Leon DD;Domovan SM;Ocrant I;Ciudice L
  17. Breast Cancer Res Treat v.47 IGF-independent regulation of breast cancer growth by IGF binding proteins Oh Y https://doi.org/10.1023/A:1005911319432
  18. Br J Cancer v.85 Insulin-like growth factor- Ⅰand prostate cancer;a meta-analysis Shi R;Berkel HJ;Yu H https://doi.org/10.1054/bjoc.2001.1961
  19. J Clin Endocrinol Metab v.73 Insulin-like growth factors, IGF receptors, and IGF binding porteins in primary cultures of prostate epithelial cells Cohen P;Peehl DM;Lamson G;Rosenfeld RG https://doi.org/10.1210/jcem-73-2-401
  20. Prostate v.22 Insulin-like growth factor-1-action and receptor characterization in human prostate cancer cell lines Iwamura M;Sluss PM;Casamento JB;Cockett AT https://doi.org/10.1002/pros.2990220307
  21. J Urol v.137 Establishment of a new prostatic carcinoma cell line(TSU-Pr1) Iizumi T;Yazaki T;Kanoh S;Kondo I;Koiso K https://doi.org/10.1016/S0022-5347(17)44488-0
  22. J Immunological Method v.89 Rapid colorimetric assay for cell growth and survival modification to the tetrazolium dye procedure giving improved sensitivity and reliability Denizot F;Lang R https://doi.org/10.1016/0022-1759(86)90368-6
  23. Endocrinology v.134 Direct detection of insulin-like growth factorⅡ(IGF-Ⅱ) by chemiluminescene without interference by IGF binding proteins De Leon DD;Terr C;Nissley P https://doi.org/10.1210/en.134.4.1960
  24. Endocrinology v.124 Identification of a family of insulin-like growth factor Ⅱ secreted by cultured rat epithelial-like cell line 48.54-SF;application of a monoclonal antibody Tanaka H;Asami O;Hayano T;Sasaki I;Yoshitake Y;Nishikawa K https://doi.org/10.1210/endo-124-2-870
  25. Eur J Biochem v.170 Heterogeneity of insulin-like growth factor binding preteins between structure and affinity.1. Circulating forms in man Hossenlopp P;Segovia-Quinson B;Portolan G;Binoux M https://doi.org/10.1111/j.1432-1033.1987.tb13677.x
  26. Principles and Procedures of Statistics(2nd ed.) Steel RGD;Torrie JH
  27. Exp Cell Res v.250 Conjugated linoleic acid inhigits proliferation and induces apoptosis of normal rat mammary epithelial cells in primary culture Ip MM;Masso-Welch PA;Shoemaker SF;Shea-Eaton WK;Ip C https://doi.org/10.1006/excr.1999.4499
  28. Adv Exp Med Biol v.289 Formation and action of anticarcinogenic fatty acids Pariza MW;Ha YL;Benjamin H;Sword JT;Gruter A;Chin SF;Storkson;Faith N;Albright K https://doi.org/10.1007/978-1-4899-2626-5_19
  29. Cancer Res v.50 Inhibition of benzo[a]pyrene-induced mouse forestomach neoplasia by conjugated dieno derivatives of linoleic acid Ha YL;Storkson J;Parizza MW
  30. Biochem Biophys Res Commun v.287 Trans-10, cis-12-conjugated linoleic acid reduced leptin secretion from 3T3-L1 adipocytes Kang K;Pariza MW https://doi.org/10.1006/bbrc.2001.5603
  31. Cell v.79 Oncogenes and the strategy of growth factors Baserga R https://doi.org/10.1016/0092-8674(94)90023-X
  32. Cancer Res v.55 The insulin-like growth factorⅠ receptor;a key to tumor growth? Baserga R
  33. Adv Cancer Res v.68 The role of the insulin-like growth factor system in human cancer Werner H;LeRoith D https://doi.org/10.1016/S0065-230X(08)60354-1
  34. World J Urol v.19 Insulin-like growth factors and prostate cancer Djavan B;Waldert M;Seitz C;Margerger M https://doi.org/10.1007/s003450100220
  35. J Clin Endocrinol Metab v.82 Growth hormone/insulin-like factor-Ⅰaxis in aging;a summary of an NIA sponsored symposium Rosen CJ;Conover C
  36. BJU Int v.87 Are dietary influences on the risk of prostate cancer mediated through the insulin-like growth factor system? Mucci LA;Tamimi R;Lagiou P;Trichopoulou A;Benetou V;Spans E;Trichopoulos D https://doi.org/10.1046/j.1464-410x.2001.02191.x
  37. Cell tissue Res v.291 Expression of insulin-like growth factor (IGF)-Ⅱ in human prostate, breast, bladder, and paraganglioma tumors Li SL;goko H;Xu ZD;Kimura G;Sun Y;Kawachi MH;Wilson TG;Wilczynski S;Fujita Yamaguchi Y
  38. Int J Urol v.3 Insulin-like growth factor (IGF) system components in human prostatic cancer cell-line;LNCaP, Du-145, and PC-3 cells Kumura G;Kasuya J;Giannini S;Honda Y;Mohan S;Kawachi Mark H:Akimoto M;Fujita-Yamaguchi Y https://doi.org/10.1111/j.1442-2042.1996.tb00628.x
  39. J Clin Endocrinol Metab v.77 Serum insulin-like growth factor-binding protein-2 (IGFBP-2) is increased and IGFBP-3 is decreased in patients with prostate cancer;Correlation with serum prostate-specific antigen Kanety H;Madjar Y;Dagan Y;Levi J;Papa MZ;Pariente C;Golewasser B;Karasik A https://doi.org/10.1210/jc.77.1.229
  40. Growth Regul v.3 IGF binding protein-3 secreted by the prostate adenocarcinoma cells (PC-3);differentiation effect on PC-3 and normal prostate cell growth Kacier EK;Blat C;Imbenotte J;Troalen F;Cussenot O;Calve F;Harel L
  41. Endocrinoligy v.142 Inhibition of growth and increased expression of insulin-like growth factor-binding protein-3 (IGFB-3) and -6 in prostate cancer cells stably transfected with antisense IGFBP-3 complementary deoxyribonucleic acid Drivdahl RH;Sprenger C;Trimm K;Plymate SR https://doi.org/10.1210/en.142.5.1990