Polyclonal Antibody against Paenibacillus larvae and its Application

Paenibacillus larvae에 대한 다클론 항체 및 그 응용

  • 백경찬 (경기대학교 자연과학부 생물학전공) ;
  • 양옥순 (경기대학교 자연과학부 생물학전공) ;
  • 정규회 (경기대학교 자연과학부 생물학전공) ;
  • 윤병수 (경기대학교 자연과학부 생물학전공)
  • Published : 2002.03.01

Abstract

Paenibacillus larvae is a gram-positive, spore-forming bacterium that is etiological agent for american foulbrood disease (AFB), which is the most severe disease in honey bee. To detect P. larvae from infected honeybee-comb or larvae, polyclonal antibody against whole bacterium was produced from guineapig and its specificity was evaluated. After optimization of ELISA-based detection system using these antibodies, a number of different P. larvae strains were analysed. Polyclonal antibody against P. larvae ATCC 25747 showed high affinity to most strains of P. larvae including P. larvae. strain ATCC 9545 (type strain), ATCC 25747 and other korean strain, SJl5 but exhibited no cross-reaction with other bacterial species. Additionally, this type of ELISA system was used for the detection of AFB in field-application The results have shown that this antibody could be useful for the rapid identification and monitoring of P. larvae in honeybee-comb.

Paenibacillus larvae는 Gram양성의 내생포자 형성 세균으로, 꿀벌의 질병 중 가장 심각한 피해를 입히는 미국부저병(AFB)의 원인균이다. 이 P. larvae를 발병전인 봉군 또는 애벌레에서 보다 빠르게 검출하기 위하여 기니아피그를 이용하여 이 병원균에 대한 다클론 항체를 제작하였고 또한 그 항체의 성능을 평가하였다 제작된 항체를 이용한 ELISA검색법을 개발한 후, 다수의 P. larvae균주를 검사하였다. 그 결과 P. larvae ATCC 25748의 균체를 사용하여 제작된 항체는 ATCC 9545 (대표균주), ATCC 25747, 국내 분리 균주인 SJl5등과 높은 항체 친화성을 나타내었으며. 다른 세균 종들과는 반응하지 아니하였다. 또한 이 ELISA검색법은 미국 부저병의 현장검사에 적용하기 위하여 사용되었으며, 그 결과로 본 연구에서 제시하는 항체는 벌집 내 또는 애벌레 내에 존재하는 P. larvae의 빠른 동정과 모니터 링에 매우 유용함을 보여주었다.

Keywords

References

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