Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
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Analyses of Genetic Relationships of Collectorichum spp. Isolated from Sweet Persimon with RAPD and PCR-RFLP.

단감나무로부터 분리한 탄저병 병원균 Colletotrichum spp.의 RAPD와 PCR-RFLP를 이용한 유연관계 분석

  • 김희종 (농우 바이오, 강원대학교 농업생명과학대학 식물응용과학부) ;
  • 엄승희 (농우 바이오, 강원대학교 농업생명과학대학 식물응용과학부) ;
  • 이윤수 (농우 바이오, 강원대학교 농업생명과학대학 식물응용과학부)
  • Published : 2002.03.01
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Abstract

Colletotrichum species are important fungal pathogen that cause great damages on various host plant species worldwide. In Korea, Colletotrichum species cause massive economic losses on apple, peach, grape, and essecially, sweet persimon productions. In the past, Identification of the pathogen and the studies on the genetic relationships among the pathogenic isolates were mainly based on morphology, cultural characteristics, and the difference in pathogenicity. However, in recent years, these traditional methods have been replaced with molecular methods to solve the difficulty of classification on pathogens. Therefore, in this study, RAPD and PCR-RFLP methods were employed for the studies of genetic relationship among the different isolates of Colletotrichum species that cause damages on sweet persimon. As a results of genetic relationship analysis, Colletotrichum species tested were divided into two big groups or five small groups.

Colletotrichum spp.는 광범위한 기주범위를 갖는 다범성균으로 각종작물에 피해를주는 중요한식물병원진균이다. 최근 국내에서 널리 재배되고 있는 단감, 사과, 복숭아, 포도 등에 탄저병 이 발생하여 많은 경제적 손실을 초래하고 있다. 탄저병원균의 경우 기존에는 주로 형태적 특징이나 배지 상에서의 특성, 기주에 대한 병원성의 차이에 의존하여 분류를 해 왔다. 그러나 최근에는 병원균의 분류에 있어 문제점을 해결하기 위하여 분자생물학적 방법을 이용하고 있다. 이에 본 실험에서는 Random Amplified Polymorphism DNAs (RAPD)와 Ploymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) 기법을 이용하여 단감나무에 탄저병을 일으키는 균들 간에 유연관계를 밝혔다. 유연관계 분석결과 크게는2개의 그룹으로 나뉘었고 작게는5개의 그룹으로 나뉘는 것을 알 수 있었다.

Keywords

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