Dynamics of Microbial Community of Aerobic Biofilm Reactor using Rhodococcus sp. EL-GT and Activated Sludge for Phenol Wastewater Treatment

페놀폐수 처리를 위한 Rhodococcus sp. EL-GT와 활성슬러지를 이용한 호기성 생물막 반응기의 미생물 군집 동태

  • Published : 2001.06.01

Abstract

This research was performed to investigate the dynamics of microbial community by RBC (Rotating Biological Contactor) using Rhodococcus sp. EL-GT and activated sludge. Cell counts revealed by DAPI were compared with culturable bacterial counts from nutrient agar. Colony counts on nutrient agar gave values 20~25% and 1~15% of cell counts (DAPI). The cell counts for the dynamics of bacterial community were determined by combination of in situ hybridization with fluorescently-labelled oligonyucleotide probes and epifluorescence microscopy. Around 90~80% of total cells visualized DAPI were also detected by the bacteria probe EUB 338. For both reactors proteobacteria belonging to the gamma subclass were dominant in the first stage (1 and 2 stage) and proteobacteria belonging to the gamma subclass were dominant in the last stage (3 and 4 stage).

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References

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