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참박과 호박 종자의 발아촉진을 위한 저선량 감마선의 효과

Effect of Low-Dose Gamma Radiation to Enhance Germination Rate in Bottle Gourd and Pumpkin Seeds

  • 김재성 (한국원자력연구소 동위원소 방사선응용연구팀) ;
  • 백명화 (한국원자력연구소 동위원소 방사선응용연구팀) ;
  • 이영근 (한국원자력연구소 동위원소 방사선응용연구팀) ;
  • 이혜연 (한국원자력연구소 동위원소 방사선응용연구팀) ;
  • 유준철 (한국원자력연구소 동위원소 방사선응용연구팀)
  • 발행 : 2002.09.30

초록

참박과 호박 채종종자의 발아율을 증진시킬 수 있는 종자처리 방법을 확립하고자 저선량 감마선을 1$\sim$20 Gy 까지 조사하여 발아와 초기생육 및 생리활성을 관찰하였다. 저선량 조사한 참박과 호박종자의 발아율은 대조구보다 증가하는 경향을 보였는데 그 효과는 종자상태에 따라 다르게 나타났다. 참박 70$\sim$80% 발아율종자의 발아는 4$\sim$20 Gy에서 증가하였는데 특히 4 Gy조사에서 가장 높았고, 80$\sim$90% 발아율종자도 4 Gy 조사에서 효과를 보였다. 파종 4일 후의 발아율은 참박 70$\sim$80%와 80$\sim$90% 발아율 종자의 경우는 4 Gy조사에서 대조구보다 각각 66%와 26% 증가하였고, 호박의 70$\sim$80% 발아율종자는 2 Gy 조사에서, 80$\sim$90% 발아율종자는 8 Gy 조사에서 향상되었다. 저선량 조사한 유묘생육은 발아율이 증가한 선량에서 증가하였는데 특히 호박 70$\sim$80% 종자의 2 Gy 조사에서 가장 높았다. 파종 6일 후에 조사한 참박의 효소활성은 4 Gy 조사시에 단백질과 catalase 활성이, 호박의 경우는 catalase와 peroxidase 활성이 2 Gy와 8 Gy 조사에서 증가하였다. 참박과 호박종자의 발아와 유묘생육 및 생리활성은 저선량 감마선 조사에 의해 촉진됨을 확인할 수 있었다.

To establish the seed treatment method of the gamma radiation for germination enhancement of bottle gourd(Lagenaria siceraria Standl.) and pumpkin(Cucurbita ficifolia Bouche), seeds were irradiated with the dose of 1$\sim$20 Gy of the gamma radiation. The germination rate in irradiated group was higher than that of the control, depending on seed condition. In the case of bottle gourd, the germination rate of 70$\sim$80% germinative seed was increased at 4$\sim$20 Gy irradiated groups. Especially, it was the highest at 4 Gy irradiated group. The germination rate of 80$\sim$90% germinative seed was the highest at 4 Gy irradiated group. The germination rate of 70$\sim$80% and 80$\sim$90% germinative seed at 4 days after sowing was increased 66% and 26% at 4 Gy irradiated group, respectively. In the case of pumpkin, the germination rate of 70$\sim$80% and 80$\sim$90% germinative seed was increased at 2 Gy and 8 Gy irradiated group, respectively. Effect of the low-dose gamma radiation on the early growth was enhanced depending on their germination enhancement. Especially, it was the highest at 2 Gy irradiated group of 70$\sim$80% germinative seed of pumpkin. Catalase activity of bottle gourd was increased at 4 Gy irradiated group. Catalase and peroxidase activity of pumpkin were increased at 2 Gy and 8 Gy irradiated group. These results suggest that the germination, early growth and physiological activity of bottle gourd and pumpkin seeds could be promoted by the low dose gamma radiation.

키워드

참고문헌

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피인용 문헌

  1. Alterations in the photosynthetic pigments and antioxidant machineries of red pepper (Capsicum annuum L.) seedlings from gamma-irradiated seeds vol.47, pp.4, 2004, https://doi.org/10.1007/BF03030546
  2. Physiological effects of glycinebetaine on gamma-irradiated stressed fenugreek plants vol.33, pp.4, 2011, https://doi.org/10.1007/s11738-010-0641-4
  3. Physiological Effects of Glycinebetaine on Gamma-Irradiated Fenugreek Plants vol.17, pp.1, 2010, https://doi.org/10.1080/19315260.2010.509124
  4. Physiological effects of glycinebetaine on gamma-irradiated stressed fenugreek plants vol.58, pp.2, 2010, https://doi.org/10.1556/AAgr.58.2010.2.1