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A Technique to Quantify the Extent of Postmortem Degradation of Meat Ultrastructure

  • Hwang, I.H. (Cooperative Research Centre for the Cattle and Beef Industries, University of New England Armidale) ;
  • Thompson, J.M. (Cooperative Research Centre for the Cattle and Beef Industries, University of New England Armidale)
  • Received : 2001.04.11
  • Accepted : 2001.08.25
  • Published : 2002.01.01

Abstract

This study investigated quantitative changes in the spaces between and within myofibrils and the impact of high and low voltage electrical stimulation on muscle ultrastructure as seen in electron micrographs. In addition, the relationships of these spaces and the impact to meat tenderness were investigated. The degradation of myofibrils during aging appeared to be localized across the muscle fibre. Structural deterioration of muscle fibres was evident 1 day post-mortem, involving the weakening in the lateral integrity of the myofibrils and Z-disc regions. Meat tenderisation, as shown by objective measurements, coincided with these increases in degradation, as assessed by the sum of the gaps between and within myofibrils. The results showed that the total size of gaps between and within myofibrils can be used as an indicator of meat tenderization during aging, but that ultrastructural alteration in electrically stimulated muscle had little relationship with meat tenderness.

Keywords

References

  1. Abbott, M. T., A. M. Pearson, J. F. Price and G. R. Hooper, G. R. 1977. Ultrastructural changes during autolysis of red and white porcine muscle. J. Food Sci. 42:1185-1188. https://doi.org/10.1111/j.1365-2621.1977.tb14456.x
  2. Bouton, P. E., P. V. Harris and W. R. Shorthose. 1971. Effect of ultimate pH upon the water holding capacity and tenderness of mutton. J. Food Sci. 36:435-439. https://doi.org/10.1111/j.1365-2621.1971.tb06382.x
  3. Bouton, P. E. and P. V. Harris. 1972. A comparison of some objective methods used to assess meat tenderness. J. Food Sci. 37:218-221. https://doi.org/10.1111/j.1365-2621.1972.tb05820.x
  4. Dutson, T. R., G. C. Smith, J. W. Savell and Z. L. Carpenter. 1980. Possible mechanism by which electrical stimulation improves meat tenderness. In: Proceedings 26th European meet Meat Researchers and Workers. pp. 84-87.
  5. Dykstra, M. J. 1993. A Manual of Applied Technologies for Biological Electron Microscopy. Plenum Press, New York.
  6. Geesink, G. H., J. J. M. Van Laack, V. M. H. Barner and F. J. M. Smulders. 1994. Does electrical stimulation affect the speed of aging or aging response? Sciences Des Aliments. 14:409-422.
  7. Goll, D. E., M. L. Boehm, G. H. Geesink and V. F. Thompson. 1997. What causes postmortem tenderization? In: Proceedings 50th Annual Reciprocal Meat Conference, USA. pp. 60-67.
  8. Harris, P. V. and W. R. Shorthose. 1988. Meat texture. In: Developments in Meat Science-4 (Ed. R. A. Lawrie). Elsevier Applied Science Publisher, London. pp. 245-296.
  9. Ho, C. Y., M. H. Stromer and R. M. Robson. 1996. Effect of electrical stimulation on postmortem titin, nebulin, desmin, and troponin T degradation and ultrastructural changes in bovine longissimus muscle. J. Anim. Sci. 74:1563-1575. https://doi.org/10.2527/1996.7471563x
  10. Ho, C. Y., M. H. Stromer, G. Rouse and R. M. Robson. 1997. Effects of electrical stimulation and postmortem storage on changes in titin, nebulin, desmin, troponin-T, and muscle ultrastructure in Bos indicus crossbred cattle. J. Anim. Sci. 75:366-376. https://doi.org/10.2527/1997.752366x
  11. Hwang, I. H. and J. M. Thompson. 2001a. The effect of time and type of electrical stimulation on the calpain system and meat tenderness in beef longissimus dorsi muscle. Meat Sci. 58:134-144. https://doi.org/10.1016/S0309-1740(00)00141-8
  12. Hwang, I. H. and J. M. Thompson. 2001b. The interaction between pH and temperature decline early postmortem on the calpain system and objective tenderness in electrically stimulated beef longissimus dorsi muscle. Meat Sci. 58:167-174. https://doi.org/10.1016/S0309-1740(00)00147-9
  13. Karnovsky, M. J. 1965. A formaldehyde-glutaraldehyde fixative of high osmolality for use in electron microscopy. J. Cell Biol. 27:137-138.
  14. O'Halloran, G. R., D. J. Troy, D. J. Buckley and W. J. Reville. 1997. The role of endogenous proteases in the tenderisation of fast glycolysing muscle. Meat Sci. 47:187-210. https://doi.org/10.1016/S0309-1740(97)00046-6
  15. Olson, D. G., F. C. Parrish Jr. and M. H. Stromer. 1976. Myofibril during postmortem storage. J. Food Sci. 41:1036-1041. https://doi.org/10.1111/j.1365-2621.1976.tb14384.x
  16. Robson, R. M., T. W. Huiatt and F. C. Parrish Jr. 1991. Biochemical and structural properties of titin, nebulin and intermediate filaments in muscle. In: Proceedings 44th Annual Reciprocal Meat Conference, USA. pp. 7-20.
  17. Takahashi, G., S. M. Wang, J. V. Lochner and B. B. Marsh. 1987. Effects of 2-Hz and 60-Hz stimulation on the microstructure of beef. Meat Sci. 19:65-76. https://doi.org/10.1016/0309-1740(87)90100-8
  18. Taylor, R. G., G. H. Geesink, V. F. Thompson, K. Koohmaraie and D. E. Goll. 1995a. Is Z disk degradation responsible for postmortem tenderisation? J. Anim. Sci. 73:1351-1367. https://doi.org/10.2527/1995.7351351x
  19. Taylor, R. G., D. E. Goll and A. Auali. 1995b. Enzyme localization during postmortem muscle tenderization. In: Expression of Tissue Proteinases and Regulation of Protein Degradation as Related to Meat Quality (Ed. A. Ouali, D. I. Demeyer and F. J. M. Smulders). Utrecht. pp. 347-359.
  20. Taylor, R. G. and M. Koohmaraie. 1998. Effects of postmortem storage on the ultrastructure of the endomyosium and myofibrils in normal and callipyge longissimus. J. Anim. Sci. 76:2811-2817. https://doi.org/10.2527/1998.76112811x
  21. Wheeler, T. L., J. W. Savell, H. R. Cross, D. K. Lunt and S. B. Smith. 1990. Mechanisms associated with the variation in tenderness of meat from Brahman and Hereford cattle. J. Anim. Sci. 68:4206-4220. https://doi.org/10.2527/1990.68124206x

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