Asian-Australasian Journal of Animal Sciences

  • 제15권5호
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  • Pages.721-724
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  • 2002
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  • 1011-2367(pISSN)
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  • 1976-5517(eISSN)
아세아태평양축산학회 (Asian Australasian Association of Animal Production Societies)
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Comparison of Postmortem Proteolysis between Breast and Leg Muscles in Chiayi Native Chickens

  • Cha, Shih-Ting (Department of Animal Science, National Chiayi University) ;
  • Tseng, Tsai-Fuh (Department of Animal Science, National Chiayi University) ;
  • Ho, Sy-Shyan (Department of Animal Science, National Chiayi University) ;
  • Chou, Rong-Ghi R. (Department of Animal Science, National Chiayi University)
  • 투고 : 2001.07.24
  • 심사 : 2001.10.24
  • 발행 : 2002.05.01
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초록

Postmortem Proteolysis of breast (BM) and leg (LM) muscles in Chiayi native chickens at $5^{\circ}C$ were compared. Myofibrils were purified from BM and LM samples that were randomly taken from carcasses after 0, 1, 3, 7 and 14 days of storage at $5^{\circ}C$. Fragmentation of myofibrils were determined, and degradation of myofibrillar proteins were analyzed by the SDS-PAGE and western blots. The results showed that myofibril fragmentation index (MFI) was significantly (p<0.05) higher in BM than in LM samples. Disappearance of titin and nebulin and appearance of the 30 kDa component were more rapidly as seen on SDS-PAGE in BM than in LM samples. Western blots labeled with a monoclonal antibody to desmin also demonstrated that desmin degraded more quickly in BM samples. Our data suggested that postmortem proteolysis occurred more rapidly in breast muscles in Chiayi native chickens.

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참고문헌

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