Journal of fish pathology (한국어병학회지)

The Korean Society of Fish Pathology (한국어병학회)
권호 표지

Effects of stress induced by changes of water temperature on the non-specific defense mechanism in cultured olive flounder, Paralichthys olivaceus

수온 변동 자극이 양식 넙치, Paralichthys olivaceus의 비특이적 생체 방어에 미치는 영향

  • 이덕찬 (부경대학교 수산과학연구소) ;
  • 김도형 (부경대학교 대학원 해양산업공학과) ;
  • 김수미 (부경대학교 수산과학연구소) ;
  • 강명석 (부경대학교 산업대학원 수산양식학과) ;
  • 홍미주 (부경대학교 수산생명의학과) ;
  • 김현정 (부경대학교 수산생명의학과) ;
  • 박수일 (부경대학교 수산과학연구소)
  • Published : 2002.08.01
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Abstract

This study was performed to know the effects of stress induced by the daily fluctuation of water temperture from 18$^{\circ}C$ to 25$^{\circ}C$ up and down for 30 days on the defence mechanism of olive flounder. Puralichthys olivaceus. To make clear the temperature stress on the defense mechanism of the tested fish. several factors of immune response such as counting of leucocyte appearance in peripheral blood, phagocytic activity in whole blood cells, nitroblue tetrazolium(NBT) reduction, chemiluminescence(CL) response, and lysozyme activity were investigated at 28 days after giving the change of water temperature. The fish was controlled under the none feeding condition during experimental period. Mortality of the tested fish was rapidly increased up to 22% within the first one week of the experimental period without any additional stress factors. The number of neutrophil of peripheral blood in the tested group was significantly higher than the control group at the 2nd week. but the number of lymphocyte was significantly lower than the control group at the 1st and 3rd day of the experimental period. respectively. In the NBT reduction test, the activity of macrophage in the control group fish was the highest on the 7th day while that in the tested group was on the 3rd day. Also. the phagocytosis of tested group against formalin killed cells was retarded compared with the control. CL response of the tested group was significantly lower from 2nd to 5lh day of the experimental period than the control. 'The lysozyme activity of tested group was remained higher during the experimental period than the control. Even though the tested fish showed different results in some non-specific factors of immune respceses between tested and control group fish, olive flounder seems highly adaptable in repealed water temperature change in condition after one week under the given temperature fluctuation range.

Keywords

References

  1. Angelidis. P., Baudin-Leurencin, F. and Youinou, P. : Stress in rainbow trout. Salmo gairdneri : effects upon phagocyte chemiluminescence. circulating leucocytes and susceptibility to Aeromonas salmonicida. J. Fish Biol., 31 (Supplement A) : 113-122, 1987 https://doi.org/10.1111/j.1095-8649.1987.tb05302.x
  2. Baier-Arderson, C. and Anderson, R. S. : Suppression of superoxide production by chlorothalonil in striped bass (Morone .Saxatilus) macrophage : the role of cellular sulfhydryls and oxidative stress. Aquat. Toxicol., 50 : 85-96, 2000 https://doi.org/10.1016/S0166-445X(99)00092-2
  3. Barton, B. A. and Schreck, C. B. : Influence of acclimation temperature on interrenal and carbohydrate stress responses in juvenile chinook salmon (Oncorhynchus tshawytscha). Aquaculture, 62 : 299-310, 1987 https://doi.org/10.1016/0044-8486(87)90172-4
  4. Brown, J. A., Moore, W. M. and Quabius, E. S. : Physiological effects of saline water on zander. J. Fish Biol., 59 : 1544-1555, 2001 https://doi.org/10.1111/j.1095-8649.2001.tb00219.x
  5. Ellis, A. E. : Stress and the modulation of defence mechanisms in fish. In: Stress and Fish (ed. by Pickering, A. D.), pp. 147-169. Academic Press, London, 1981
  6. Ellis, A. E. : Lysozyme assay. In: Techniques in Fish Immunology. Fish lmmonojogy Technical Communication no.1 (eds. by Stolen, J. S., Fleteher, T. C., Anderson, D. P., Roberson, B. S. and van Muiswinkel, W. B.), pp. 101-103, SOS publications, 1990
  7. Ellsaesser, C. F. and Clem, L. W, : Haematological and immunological changes in channel cat-fish stressed by handling and transport. J. Fish Biol., 28 : 511-521, 1986 https://doi.org/10.1111/j.1095-8649.1986.tb05187.x
  8. Iwama, G. K., Picketing, A. D., Sumpter, J. P. and Schreck. C. B. : Fish Stress and Health in Aquaculture. Cambridge University Press, 1997
  9. Kaattari, S. L. and Tripp, R. A. : Cellular mechanism of glucocorticoid immunosuppression in salmon. J. Fish Biol. (Supplement A), 31 : 129-132, 1987 https://doi.org/10.1111/j.1095-8649.1987.tb05304.x
  10. Maule, A. G. and Schreck C. B. : Stress and cortisol treatment changed affinity and number of glucocorticoid receptors in teukocytes and gill of coho salmon. Gen. Comp. Endocrinol., 84 : 83-93, 1991 https://doi.org/10.1016/0016-6480(74)90144-0
  11. Maule, A. G., Tripp, R. A., Kaattrari, S. L. and Schreck, C. B. : Stress immune function and disease resistance in chinook salmon (Oncorhynchus tshawytscha). J. Endocrinol., 120 : 135-142, 1989 https://doi.org/10.1677/joe.0.1200135
  12. Mazur, C. F. and Iwama, G. K. : Effect of handling and stocking density on hematocrit, plasma cortisol, and survival in wild and hatcheryreared chinook salmon (Oncorhynchus tshawytscha). Aquaculture, 112 : 291-299, 1993a https://doi.org/10.1016/0044-8486(93)90390-K
  13. Mezur, C. f. and Iwama, G. K. : Handling and crowding stress reduces number of plaqueforming cells in Atlantic salmon. J. Aquat. Animal Health, 5 : 98-101, 1993b https://doi.org/10.1577/1548-8667(1993)005<0098:HACSRN>2.3.CO;2
  14. Metcalf, J. A., Gallin, J. I., Nauseef, W. M. and Root, R. K. : Laboratory manual or neutrophil function. pp. 87-143. Raven Press. 1986
  15. Miller, N. W. and Tripp, M. R. : An immunoinhibitory substance in the serum of laboratory held killifish, Fundulus heteroclitus L. J. Fish Biol., 20 : 309-316, 1982 https://doi.org/10.1111/j.1095-8649.1982.tb04712.x
  16. Mock, A. and Peters, G. : Lysozyme activity in rainbow trout, Oncorhynchus mykiss(Walbaum), stressed by handling. transport and water pollution. J. Fish Biol., 37 : 873-885, 1990 https://doi.org/10.1111/j.1095-8649.1990.tb03591.x
  17. Moraes, G. and Bidinotto, P. M. :Metabolic impact of handling on Pseudoplatystoma coruscans, a widespread teleost fish. In: Stress in Fish; Symposium Proceedings (eds. by Barton, B., Pottinger, T., lwama, G. and MacKinlay, D.), pp. 89-100. International congress on the biology of fish, 2000
  18. Peters, G., Faisal, M., Lang, T. and Ahmed, I. : Stress caused by social interaction and its effect on susceptibility 10 Aeromonas hydrophila infection in rainbow trout, Salmo gairdneri. Dis. Aqual. Org., 4 : 83-89, 1988 https://doi.org/10.3354/dao004083
  19. Sautarem, H. M. and Figueras, A. : Leucocyte numbers and phagocytic activity in turbot Scophthalmus maximus following immunization with Vibrio damsela and Pasteurella piscicida O-antigen becerins. Dis. Aquat. Org., 23 : 213-220, 1995 https://doi.org/10.3354/dao023213
  20. Schreck, C. B. : Inununomodulation: endogenous factors. In: The fish immune system: Organism. pathogen, and environment (eds. by Iwama, G. and Nakanishi, T.), pp. 311-337, Academic Press, San Diego, 1996
  21. Scott, A. L. and Klesius, P. H. : Chemilumunescence: A novel analysis of phagocytosis in fish . Dev. Biol. Stand., 49 : 243-254, 1981
  22. Secombes. C. R. : Isolation of salmonid mecrophages and analysis of their killing activity. Tech. Fish lmmunol., pp. 137-154, I990
  23. Smart, G. R. : Aspects of water quality producing stress in intensive fish culture. In: Stress and Fish (ed. by Pickering, A. D.), pp.277-293, Academic Press, London, 1981
  24. Snieszko, S. F.. : The effects of environmental stress on outbreaks of infectious diseases of fishes. J. Fish Biol., 6 : 197-208, 1974 https://doi.org/10.1111/j.1095-8649.1974.tb04537.x
  25. Strange, R. J., Schreck, C. B. and Golden, J. T. : Corticoid stress responses to handling and temperature in salmonids. Trens. Am. Fish. Soc., 106 : 213-217, 1977 https://doi.org/10.1577/1548-8659(1977)106<213:CSRTHA>2.0.CO;2
  26. Stave, J. W., Roberson, B. S. and Hetrick, F. M. : Chemiluminesence of phagocytic cells isolated from the pronephros of striped bass. Dev, Comp. Immunol., 7 : 269-276, 1983 https://doi.org/10.1016/0145-305X(83)90008-3
  27. Takahashi, Y., Itami, T. and Konegawa, K. : Enzymatic properties of partially lysozyme from the skin mucus of carp. Bull. Jap. Soc. Sci. Fish., 52 : 1209-1214, 1986 https://doi.org/10.2331/suisan.52.1209
  28. Tripp, R. A., Maule, A. G., Schreck, C. B. and Kaattari, S. L : Cortisol-mediated suppression of salmonid lymphocyte response in vitro. Dev. Comp. Immunol., 11 : 565-576, 1987 https://doi.org/10.1016/0145-305X(87)90045-0
  29. Wedemeyer, G. A. and McLeay, D. J. :Methods for determining the tolerance of fishes to environmental stressors. In: Stress and Fish (ed. by Pickering, A. D.), pp. 247-275. Academic Press, London, 1981
  30. Wise, D. J. : Effects of stress on susceptibility of naive Channel Catfish in immersion challenge with Edwardsiella ictaluri. J. Aquat. Animal Health, 5 : 92-97, 1993 https://doi.org/10.1577/1548-8667(1993)005<0092:EOSOSO>2.3.CO;2
  31. Whyte, S. K., Chappell, L. H. and Secombes, C. J. : Cytotoxic reaction of rainbow tmut, Salmo gairdneri Richardson, macrophages for larvae of the eye flu ke, Diplostomum spathaceum. (Digenia). J. Fish Biol., 35 : 333-345, 1989 https://doi.org/10.1111/j.1095-8649.1989.tb02986.x
  32. 김인배 : 어류양식. pp. 323-329, 신흥출판사, 1993
  33. 김진우, 박수일, 전세규 : 양식넙치로 부터의 lysozyme 정제와 어류 병원성 세균에 대한 정균작용, 한국어병학회지, 5 : 87-92, 1992
  34. 장선일, 김강주 : 세포학 및 면역학 실험노트, pp. 179-182, 원광대학교 출판국, 1996
  35. 장영진, 박명룡, 강덕영, 이복규 : 양식넙치(Paralichthys olivaceus)의 생리에 미치는 연속적인 수온 급강하의 영향. 한국수산학회지. 32 : 601-606, 1999