The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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Chemopreventive Effects of the Extracts from Soybean Fermented with Basidiomycetes

들송이(Agrocybe cylindracea)와 말똥진흙버섯(Phellinus igniarius)을 배양한 대두추출물의 암예방 효과

  • Kim, So-Yeun (Dept. Food Science and Technology, Yeungnam University) ;
  • Son, Jun-Ho (Korea Advanced Food Research Institute) ;
  • Ha, Hyo-Cheol (Dept. Food Science and Technology, Yeungnam University) ;
  • Lee, Hang-Woo (T. M. R. center, Keimyung University) ;
  • Lee, Jae-Sung (Dept. Food Science and Technology, Yeungnam University)
  • 김소연 (영남대학교 식품가공학과) ;
  • 손준호 (한국식품연구소) ;
  • 하효철 (영남대학교 식품가공학과) ;
  • 이항우 (계명대학교 전통미생물 자원센터) ;
  • 이재성 (영남대학교 식품가공학과)
  • Published : 2002.10.30
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Abstract

The inductions of phase II enzymes, such as NAD(P)H : quinone reductase (QR), glutathione S-transferase (GST), glutathione (GSH) level and the inhibition of polyamine metabolism were tested for the chemopreventive potentials of the extracts from the soybean fermented with Agrocybe cylindracea (AC) or Phellinus igniarius (PI). The soybean fermented with AC or PI was potent inducer of QR activity in murine hepatoma Hepa1c1c7 cells. GST activities of the extracts from soybean fermented with AC or PI were higher than that of the extract from soybean not fermented with basidiomycetes. In addition, GSH levels of the extracts from soybean fermented with AC or PI were increased about 1.2 fold or 1.4 fold, respectively. In addition, proliferation of Acanthamoeba castellanii in a broth medium was inhibited by the extracts from soybean fermented with AC or PI at the both concentration of 20 and 40 mg/3 ml. These results suggest that soybean fermented with AC or PI may have chemopreventive potentials by inducing QR activity, increasing GSH and GST levels and inhibiting polyamine metabolism.

본 연구에서는 버들송이(Agrocybe cylindracea)와 말똥진흙버섯(Phellinus igniarius)을 배양한 대두 추출물의 암예방 효과를 검토하기 위하여 in vitro상에서 암발생 억제물질 연구에 널리 이용되고 있는 기작 즉, Quinone reductase(QR), glutathione S-transerase(GST)의 활성 유도, glutathione(GSH)의 생성 촉진 그리고, polyamine 합성대사의 억제능을 측정하였다. 그 결과, 버들송이와 말똥진흙버섯를 배양한 대두 추출물이 마우스 hepatoma Hepa1c1c7 cell 배양에서 QR과 GST 유도활성이 있음이 확인되었다. 또한 GSH 생성량은 버들송이를 배양한 대두 추출물에서 20%, 말똥진흙버섯를 배양한 대두 추출물에서 40%로 증가하였다. 한편, 버들송이와 말똥진흙버섯을 배양한 대두 추출물의 polyamine 합성대사의 억제효과를 검토하기 위해 Acanthamoeba castellanii의 증식을 측정하였다. 그 결과, 버들송이와 말똥진흙버섯을 배양한 대두 추출물 20mg/3ml와 40mg/3ml를 처리할 경우 A. castellanii의 증식을 억제하는 것으로 나타났다. 이러한 결과 담자균을 배양한 대두 추출물은 in vitro상에서 나타난 암과 관련된 각종 활성으로부터 암예방 효과가 있는 것으로 나타나 기능성 식품으로서의 가능성을 보여주었다.

Keywords

References

  1. Benson, A. M. and Barretto, P. B. 1985. Effects of disulfiram, diethydithiocarbamate, bisethylxanthogen, and benzyl isothiocyanate on glutathione transferase activities in mouse organs Cancer Res. 45: 4219-4223.
  2. Bora, P. S., Spilhurg, C. A. and Lange, L. G. 1989. Metabolism of ethanol and cracinogens by glutatbione transferases. Proc. Natl. Acad. Sci. 86: 4470-4473. https://doi.org/10.1073/pnas.86.12.4470
  3. Bull, A. W., Nigro, N. D., Golembieski, W. A., Crissman, J. D., Marnett, L. J. 1984. In vivo stimulation of DNA synthesis and induction of ornithine decarboxylase in rat colon by fatty acid hydroperoxides, autoxidation products of unsaturated fatty acids. Cancer Res. 44: 4924-4928.
  4. Byers, T. J., Akins, R. A., Maynard, B. J., Lefken, R. A., Martin, S. M. 1980. Rapid growth of Acanthamoeba in defined media: Induction of encystment by glucose-acetate starvation. J. Protozool.27: 216-219. https://doi.org/10.1111/j.1550-7408.1980.tb04684.x
  5. Choi, M. S., Lee, J. H., So, J. N. and Kim, J. M. 1990. Effect of lentinan on the immune function. Kor. J. Immunol. 12: 235-249.
  6. Griffith, O.W. 1980. Determination of glutathione and glutathione disulfide using glutathione reductase and 2-vinylpyridinc. Anal. Biochem. 106: 207-212. https://doi.org/10.1016/0003-2697(80)90139-6
  7. Habig, W. H., Pabst, M. H. and Jacoby, W. B. 1974. Glutathione S-transferase : the first enzymatic step mercapturic acid formation. J. Biol. Chem. 249: 7130-7139.
  8. Kim, B. G., McCann, P. P. and Byers, T. J. 1987. Inhibition of multiplication in Acanthamoeba castellanii by specific inhibition of ornithine decarboxylase. J. Protozool. 34: 264-266. https://doi.org/10.1111/j.1550-7408.1987.tb03172.x
  9. Kim, H. S., Kacew, S. and Lee, B. M. 1999a. ln vitro chemopreventive effects of plant polysaccharides (Aloe barbadensis Miller, Lentinus edodes, Ganoderma lucidum and Corious versicolor). Carcinogenesis 20: 1637-1640. https://doi.org/10.1093/carcin/20.8.1637
  10. Kim, J. W., Choi, H. K., Shan, Y. H., Lim, J. K., Lee, H. W., Nam, K. S. 1999b. Chemopreventive potential of Lonicerae flos aqua-acupuncture solution. Kor. J. Pharmacogn. 30: 261-268.
  11. Kim, S. Y., Shon, Y. H., Lee, J. S., Kim, C. H. and Nam. K. S. 2000. Antimutagenie activity of soybeans fermented with basidiomycetes in Ames/Salmonella test. Biotech. Letters 22: 1197-1202. https://doi.org/10.1023/A:1005697515592
  12. Kingsnorth, A. N., King, W. W. K., Diekema, K. A., McCann, P. P., Ross, J. S., Malt, R. A. 1983. Inhibition of ornithine decarboxylase with 2-difiuoromethylomithine : Reduced incidence of dimethylhydrazine-induced colon tumors in mice Cancer Res. 43: 2545-2549.
  13. Lee, J. S., Park, S. and Park, G. S. 1989. Optimization of media composition and cultivation for the mycelial growth of Agrocybe cylindracea. Kor. J. Food Sci. Technol. 21: 399-403.
  14. Mitchell, J. R., Hinson, J. A. and Nelson, S. D. 1976. Glutathione and drug induced tissue lesions: metabolism and function. pp. 357-367. In : Arias, I. M. and Jakoby, W. B. Eds. Glutathione. Raven Press, New York.
  15. Morigiwa, A., Kitabatake, K., Fujimoto, F., Ikekawa, N. 1986. Angiotensin converting enzyme inhibitory triterpenes from Ganoderma lucidium. Chem. Pharm. Bull. 34: 3025-3028. https://doi.org/10.1248/cpb.34.3025
  16. Pegg, A. E. 1988. Polyamine metabolism and its importance in neoplastic growth and as a target for chemotherapy. Cancer Res. 48: 759-774.
  17. Prochaska, H. J., Talalay, P. and Sies, H. 1987. Direct protective effect of NAD(P)H : quinone reductase against menadioneinduced chemiluminescence of postmitochondrial fractions of mouse liver. J. Biol. Chem. 262: 1931-1934.
  18. Prochaska, H. J. and Santamaria, A. B. 1988. Direct measurement of NAD(P)H : Quinone reductase from cells cultured in microtiter wells: A screening assay for anticarcinogenic enzyme inducers. Anal. Biochem. 169: 328-336. https://doi.org/10.1016/0003-2697(88)90292-8
  19. Rozhin, J., Wilson, P. S., Bull, A. W. and Nigro, N. D. 1984. Ornithine decarboxylase activity in the rat and human colon Cancer Res. 44: 3226-3230.
  20. Shanna, S., Stutzman, J. D., Kelloff, G. J. and Steele, V. E. 1994. Screening of potential chemopreventive agents using biochemical markers of carcinogenesis. Cancer Res. 54: 5848-5855.
  21. Shimizu, A. Yano, T., Saito, Y. and Inada, Y. 1985. Isolation of an inhibitors platelet aggregation from a fungus, Ganoderma lucidum. Chem, Pharm. Bull. 33: 3012-3015. https://doi.org/10.1248/cpb.33.3012
  22. Shon, Y. H. and Nam, K. S. 2001. Antimutagenicity and induction of anticarcinogenic phase II enzymes by basidiomycetes J. Ethnopharmacol. 77: 103-109. https://doi.org/10.1016/S0378-8741(01)00276-8
  23. Yamamura. Y. and Cochran, K. W. 1976. A selective inhibitor of myxoviruses from shiitake (Lentinus edodes). Mushroom Sci. 9(part 1): 495.
  24. Yiu, T. C. and Ushio, S. 1989. Screening of antiallergic effect in traditional medicinal drugs and active constituents of Aurantii fructus Immaturus. Shoyakugaku Zasshi 43: 314.
  25. Yoon, J. O., Min, T. J. and Yoon, H. S. 1995. An antibacterial lectin from Lamptermoyces japonicus. Kor. J. Micol. 23: 46-52.
  26. Wang, H. X., Liu, W. K., Ng, T. B. Ooi, V. E. C. and Chang, S. T. 1995. Immunomodulatory and antitumor activities of a polysaccharide-peptide complex from a mycelial culture of Tricholoma sp., a local edible mushroom. Life Sci. 57: 269-281. https://doi.org/10.1016/0024-3205(95)00270-G
  27. Wattenberg, L. W. 1985. Chemoprevention of cancer. Cancer Res 45: 1-8. https://doi.org/10.1016/S0065-230X(08)60265-1
  28. Wattenberg, L. W. and Bueding, E. 1986. Inhibitory effects of 5-(2-pyrazinyl)-4-methyl-1,2-dithiol-3-thione (Oltipraz) on carcinogenesis induced by benzo(a)pyrene, diethylnitrosamine and uracil mustard. Carcinogenesis 7: 1379-1381. https://doi.org/10.1093/carcin/7.8.1379

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