The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
권호 표지
DOI QR코드

DOI QR Code

Cultural Characteristics of Mycelial Growth by Cordyceps militaris

번데기동충하초(Cordyceps militaris)의 균사 생장

  • Sung, Jae-Mo (Department of Environmental Biology, Kangwon National University) ;
  • Choi, Young-Sang (Department of Environmental Biology, Kangwon National University) ;
  • Shrestha, Bhushan (Department of Environmental Biology, Kangwon National University) ;
  • Park, Young-Joon (Department of Environmental Biology, Kangwon National University)
  • Published : 2002.04.30
Download PDF
⟨ Previous Next ⟩

Abstract

Cultural characteristics of Cordyceps militaris preserved in EFCC, Kangwon National University were investigated for the mass production. The higher mycelial density of C. militaris was observed in Sabouraud's yeast and Yeast Malt agars, but the higher mycelial growth in Mushroom Minimal agar than other agars. The mycelium of C. militaris was observed to grow well at $25^{\circ}C$ and pH 6.0 respectively. The dextrose was found the best suitable energy source among the carbohydrates used for its mycelial growth, while the fructose or lactose observed to be well for mycelial growth. Hemoglobin was observed to be the best among the protein sources used for mycelial growth, while tryptone found to be the best in the spore formation. Similarly, the mycelial growth was best in mineral salts of $KH_2PO_4$ or $K_2HPO_4$ and the optimum C/N ratio was 100 : 1.

강원대학교 EFCC에서 보관중인 번데기동충하초의 배양적 특성과 인공재배의 조건을 조사하였다. 균사생육에 적합한 배지로는 SDAY 배지와 YMA 배지, SDAY 배지, HMD 배지, PDA 배지로 조사되었으며 균사생육이 빠른 배지로는 MMM 배지로 조사되었다. 균사생장에 적합한 온도로는 $25^{\circ}C$와 pH는 $6.0{\sim}8.0$으로 조사되었다. 균사배양에 적합한 탄소원으로는 dextrose로 조사되었고 fructose와 lactose에서 균사생장이 빠른 편이였다. 균사생장이 우수한 질소원으로는 hemoglobin으로 나타났으며 tryptone에서 포자형성이 우수하였다. 무기 염류에서는 $KH_2PO_4$와 $K_2HPO_4$에서 균사생장이 우수하였으며 C/N비에서는 100 : 1의 비율에서 균사생장이 양호하였다.

Keywords

References

  1. 金相姫. 1998. 韓國産 번데기冬蟲夏草의 分子生物學的 分類. 江原大學校 碩師學位 論文.
  2. 성재모. 1996. 한국의 동충하초. 교학사. 62-90 pp.
  3. 李賢卿. 1996. 韓國産 冬蟲夏草의 分布와 分類 및 培養的特性에 관한 研究. 江原大學校 碩師學位 論文.
  4. Basith, M. and Madelin, M. F. 1968. Studies on the production of perithecial stromata by Cordyceps militaris in artificial culture. Can. J. Bot. 46: 473-480. https://doi.org/10.1139/b68-071
  5. Brady, B. L. K. 1979. Cordyceps militaris. In: CMI Descriptions of pathogenic fungi and bacteria No. 605.
  6. Choi, I. Y, Choi, J. S., Lee, W. H., Yu, Y. J., Joung, G. T., .Ju, I. O. and Choi, Y. K. 1999. The condition of production of artificial fruiting body of Cordyceps militaris. Kor. J Mycol. 27(4): 243-248.
  7. Cunningham, K. G., Hutchinson, S. A., Manson, W. and Spring, F. S. 1951. Cordycepin, a metabolic product form cultures of Cordyceps militaris (Linn.)Link. Pt. 1. Isolation and characteristcs. J. Chem. Soc. 2299-2300. https://doi.org/10.1039/jr9510002299
  8. Furuya, T., Hirotani, M. and Matsuzawa. M. 1983. $N^6-(2-Hydroxyethyl)adenosine$, a biologically active compound form cultured mycelia of Cardyceps and Isaria species. Phytochemistry 22(11): 2509-2512. https://doi.org/10.1016/0031-9422(83)80150-2
  9. Glare, T. R. and Milner, R. J. 1991. Ecology of entomopathogenic fungi. In: Handbook of Applied Mywiogy. Vol. 2. Ed. D. K. Arora, L. Ajello, and K. G. Mukergi. Marcel Dekker, Inc.
  10. Humber, R. A. 2000. Fungal pathogens and parasites of insects. In: Applied Microbial Systematics. Ed. F. G. Priest and M. Goodfellow. Kluwer cademic Publishers.
  11. Hung, Y F., Thomason, M. J., Rhys-williams, W., Lioyd, A. W. and Hanlon, G. W. 1996. Chiral inversion of 2-phenylpropionic acid by Cordyceps militaris. J. Appl. Bacterial. 81: 242-250. https://doi.org/10.1111/j.1365-2672.1996.tb04324.x
  12. Iwashima, A., Kawasaki, Y., Nosaka. K. and Nishimura, H. 1992. Effect of thiamin on cordycepin sensityvity in Saccharomyces cerevisiae. FEBS Letters 311(1): 60-62. https://doi.org/10.1016/0014-5793(92)81367-U
  13. Kobayasi, Y. 1941. The genus Cordyceps and its allies. Set. Rept. Tokyo Buntika Daigaku, sect. B.5: 53-260.
  14. Kobayasi, Y. 1982. Keys to the taxa of the genera Cordyceps and Torrubiella. Trans. Mycol. Soc. Japan 23: 329-364.
  15. Mains, E. B. 1958. North American entomogenous species of Cordyceps. Mycologia 50: 169-222. https://doi.org/10.2307/3756193
  16. Pellit. R. H. 1895. Studies in artificial cultures of entomogenous fungi. Cornell Univ. Agric. Exp. Sta. Bull. 97: 417-465.
  17. Samson, R. A. 1974. Paecilomyces and some allied Hyphomycetes. Studies in Mycology 6: 31-36.
  18. Samson, R. A. 1995. Constraints associated with taxonomy of biocontrol fungi. Can. J. Bot. 73(S1); S83-S88. https://doi.org/10.1139/b95-229
  19. Seaver, F. J. 1911. The Hypocrcalcs of North America-IV. Mycologia 3: 207-230. https://doi.org/10.2307/3753505
  20. Sopp, J. O. O. 1911. Untersuchungen uber insektenvertilgende Pilze bei den letzten Kieferspinncrcpidemien in Norwegen. Skr. VidenskSelsk. Christiania. pp. 1-56.
  21. Sung, J. M., Choi, Y., S., Lee, H. K., Kim, S. H., Kim, Y. O. and Sung, G. H. 1999. Production of fruiting body using cultures of entomopathogenic fungal species. Kor. J Mycol. 27(l): 15-19.
  22. Sung, J. M., Kim, C., H., Yang, K. J., Lee, H. K. and Kim, Y. S. 1993. Studies on distribution and utilization of Cordyceps militaris and C. nutans. Kor. J. Mycol. 21(2): 94-105.
  23. Sung, J. M., Lee. H. K., Choi, Y. S., Kim, Y. O., Kim. S. H. and Sung, G. H. 1997. Distribution and taxonomy of entomopathogenic fungal species from Korea. Kor. J. Mycol. 25(4): 231-252.
  24. Sung, J. M., Lee. H. K., Yoo, Y. J., Choi, Y. S., Kim, S. H., Kim, Y. O. and Sung. G. H. 1998. Classification of Cordyceps species based on protein banding pattern. Kor. J. Myeol. 26(1): 1-7.
  25. Wang, Z. N. 1989. Cultural characteristics of imported Cordyceps fungi and their pathogenicity to some insects. Rep. Taiwan sugar Res. Ins. 126: 13-25.
  26. Ying, J., Mao X., Ma. Q., Zong, Y. and Wen, H. 1987. Icons of medical fungi from China. Science press, Beijing, China.
  27. Zeng, M. and Kinjo, N. 1998. Notes on the alpine Cordyceps of China and nearby nations. Mycotaxon 46: 215-229.

Cited by

  1. Antitumor Activity of Water Extracts From Cordyceps Militaris in NCI-H460 Cell Xenografted Nude Mice vol.2, pp.4, 2009, https://doi.org/10.1016/S2005-2901(09)60071-6
  2. Cultural Characteristics ofOphiocordyceps heteropodaCollected from Korea vol.39, pp.1, 2011, https://doi.org/10.4489/MYCO.2011.39.1.001
  3. Observations on Some of the Mycelial Growth and Pigmentation Characteristics ofCordyceps militarisIsolates vol.34, pp.2, 2006, https://doi.org/10.4489/MYCO.2006.34.2.083
  4. Cultural Characteristics ofShimizuomyces paradoxusCollected from Korea vol.38, pp.3, 2010, https://doi.org/10.4489/MYCO.2010.38.3.189
  5. Characteristics ofMetacordyceps yongmunensis, a New Species from Korea vol.38, pp.3, 2010, https://doi.org/10.4489/MYCO.2010.38.3.171