Herbal Formula Science (대한한의학방제학회지)

The Korean Medicine Society for the Herbal Formula Study (대한한의학방제학회)
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Apoptosis of 4-Acetyl-12, 13-epoxyl-9-trichothecene-3, 15-diol isolated from the fruiting bodies of Isaria japonica Yasuda via Caspase-3 in Bladder cancer Cell line (NBT-II)

Caspase-3을 경유한 동충하초 자실체 유래 4-Acetyl-12, 13-epoxyl-9-trichothecene-3, 15-diol의 방광암 세포주(NBT-II) Apoptosis

  • Kim Hyeong-Jin (Department of Microbiology and Immunology, Wonkwang University, School of Medicine) ;
  • Jang S.I. (Department of Microbiology and Immunology, Wonkwang University, School of Medicine) ;
  • Oh K,S. (Medicinal Resources Research Center of Wonkwang University) ;
  • Hong K.H. (Medicinal Resources Research Center of Wonkwang University) ;
  • Kim Y.C. (Medicinal Resources Research Center of Wonkwang University) ;
  • Pae H.O. (Medicinal Resources Research Center of Wonkwang University) ;
  • Yun Y.G. (Medicinal Resources Research Center of Wonkwang University) ;
  • Chung H.T. (Medicinal Resources Research Center of Wonkwang University) ;
  • Kwon T.O. (Medicinal Resources Research Center of Wonkwang University)
  • 김형진 (원광대학교 의과대학 미생물 및 면역학교실) ;
  • 장선일 (원광대학교 의과대학 미생물 및 면역학교실) ;
  • 오기수 (원광대학교 의약자원연구센터) ;
  • 홍경환 (원광대학교 의약자원연구센터) ;
  • 김윤철 (원광대학교 의약자원연구센터) ;
  • 배현옥 (원광대학교 의약자원연구센터) ;
  • 윤용갑 (원광대학교 의약자원연구센터) ;
  • 정헌택 (원광대학교 의약자원연구센터) ;
  • 권태오 (원광대학교 의약자원연구센터)
  • Published : 2002.12.30
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Abstract

The fruiting bodies of Isaria japonica have been traditionally used in Korea to treat cancer. An apoptosis-inducing compound, 4-Acetyl-12, 13-epoxyl -9-trichothecene-3, I5-diol (AETD), was isolated from the methanol extract of fruiting bodies of Isaria japonica Yasuda by bioassay -guided fractionation. The apoptosis of murine bladder cancer cell line (NBT-Ⅱ) by the compound was accessed by propidium iodide staining flow cytometric analysis, and apoptosis-inducing activity at $IC_{50}$ concentration (5 nmol／L) was further confirmed by a nuclear morphological change, a ladder pattern of DNA fragmentation, and an activation of caspase-3. These results indicate that AETD induces apoptosis of NBT-Ⅱ cells via expression of caspase-3.

Keywords

References

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