Applied Biological Chemistry

  • 제45권4호
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  • Pages.203-206
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  • 2002
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  • 2468-0834(pISSN)
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  • 2468-0842(eISSN)
한국응용생명화학회 (The Korean Society for Applied Biological Chemistry)
권호 표지

들깨 ${\gamma}-tocopherol$ methyltransferase cDNA 유전자의 분리 및 특성

Molecular Cloning and Characterization of ${\gamma}-tocopherol$ Methyltransferase cDNA from Perilla frutescens

  • 황선갑 (농촌진흥청 농업생명공학연구원 신기능소재개발과) ;
  • 김동헌 (농촌진흥청 농업생명공학연구원 신기능소재개발과) ;
  • 이재열 (경북대학교 자연과학대학 미생물학과) ;
  • 김용환 (농촌진흥청 농업생명공학연구원 신기능소재개발과) ;
  • 황영수 (농촌진흥청 농업생명공학연구원 신기능소재개발과) ;
  • 김경환 (농촌진흥청 농업생명공학연구원 신기능소재개발과)
  • Hwang, Seon-Kap (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration) ;
  • Kim, Dong-Hern (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration) ;
  • Lee, Jai-Youl (Department of Microbiology, Kyungpook National University) ;
  • Kim, Young-Hwan (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration) ;
  • Hwang, Young-Soo (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration) ;
  • Kim, Kyung-Hwan (Plant Metabolic Engineering Division, National Institute of Agricultural Biotechnnology, Rural Development Administration)
  • 발행 : 2002.11.30
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초록

${\gamma}-Tocopherol$ methyltransferase(TMT)는 토코페롤 생합성 대사의 마지막 단계인 감마 토코페롤을 알파 토코페롤로 변환하는데 관여하는 효소이다. 들깨의 미성숙 종자 cDNA유전자 은행에서 TMT로 추정되는 유전자를 분리하였으며 이 유전자는 1369개의 염기와 367개의 아미노산으로 구성되었으며 분자량은 약 42kDa의 추정된다. 이 cDNA는 Genbank와 상동성 분석결과 애기장대의 TMT유전자와 아미노산 수준에서 60% 정도의 상동성을 가지고 있으며 methyltransferase domain과 S-adenosyl methionine binding domain을 가지고 있으므로 TMT 유전자로 추정했다. 이 유전자의 특성을 알기 위하여 완전한 크기를 가지는 TMT유전자를 대장균에서 발현하고 invitro에서 효소의 활성을 측정하였다.

${\gamma}-Tocopherol$ methyltransferase (TMT) is an enzyme catalyzing ${\gamma}-tocopherol$ into ${\alpha}-tocopherol$ at the final step of ${\alpha}-tocopherol$ synthesis pathway. Putative TMT cDNA clone specific to Perilla frutescens immature seeds was isolated from cDNA library. The cDNA clone consisted of 1369 bp open reading frame encoding 369 amino acids with a relative Mw of 42 kDa. Results revealed the CDNA has 60% homology to Arabidopsis thaliana TMT, and possesses methyltransferase and S-adenosyl methionine-binding domains, suggesting that cDNA encodes a ${\gamma}-tocopherol$ methyltransferase To characterize the properties of the TMT gene, the cDNA sequences coding for mature TMT were expressed in E. coli and assayed to determine the enzyme activity in vitro.

키워드

참고문헌

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