한국약용작물학회지 (Korean Journal of Medicinal Crop Science)

  • 제10권2호
  • /
  • Pages.88-92
  • /
  • 2002
  • /
  • 1225-9306(pISSN)
  • /
  • 2288-0186(eISSN)
한국약용작물학회 (The Korean Society of Medicinal Crop Science)
권호 표지

대황의 형질전환된 뿌리로 부터 anthraquinone의 생산

Anthraquinone Production in Transformed Roots of Rheum undulatum L.

  • 황성진 (동신대학교 식품생물공학과) ;
  • 표병식 (동신대학교 식품생물공학과) ;
  • 채호준 (전남대학교 생물학과) ;
  • 황백 (전남대학교 생물학과)
  • 발행 : 2002.06.30
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

대황(Rheum undulatum L.)의 형질전환된 뿌리의 기내(in vitro)배양으로부터 quinone계 알칼로이드 물질인 anthraquinone의 생산성을 확인하고자 하였다. 형질전환된 뿌리로부터 anthraquinone의 생산에 있어서 배지, 초기 pH, 탄소원의 농도, 광 그리고 elicitors의 영향을 조사 하였다. 형질전환된 뿌리는 6% sucrose와 0.5 mg/l $GA_3$ 그리고 50 mg/l chitosan이 첨가된 WPM 배지에 치상한 후 16/8의 광조건$(16{\mu}mol\;m^2s^1)$하에서 배양시 0.18 mg(생중량)의 anthraquinone의 생산성을 보여주었다. 이와같은 함량은 재배근의 약 1.3배에 해당된다.

The production of anthraquinone has been dectected in transformed roots of Rheum undulatum L. The effects of medium, initial pH, concentration of sucrose, light irradiation and elicitors on anthraquinone production in transformed roots of Rheum undulatum L. were investigated. The maximum production of anthaquinone was achieved in WPM medium (pH 5.7) supplemented with 6% sucrose, 0.5 mg/l $GA_3$, and 50 mg/l chitosan at 16h light $(16{\mu}mol\;m^{-2}s^{-1})$ condition. Under the optimum conditions, the production of anthraquinone reached to 0.18 mg/g(F.W.) after 8 weeks. The content was estimated about 1.3 times of the level of native roots.

키워드

참고문헌

  1. Arro RRJ, Develi A, Meijers H, van der Westerlo E, Cores AK, Wullems GJ (1995) Effects of endogenous auxin on root morphology and secondary metabolism in Tagetes patula hairy root cultures. Physiol. Plant. 93 : 233-240 https://doi.org/10.1111/j.1399-3054.1995.tb02222.x
  2. Bakkali AT, Jaziri M, Ishimam K, Tanaka N, Shimomura K(1997) Tannin production in hairy root. cultures of Lawsonia inermis. J. Plant Physiology 151 : 505-508 https://doi.org/10.1016/S0176-1617(97)80019-3
  3. Chang SW, Kim IH, Han TJ (1999) Anthroquinone productivity by the cultures of advantitious roots and hairy roots from Curled DocK(Rumex crispus). Kor. J. Plant-Tiss. Cult. 26 : 7-14
  4. Flores HE (1987) Use of plant cell and organ culture in the production of biological chemicals. In Applications of Biotechnology to Agricultural Chemistiy. American Chemical Society Symposium Series, Vol. 334. H. Lebaron, R.O. Mumma, R.C. Honeycutt and J.H. Duesing ed. American Chemical Society, Washington DC, pp. 66-68
  5. Hamil JD, Parr R, Robins J, Rhodes MJ (1986) Secondary prcduct formation by cultures of Beta vulgaries and Nicotiana rustica transformed with Agrobacterium rhizogenes. Plant Cell Rep. 5: 111-114 https://doi.org/10.1007/BF00269247
  6. Hashimoto T, Yukimune Y, Yamada Y (1986) Tropane alkaloid production in Hyosyamus root cultures. J. Plant Physiol. 124:61-75 https://doi.org/10.1016/S0176-1617(86)80178-X
  7. Hwang SJ, Na MS, Pyo BS, Lee JB, Hwang B (2000) Production of tannin from hairy root cultures of Rheum undulatum L. Kor. J. Med. Crop. Sci. 8 : 250-258
  8. Kimura T, Paul PHB, Chung KS, Han BH (1996) In International collection of traditional and folk medicine World Sci. pp. 18-20
  9. Kittipongpatana N, Hock RS, Porter JR (1998) Production of solasodine by hairy root, callus, and cell suspension cultures of Solanum aviculare Forst. Plant Cell, Tissue, Organ Culture 52 : 133-143 https://doi.org/10.1023/A:1005974611043
  10. List PH, Horhammer L (1979) Hagers handbuch der pharmazeutishen praxis, vol. 6. Springer-verlag, Berlin Heidelberg, NY pp. 95-113
  11. Merkli A, Christen P, Kapetanidis JI (1997) Production of diosgenin by hairy root cultures of Trigonella Foenum graecum L. Plant Cell Rep. 16:632-636 https://doi.org/10.1007/BF01275505
  12. Ogasawara T, Chiba K, Tada M (1993) Production in high-yield naphtaquinone by a hairy root cultures of Sesamum indicum. Phytochemistry 33 : 1095-1098 https://doi.org/10.1016/0031-9422(93)85029-Q
  13. Ozeki Y, Komamine A (1986) Effects of growth regulators on the induction of anthocyanin synthesis in carrot cell suspension cultures, Plant Cell Physiol. 27 : 1361-1368 https://doi.org/10.1093/oxfordjournals.pcp.a077234
  14. Rhodes MJ, Parr AJ, Giulietti A, Arid EL (1994) Influence of exogenous hormones on the growth and secondary metabolite formation in transformed root cultures. Plant Cell, Tissue, Organ Culture 38 : 143-151 https://doi.org/10.1007/BF00033871
  15. Robins RJ, Bent ES, Rhodes MJC (1991) Studies on the biosynthesis of tropane alkaloids by Datura stramonium L. transformed root cultures. 3. The relationship between morphological integrity and alkaloid biosynthesis. Planta 185 : 385-390
  16. Shanks JV, Morgan J (1987) Plant hairy root culture. Current Opinion in Biotechnology 10 : 151-155 https://doi.org/10.1016/S0958-1669(99)80026-3
  17. Toivonen L (1993) Utilization of hairy root cultures for production of secondary metabolites. Biotechnol. Prog. 9 : 12-20 https://doi.org/10.1021/bp00019a002
  18. Toivonen L, Ojala M, Kaupinnen V (1991) Studies on the optimization of growth and indole alkaloid production by hairy root cultures of Catharanthus roseous. Biotechnol. & Bioeng. 37 : 673-680 https://doi.org/10.1002/bit.260370709
  19. Weathers PJ, Hemmavanh DD, Walcerz DB, Cheetham RD, Smith TC (1997) Interactive effects of nitrate and phophate salts, sucrose, and inoculum culture age on growth and sesquiterpene production in Artemisia annua hairy root cultures. In Vitro Cell. Dev. Biol. -Plant. 33 : 306-312 https://doi.org/10.1007/s11627-997-0056-0
  20. Yang DC, Kim YH, Kwon JN, Choi CH, Yang DC (1995b) Effects of light on activities of antioxidative enzymes in hairy root cultures of Phytolacca esculenta van Houtte. Kor. J Plant Tiss. Cult. 22 : 71-76
  21. Yang DC, Choi YH, Kim YH, Yun KY, Yang DC (1996) Growth and ginsenosides production of hairy root via light energy. Kor. J. Ginseng Sci. 20 : 318-324
  22. Yang DC, Kang HM, Lee KS, Kim YH, Kim DC (1997) Effects of pH, sucrose and vitamines on the growth and tropane alkaloid production of hairy roots of Datura stramonium var. Tatula. Kor. J. Plant Tiss. Cult. 24 : 143-148
  23. Zhu YP (1998) In Chinese materia medica. Harwood Acad. Press. pp. 231-238