Korean Journal of Medicinal Crop Science (한국약용작물학회지)

The Korean Society of Medicinal Crop Science (한국약용작물학회)
권호 표지

Genetic diversity and population structure of Atractylodes japonica $K_{OIDZ}.$ in Korea

한국내 삽주의 유전적 다양성과 집단구조

  • Published : 2002.03.31
Download PDF
⟨ Previous Next ⟩

Abstract

The study of genetic diversity was carried out in Atractylodes japonica $K_{OIDZ}$. Although this species has been regarded as medically important one, there is no report on population structure in Korea. Starch gel electrophoresis was used to investigate the allozyme variation and genetic structure of eight Korean populations of this species. Of the 15 genetic loci surveyed, nine (60.0%) was polymorphic in at least one population. Genetic diversity was high at the species level $(H_{es}=0.144)$, whereas, that of the population level was relatively low $(H_{ep}=0.128)$. Nearly 87% of the total genetic diversity in A. japonica was apportioned within populations. The sexual reproduction, high fecundity, and perennials are proposed as possible factors contributing to high genetic diversity. The indirect estimated of gene flow based on Gst was 1.69.

한국내 분포하는 다년생 초본인 삽주 집단의 유전적 다양도와 집단구조를 조사하기 위해 전분 전기영동으로 분석하였다. 15 대립유전자좌위당 9개 좌위에서 다형현상(60.0%)을 보였으며, 유전적 다양도는 종수준에서 0.144로 높은 반면 집단수준이 이보다 약간 낮았다. 삽주의 유전적 다양도는 대부분 집단내에 존재하였고 유사한 생활양식을 가진 다른 식물종에 비해 높았다. 그 이유로는 유성생식, 다년생, 다산 등에 기인한다. 집단간 분화는 약 13%였고 지리적 거리와 유전적 거리의 상관은 높았다(r=0.65). 그럼에도 불구하고 일부 격리된 집단은 유효집단크기를 가지지 못하여 이형접합체의 결여가 유의성을 보여 다양도가 높은 집단의 보존이 요망된다.

Keywords

References

  1. Edwards, A.L. and R.R. Sharitz. 2000. Population genetics of two rare perennials in isolated wetlands: Sagittaria isoetiformis and S. tens (Alismataceae). Am. J. Bot. 87 : 1147-1158 https://doi.org/10.2307/2656651
  2. Gottlieb, L.D. 1981. Electrophoretic evidence and plant populations. Prog. Phytochem. 7 : 1-46 https://doi.org/10.1016/S0031-9422(00)88197-2
  3. Hamhck, J.L. and M.J.W. Godt. 1989. Allozyme diversity in plant species, In: Plant Population Genetics, Breeding and Genetic Resources. Brown A.D.H., Clegg M.T., Kahler A.L. andWeir B.S. (eds), Sinauer, Sunderland, MA. pp. 43-63
  4. Hamrick J.L., M.J.W. Godt and S.L. Shennan-Broyles. 1992. Factors influencing levels of genetic diversity in woody plant species. New Forests 6 : 95-124 https://doi.org/10.1007/BF00120641
  5. Hartl, D.L. and A.G. dark. 1989. Principles of Population Genetics 2nd. Sinauer associates, Inc. Mass., pp. 682
  6. Huh, M.K. 1999. Genetic diversity and population structure of Korean alder (Alnus japonica : Betulaceae). Can. J. For. Res. 29:1311-1316 https://doi.org/10.1139/cjfr-29-9-1311
  7. Huh, M.K. 2001. Allozyme variation and population structure of Carex humilis var. nana (Cyperaceae) in Korea. Can. J. Bot. 79 : 457-463 https://doi.org/10.1139/cjb-79-4-457
  8. Lande, R. 1988. Genetis and demography in biological conservation. Science 241 : 1455-1460 https://doi.org/10.1126/science.3420403
  9. Li, C.C. and D.G. Horvitz. 1953. Some methods of estimating the inbreeding coefficient. Am. J. Hum. Genet. 5 : 107-117
  10. Loveless, M.D. and J.L. Hamiick. 1988. Genetic organization and evolutionary history in two North American species of Cirsium. Evolution 42 : 254-265 https://doi.org/10.2307/2409230
  11. Nei, M. 1972. Genetic distance between populations. Am. Nat 106 : 282-292
  12. Nei, M. 1973. Analysis of gene diversity in subdivided populations. Proc. Natl. Acad. Sci. USA. 70 : 3321-3323 https://doi.org/10.1073/pnas.70.12.3321
  13. Nei, M. 1977. F-statistics and analysis of gene diversity in subdivided populations. Ann. Hum. Genet. 41 : 225-233 https://doi.org/10.1111/j.1469-1809.1977.tb01918.x
  14. Nevo, E., A, Beiles and R. Ben-Shlomo. 1984. The evolutionary significance of genetic diversity : ecological, demographic and life history correlates, In : Evolutionaiy Dynamics of Genetic Diversity. G.S. Many (ed.), Sphnger Verlag, Berlin, pp. 13 21
  15. SAS Institute Inc. 1989. SAS/STAT User's Guide : Ver. 6. SAS Institute. Cary NC
  16. Smouse, P.E., J.C. Long and R.R. Sokal. 1986. Multiple regression and correlation extensions of the Mantel test of matrix correspondence. Syst. Zool. 35 : 627-632 https://doi.org/10.2307/2413122
  17. Soltis, D.E., C.H, Haufler, D.C. Danow and G.J. Gastony, 1983. Starch gel elect.rophoresis of ferns '. A compilation of grinding buffers, gel and electrode buffers, and staining schedules. Am. Pem J. 73:9-27
  18. Wahlund, S. 1928. Zusammersetung von Populationen und Korrelation-sercheiunungen von Standpunkt der Verebungslehre aus betrachtet. Hereditas ll: 65-106
  19. Workman, P.L. and J.D. Niswander. 1970. Population studies on southern Indian tribes. II. local genetic differentiation in the Papago. Am. J. Hum. Genet. 22 : 24-49
  20. Wright, S. 1951, The genetical structure of populations. Ann. Eug. 15 : 313-354
  21. Wright, S. 1965. The interpretation of population structure by F- statistics with special regard to systems of mating. Evolution 19 : 395-420 https://doi.org/10.2307/2406450