Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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The Nitrite Scavenging and Electron Donating Ability of Bamboo Smoke Distillates made by steel kiln and earth kiln

기계식 및 재래식 죽초액의 아질산염 소거 및 전자공여 작용

  • Lee, Fan-Zhu (Department of Food Science and Technology, Chonnam National University) ;
  • Choi, Sang-Ho (Biotechnology Research Institute, Chonnam National University) ;
  • Eun, Jong-Bang (Biotechnology Research Institute, Chonnam National University)
  • 이범수 (전남대학교 식품공학과) ;
  • 최상호 (전남대학교 생물공학연구소) ;
  • 은종방 (전남대학교 생물공학연구소)
  • Published : 2002.08.01
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Abstract

Fresh bamboo was charred in the mechanical steel kiln and traditional earth kiln, and then its smoke was condensed to produce bamboo smoke distillates (BSD). BSD made by mechanical steel kiln (MBSD) was purified by distillation. There is significant difference (p<0.05) in total phenol content among them, 663.40 ppm in MBSD, 571.62 ppm in PMBSD, and 485.16 ppm in TBSD. The nitrite scavenging activity and electron donation ability of BSDs were measured and the content of total phenol content was determined. The nitrite scavenging activity of BSD increased with increasing concentration of BSD at the same pH and decreased with increasing pH. There is significant difference (p<0.05) in nitrite scavenging activity among MBSD, purified MBSD (PMBSD), and BSD made by traditional earth kiln (TBSD) and the activity was the highest in MBSD and the lowest in TBSD. The electron donationg ability of BSDs increased with increasing the concentration of BSD and the reaction time. There is significant difference in the electron donation ability of BSDs at the same period and different concentration among the BSDs, the highest in MBSD and the lowest in TBSD.

신선한 왕대(Phyllostachys bambuoides bambuoides sieb. et zucc.)를 기계식 탄화로와 재래식 토요를 이용하여 탄화시켜 기계식 죽초액과 재래식 죽초액을 얻고 기계식 죽초액을 증류·정제하여 정제 죽초액을 얻은 다음 각각의 죽초액의 아질산염 소거와 전자공여 효과 및 이들의 페놀성 화합물의 함량을 조사하였다. 죽초액을 $1{\sim}6%$까지 6단계로 희석하여 각 농도에서의 아질산염 소거효과를 비교하였을 때 동일한 pH 하에서 농도가 증가할수록 활성이 증가하였다. 각각의 죽초액이 pH 1.2와 pH 3.0에서 뚜렷한 소거활성을 보였으나 pH가 증가할수록 점차적으로 낮아져 pH가 6.0일 때에는 미미한 상태였다. 각각의 죽초액이 일정한 농도와 pH에서 아질산염 소거효과는 유의적인 차이(p<0.05)를 나타내었으며 기계식 죽초액, 기계식 정제 죽초액, 재래식 죽초액의 순 이었다. 기계식 죽초액, 기계식 정제 죽초액, 재래식 죽초액의 총 페놀성 화합물의 함량은 유의적인 차이(p<0.05)를 보였고 각각 663.4, 571.62, 485.16 ppm이었으며 증류·정제과정에서 함량이 감소되었다. 각각의 죽초액을 $0.2{\sim}1.2%$까지 6단계로 희석하여 각 농도별 반응시간에 따르는 전자공여 작용 효과를 비교하였다. 농도가 증가할수록, 반응시간이 길어질수록 각각의 죽초액의 전자공여 작용 효과가 뚜렷하였고 일정시간 내에서 각 농도에서의 전자공여 작용 효과는 죽초액간에 유의적인 차이를 보였으며 기계식 죽초액이 가장 컸고 다음으로 기계식 정제 죽초액, 재래식 죽초액의 순 이었다. 이와 같은 결과는 재래식 죽초액이나 기계식 정제 죽초액보다 기계식 죽초액에 페놀성 화합물이 더 많이 함유되어서 나타난 것으로 생각된다.

Keywords

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