Korean Journal of Food Science and Technology (한국식품과학회지)

Korean Society of Food Science and Technology (한국식품과학회)
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Detection Characteristics of TL, ESR and DNA Comet for Irradiated Soybeans

열발광, 전자스핀공명 및 DNA Comet 분석에 의한 대두의 방사선 조사 여부 검지 특성

  • Lee, Eun-Young (Department of Food Science and Technology, Kyungpook National University) ;
  • Jeong, Jae-Young (Department of Food Science and Technology, Kyungpook National University) ;
  • Noh, Jung-Eun (Department of Food Science and Technology, Kyungpook National University) ;
  • Jo, Deok-Jo (Department of Food Science and Technology, Kyungpook National University) ;
  • Kwon, Joong-Ho (Department of Food Science and Technology, Kyungpook National University)
  • Published : 2002.02.01
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Abstract

The detection characteristics of gamma-irradiated $(0{\sim}4\;kGy)$ soybeans produced in Korea and China were investigated by thermoluminescene (TL), electron spin resonance (ESR), and DNA comet assay. The TL glow curves were shown at around $200^{\circ}C$ for irradiated soybeans, while that at $280^{\circ}C$ for the non-irradiated one. The normalization with a re-irradiation step at 1 kGy could verify the above detection results. The Korean soybean showed higher glow curves than Chinese did. The ESR spectroscopy for husks of irradiated soybeans revealed specific signals (g = 2.02374, 1.98715) derived from cellulose radical, which intensities were proportional to irradiation does, with the higher peaks in Chinese sample than Korean one. The DNA comet for the non-irradiated sample showed no or little tails, while those for irradiated samples above 0.5 kGy were remarkably changed in their length, size, and concentration, thus resulting in distinguishing non-irradiated from irradiated samples. As a result, TL, ESR, and DNA comet determinations were found suitable for the detection of irradiated soybean at 0.5 kGy or more, and negligible differences were observed between Korean and Chinese origins in their detection characteristics.

방사선 조사된 대두의 검지방법을 연구하기 위하여 국산 및 중국산 대두에 대하여 $0.5{\sim}4.0\;kGy$의 감마선을 조사하고 열발광(TL), 전자스핀공명(ESR) 및 DNA comet 특성을 비교 검토하였다. TL 측정에서 비조사구는 $280^{\circ}C$ 부근에서 매우 낮은 glow curve를 나타내었고, 감마선 조사구는 $200^{\circ}C$ 부근에서 조사선량에 의존적인 glow curve를 나타내었다. 재조사(re-irradiation) 방법에 의한 TL ratio$(TL_1/TL_2)$의 비교는 TL 측정 결과의 정확도를 높여 주었다. 원산지별 TL 특성 비교에서 국산을 중국산에 비해 높은 TL intensity를 나타내었다(p<0.01). 대두 껍질을 사용한 ESR 측정에서는 조사시료에서 cellulose radical 유래의 특이한 signal(g = 2.02374, 1.98715)을 보여주었고 중국산 시료의 ESR signal이 더 높게 나타났다. 비조사 대두의 DNA comet은 tail이 없거나 아주 짧은 tail을 가진 전형적인 intact cell을 나타내었다. 그러나 0.5 kGy 이상의 감마선 조사 시료에서는 조사선량에 의존적으로 comet의 tail length 증가와 더불어 comet의 크기 및 농도의 변화가 일어났다. 이상의 결과에서 TL, ESR 및 DNA comet의 분석은 대두의 방사선 조사 여부 확인을 가능하게 하였으며, 원산지별 검지특성의 차이는 거의 없었다.

Keywords

References

  1. WHO Wholesomeness of Irradiated Pood (Report of a joint FAO/IAEA/ WHO expert committee), Technical Report Seiies-659, 7-34 (1981)
  2. Codex Alimentanus Commission, Codex General Standard for Irradiated Foods and Recommended Intemational Code of Prac-tice for the Operation of Radiation Facilides Used for the Treat-ment of Poods. CAC/ VOL, XV, FAO, Rome (1984)
  3. IAEA homepage. www.iaea.org/icgfi (2001)
  4. Kwon, J.H. Import control of irradiated food. Food Industry 159(1): 61-87 (2001)
  5. Korea Agricultural Trade hiformadon homepage. www.kati.co.kr (2001)
  6. Kwon, Y.J., Huh, E.Y., Kwon, J.H. and Byun, M.W. Quarantine status of agncultural products for export and applicadon prospects ofirradiation technology. Food Sci. Ind. 32(2): 80-90 (1999)
  7. Kwon, J.H., Chung, H.W. and Kwon, Y.J. Infrastmcture of quar-antine procedures for promoting the trade of irradiated foods. Paper presented at Symposium of The Korean Society of Posthar-vest Science and Technology of Agricultural Products on Irradia-tion Technology for the Safety of Food and Public HealthIndustries and Quality Assurance. Daejon, 13 October, pp. 209-254 (2000)
  8. IAEA Analytical detection methods for irradiated foods. A review of current literature. IAEA-TECD0C-587, pp. 1-172 (1991)
  9. Delince, H. Detection of food treated with ionizing radiation. Trends in Food Sci. Tech. 9: 73-82 (1998) https://doi.org/10.1016/S0924-2244(98)00002-8
  10. Hwang, K.T., Park, J.Y. and Kwon, Y.J. Hydrocarbons detected in irradiated soybeans. Korean J. Food Sci. Technol. 30(3): 517-522 (1998)
  11. Lee, E.Y, Kim, M.O., Lee, H.J., Kim, K.S. and Kwon, J.H. Detection charactenstics of hydrocarbons from irradiated legumes of Korean and Chinese origins. J. Korean Soc. Food Sci. Nutr. 30(5): 770-776 (2001)
  12. Oh, K.N., Kim, K.E. and Yang, J.S. Detection of irradiated beans using the DNA comet assay. J. Korean Soc. Food Sci. Nutr. 29: 843-848 (2000)
  13. European Committee for Standard. Detection of irradiated food from which silicate minerals can be isolated. Method by ther-moluminescence. English version of DIN EN 1788 (1997)
  14. Chung, H.W. Characterization of irradiated foods by thermolumi-nescence and electron spin resonance measurements for their identification. Ph.D. thesis, Kyungpook National Univ., Daegu, Korea (2000)
  15. Schreiber, G.A., Hoffmann, A., Helle, N. and B$\ddot o$gl, K.W. An interlaboratory trial on the identification of irradiated spices, herbs, and spice-herb mixtures by thermoluminescence analysis. JAOAC Intl. 78: 88-93 (1995)
  16. Desrosiers, M.F. and Mclaughlin, W.L. Examination of gainma-irradiated fruits and vegetables by electron spin resonance spec-troscopy. Radiat. Phys. Chem. 34: 895-898 (1989)
  17. Stewart, E.M., Stevenson, M.H. and Gray, R. Use of ESR spec-troscopy for the detection of irradiated Crustacea. J. Sci. Food Agric. 65: 191-197 (1994) https://doi.org/10.1002/jsfa.2740650211
  18. Mckelvey-Martin, V.J., Green, M.H.L., Schmezer, P., Pool-Zobel, B.L., De M$\'{e}$, M. and Collins, A. The single cell electrophoresis assay (comet assay): A European review. Mutat. Res. 288: 47-63 (1993) https://doi.org/10.1016/0027-5107(93)90207-V
  19. Cerda, H., Delincee, H., Haine, H. and RupP, H. The DNA 'Comet assay' as a rapid screening technique to control irradiated food. Mutat. Res. 375: 167-181 (1997) https://doi.org/10.1016/S0027-5107(97)00012-2
  20. Origin. Origin tutorial manual, version 6.0, Microcal Software, Inc., pp. 20-45, Northampton, MA (1999)
  21. SAS, SAS users guide. SAS Institute Inc., Cary, NC, USA (1986)
  22. Kwon, J.H., Jeong, J., Chung, H.W. and Byun, M.W. Thermolu-minescence characteristics of minerals from irradiated potatoes of different ongind of production. Paper presented at llth Intema-tional Meeting on Radiation Processing. Avignon, France, 25-30 March (2001)
  23. Schreiber, G.A., Ziegelmann, B., Quitzsch, G., Helle, N. and B$\ddot o$gl, K.W. Luminescence techniques to identify the treatment of foods by ionizing irradiation. Food Stmcture 12: 385-396 (1993)
  24. Autio, T. and Pmnioja, S. Identification of irradiated foods the thermoluminescence of mineral contamination. Z. Sebensm. Unters. Forsch. 191: 177-180 (1990) https://doi.org/10.1007/BF01197616
  25. Khan, J.M. and Delinc$'{e}$e, H. Detection of radiation treatment of spices and herbs of asiac origin using thermoluminescence of mineral contaminants. Appl. Radiat. Isot. 46(10): 1071-1075 (1995) https://doi.org/10.1016/0969-8043(95)00193-H
  26. Goodman, B.A., McPhail, D.B. and Duthie, D.M.L. Electron spin resonance spectroscopy of some in-adiated food stuffs. J. Sci. Food Agric.47: 101-111 (1989) https://doi.org/10.1002/jsfa.2740470112
  27. Kwon, J.H., Chung, H.W. and Byun, M.W. ESR spectroscopy for detecting gamma-irradiated dried vegetables and estimating absorbed doses. Radiat. Phys. Chem. 57: 319-324 (2000) https://doi.org/10.1016/S0969-806X(99)00398-9
  28. Edgar, F.O. de Jesus, Rossi, A.M. and Lopes, R.T. An ESR study on identification of gamma-irradiated kiwi, Papaya and tomato using fruit pulp. Intl. J. Food Sci. Technol. 34: 173-178 (1999) https://doi.org/10.1046/j.1365-2621.1999.00250.x
  29. Jacques, J.R. and Lean-Pierre, L.A. Electron spin resonance iden-tification of irradiated fruits. Radiat. Phys. Chem. 34(6): 891-894 (1989)
  30. Lee, E., Jung, J., Chung, H.W. and Kwon, J.H. Effect of water activity on free radical concentration in detection of irradiated food by ESR spectroscopy. Paper presented at the 64th annual meeting of the Korean Society of Food Science and Technology, Daegu, May 22 (2000)
  31. Koppen, G. and Cerda, H. IdentiScation of low-dose irradiated seeds using the neutral comet assay. Food Sci. Technol. 30: 452-457 (1997)