Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
권호 표지

Protective Effects of Houttuynia cordata Thunb on Carbon Tetrachloride-induced Hepatotoxicity in Rats

어성초 분획물이 사염화탄소로 유발된 흰쥐의 간손상에 대한 보호효과

  • Published : 2002.12.30
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Abstract

This study was performed to investigate the protective effect of Houttuynia cordata Thunb on hepatotoxicity in carbon tetrachloride$(CCI_4)$ intoxicated rats. The examined effects hexane, chloroform, butanol and water fractions prepared from the Houttuynia cordata Thunb methanol exlract and rats were administrated with those orally once a day for successive 6 days, fellowed by treaoent with $CCl_4$ on the sixth day. After 6 days, the activities of aminotransferase, alkalinephosphatase, ${\gamma}-glutamyl$ transpeptidase, lactate dehydrogenase and contents of triglyceride, hepatic lipid peroxide in butanol fraction pretreated rats were significantly decreased compared to the only $CCl_4$ treated rats, also depletion glutathione content induced by treatment with $CCl_4$ was prevented by butanol fraction pretreated rats. In addition, activities of hepatic superoxide dismutase, catalase, glutathione peroxidase in butanol fiaction pretreated rats were significantly decreased compared to the only $CCl_4$ treated rats, but the activity of hepatic glutathione-S-transferase was not significantly effect. These results suggest that butanol fiuction of Houttuynia cordata Thunb methanol extract have potent hepatoprotective effect against carbon tetrachloride intoxicated rats.

Keywords

References

  1. Neuzil, J., Gebick, J. and Stocker, R. (1993) Radical induced chain oxidation of proteins and its inhibition by chain breaking antioxidants. Biochem. J. 293: 601-606 https://doi.org/10.1042/bj2930601
  2. Steinberg, D., Pathasarathly, S., Carew, T. E., Khoo, J. C. and Witztum, J. L. (1989) Beyond cholesterol. Modifications of low density lipoproteins that increase its atherogenicity. N. Engl. J. Med. 320: 915-923 https://doi.org/10.1056/NEJM198904063201407
  3. Gary, W. Pace and Cynthia, D. Leaf (1995) The role of oxidative stress in HIV disease, free radical. Biology and Medicine. 19: 523-528
  4. Gilman, A. G. (1985) Carbon tetrachloride. The Pharma cological basis of therapeutics 7: 1635-1636
  5. Bruckner, J. V., Mackenzie, W. F. and Muralidhara, S. (1986) Oral toxicity of carbon tetrachloride : Acute, subacute and subchronic studies in rats. Fund. Appl. Toxicology 6: 16-34 https://doi.org/10.1093/toxsci/6.1.16
  6. Butler, T. C. (1990) Reduction of carbon tetrachlohde in vivo and reduction of carbon tetrachloride and chloroform in vitro by tissues and constituents. J. Pharmacol. Exp. Ther. 134: 311-319
  7. Caragy, A. B. (1992) Cancer preventive foods and ingredients. Food Technology 46: 65-68
  8. Liang jun, Y., Marie, T. and Lester, P. (1995) Gingo bibba extract(EGb 761) Protects human low density lipoproteins against oxidative modification mediated by copper. Biochem. and Biophy. Res. Com. 212: 360-366 https://doi.org/10.1006/bbrc.1995.1978
  9. Kim, H. K., Kim, Y. E., Do, J. R., Lee, Y. C. and Lee, B. Y. (1995) Antioxidative activity and physiological activity of some korean medicinal plants. Korean J. Food Sci. Technol. 27: 80-85
  10. Middleton, E. (1996) Biological properties of plant flavonoids An Overview. Int. J. Pharmacognosy 34: 344-348 https://doi.org/10.1076/phbi.34.5.344.13245
  11. B. K. Hwan (2000) The medical plants of korea p.160 Kyohak pub. co
  12. Shanghai Science and Technological publisher(1985) The Dictionary of Chinese Drugs Vol. 1, Shougakukan Tokyo. p.507
  13. Lee, Y. J., Shin, D. H., Jang, Y. S. and Shin, J. I. (1993): Antioxidative effects of fractions from sequential ethanol extracts of Houttuynia cordata, Portulacaceae and sesame cake. J. Korean Soc. Food Nutr. 25: 683-686
  14. Hayashi, K., Kamiya, M. and Hayashi, T. (1995) Virucidal effects of the steam distillate from Houttuynia cordata and its components on HSV-l, influenza virus, and HIV. Ptanta Med. 61: 237-241 https://doi.org/10.1055/s-2006-958063
  15. Kim, S. K., Ryu S.Y., No, J., Choi, S. U. and Kim, Y. S(2001) Cytotoxic alkaloids from Houttuynia cordata. Arch. Pharm. Res. 24: 518-521 https://doi.org/10.1007/BF02975156
  16. Chang, V. S., Chiang, L. C., Chen, C. C., Liu, L. T., Wang, K. C. and Lin, C. C. (2001) Antileukemic activity of Biden-spilosa L. var. minor(B1ume) sherff and Houttuynia cordata Thunb. Am. J. Chin. Med. 29: 303-312 https://doi.org/10.1142/S0192415X01000320
  17. Chung, C. K., Ham, S. S., Lee, S. Y., Oh, D. H., Choi, S. Y., Kang I. J. and Nam, S. M. (1999) Effects of Houttyunia cordata ethanol extracts on serum lipids and antioxidant enzymes in mts fed high fat diet. J. Korean Soc. Food Sci. Nutr. 28: 205-211
  18. Lee, J. H., Jeong, S. I., You, I. S., Kim, S. K., Lee, K. N., Han, D. S. and Baek, S. H. (2001) The inhibitory effcts of the methanol extract of Houttuynia cordata Thunb against cadmium induced cytotoxicity(V). Kor. J. Pharmacogn. 32: 61-67
  19. Pak, J. C., Hur, J. M., Park, J. G., Park, S. J., Lee, J. H., Sung, N. J., Choi, M. R., Song, S. H., Kim, M. S. and Choi, J. W. (2000) The effects of Houttuynia cordata on the hepatic bromobenzene metabolizing enzyme system in rats and isolation of phenolic compounds. Kor. J. Pharmacogn. 31: 228-234
  20. Kim, K. Y., Chung, D. O. and Chung, H. J. (1997) Chemical composition and antimicrobial activities of Houttuynia cordata Thunb. Korean J. Food Sci. Technol. 29: 400-406
  21. Tutupalli, L. V. and Chaubal, M. G. (1975) Sauruaceae. V. composition of essential oil from foliage of Houttuynia cordata and chemosystematics of Saururaceae. Lloydia 38: 92-96
  22. Probstle, A., Neszmely, A., Jerkovich, G., Wagner, H. and Bauer, R. (1994) Novel pyndine and 1,4-dihydropyridine alkaloids from Houttuynia cordata. Natural Products Letters 4: 235-240 https://doi.org/10.1080/10575639408043911
  23. Tagagi, S., Yamaki, M., Masuda, K. and Kuboda, M.(1978) On the constituents of the terrestrial part Houttuynia cordata. Shoyakugaku Zasshi 32: 123-125
  24. Probstle, A., Lotter, H., Wagner-Redecker, W., Mathiesen, U. and Bauer, R. (1993) Identification of lipophilic constituents with antiinflammatory activity from Houttuynia cordata. planta Medica 59: 663-664
  25. Rao, V. C. and Nehendale, H. M. (1991) Colchicine antimiosis abolishes CC14 autoprotection. Toxicol. Pathol. 19: 597-606 https://doi.org/10.1177/019262339101900405
  26. Reitman, S. and Frankel, S. (1957) A colorimetric method for the determination of serum glutamic oxaloacetic and glutamic pyruvic transaminase. Am. J. Clin. Pathol. 28: 58-61
  27. Kawano, S., Nakagawa, H. and Toga, H. (1982) Investigation on physiological values of blood in industrial workers, Report 3. Serum colloid reaction and enzyme activity values. Sangyo Igaku 24: 275-283 https://doi.org/10.1539/joh1959.24.275
  28. Szaz, G. (1969) A kinetic photometric method for serum $\gamma$-glutamyltrans peptidase. Chin. Chem. 16: 124-136King, J. (1972) Effect of hydrogen ion concentration on lactate dehydrogenase(LDH)assays. Clin. Chem. 18: 1443-1449
  29. King, J. (1972) EIfect of hydrogen ion concentration on lactate dehydrogenase(LDH)assays. Clin. Chem. 18: 1443-1449
  30. Belcher, J. D. and Egan, J. O. (1991) A microenzymatic method to measure cholesterol and triglyceride in lipoprotein subfractions separated by density gradient ultracentrifugation from 200 microliters of plasma or serum. J. Lipid Res. 32: 359-370
  31. Uchiyama, M. and Mihara, M. (1978) Determination of malondialdehyde precursor in tissues by thiobarbituric acid test. Anal. Biochem. 86: 271-278 https://doi.org/10.1016/0003-2697(78)90342-1
  32. Ellman, G. L. (1959) Tissue sulfhydryl groups. Arch. Biochem. Biophy. 82: 70-77 https://doi.org/10.1016/0003-9861(59)90090-6
  33. Flohe, L., Wolfgng, A. and Gunzler, W. A. (1984) Assay of glutathione peroxidase. In Methods in enzymatic analysis, New York, Academic Press, Inc. 105: 114-121
  34. Habig, W. H., Pabst, M. J. and Jakoby, W. B. (1974) Glutathione S transferase, The first enzymatic step in mercapturic acid reaction. Anal. Biochem. 249: 7130-7139
  35. Cropo, C. H., McCord, J. M. and Fridovich, E. (1978) Preparation and assay of superoxide dismutase. In Methods in Enzymology, Fleischer, S. and Packer, L.(eds.), Academic Press, New York 52: 382-393
  36. Aebi, H. (1974) Catalase. In Methods of enzymatic analysis, Vergmeyer, H. U. (ed.), Academic Press. New York 2: 673-698
  37. Lowry, O. H., Rosebrough, N., Farr, A. L. and Randall, R. J. (1951) Protein measurement with folin phenol reagent. J. Biol. Chem. 193: 265-275
  38. Hayes, A. W. (1982) Principles and methods of toxicology. Raben Press, New York p407-445
  39. Hofmann, A. F. and Popper, H. (1987) Ursodeoxycholic acid for primary biliary cirrhosis. Lancet 2: 398-402
  40. Whitfield, J. B., Pounder, R. E., Neale, G. and Moss, D. W. (1972) Semrum $\gamma$-glutamyl transpeptidase activity in liver disease. Gut 13: 702-708 https://doi.org/10.1136/gut.13.9.702
  41. Curtis, M. T., Gilfor, D. and Farber, J. L. (1984) Lipid peroxidation increased the molecular order of microsomal membranes. Arch. Biochem. Biophy. 235: 644-651 https://doi.org/10.1016/0003-9861(84)90239-X
  42. Takeda, S., Funo, S., IIzuka, A., Kase, Y., Arai, I., Ohkura, Y., Sudo, K., Kiuchi, N., Yoshida, C., Maeda, S., Abrada, M. and Hosoya, E. (1985) Pharmacological studies on schizandra fruits. III. Effects of wuweizisu C, aligan component of schixanda fruits, on experimental liver injuries in rats. Folia Pharmacol. japan. 85: 194-208
  43. Noll, T. and Groot, H. (1984) The critical steady state hypoxic conditions in carbon tetrachloride induced lipid peroxidation in rat liver microsomes. Biochim. Biophys. Acta. 795: 356-362 https://doi.org/10.1016/0005-2760(84)90086-9
  44. Kim, H. C. and Hur, I. H. (1990) Antioxidant action of malotilate on prolonged hepatic injury induced by carbon tetrachloride alone or in combination with ethanol in rat. Yakhak Hoeji 34: 267-276
  45. Lim, H. K., Kim, H. S., Choi, H. S. and Choi, J. W.(1999) Protective and therapeutic effects of Malloti Cortex extract on carbon tetrachloride and galactosamine induced hepatotoxicity in rats. The Journal of Applied Pharmacology 7: 35-43
  46. Cohen, G. M. and Freedom, R. B. (1982) Roles and functions of glutathione. Biochem. Soc. Trans. 10: 78-85 https://doi.org/10.1042/bst0100078
  47. Meister, A. (1983) Selective modification of glutathione metabolism. Science 220: 472-477 https://doi.org/10.1126/science.6836290
  48. Vos, R. M. and Van Bladem, P. J. (1990) Glutathione S-transferase in relation to their role in the biotransformation of xenobiotics. Chem. Biol. Interact. 75: 241-265 https://doi.org/10.1016/0009-2797(90)90069-Y
  49. Crapo, H. C., McCord, M. J. and Fridovich, I. (1978) Preparation and assay of superoxide dismutase. In Methods in enzymology, Fleischer, S. and Packer, I. (eds.), Academic Press, New York 52: 382-393
  50. Deisseroth, A. and Dounce, A. L. (1970) Catalase physical and chemical properties, mechanism of catalysis and physiological role. Physiol. Rev. 50: 3-24
  51. Jones, D. R, Eklow, L., Thor, H. and Orrenius, S. (1981) Metabolism of hydrogen peroxide in isolated hepatocytes relative contributions of catalase and glutathione peroxidase in decomposition of endogenously generated $H_{2}O_{2}$. Arch. Biochem. Biophys. 210: 505-516 https://doi.org/10.1016/0003-9861(81)90215-0