Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Mutagenicity of the Essential Oils in Ames Test

Ames Test에 의한 정유의 돌연변이원성

  • 박희준 (상지대학교 응용식물과학부)
  • Published : 2002.12.30
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Abstract

Mutagenic acivity of essential oils was tested using Salmonella typhimurium TA100 in the presence or absence of 59 fraction prepared from the mouse liver. Growth inhibitory effect of the oils on the bacteria was measured to warrant the mutagenic effect. Most oils were (round to be very strongly toxic against the bacteria at a high dose $(2,000{\mu}g/plate)$. At lower doses than this concentration, the Curcuma longa oil was found to be the most mutagenic with S9 fraction whereas it was not mutagenic without the fraction suggesting that this oil could undergo activation for the mutagenicity by cytochrome P45O. However, the mutagenicity of the Eugenia caryohpylata oil was disappeared under S9 fraction. Other oils obtained from Cinnamomum cassia, Chrysanthemum sibiricum, Paeonia moutan the flower of Artemisia princeps var. Orientalis, Allium sativum, were not mutagenic. This result suggested that antimutagenicity assay on the essential oil is necessary for the biological available substances.

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References

  1. Park, H. J., Kwon S. H., Han Y. N., Choi J. W., Miyamoto K., Sung Ho Lee and Lee, K. T. (2001) Apoptosis-Inducing Costunolide and a Novel Monoterpene from the Stem Bark of Magnolia sieboldii, Arch. Pharm. Res. 24: 342-348 https://doi.org/10.1007/BF02975104
  2. Trump, B. F., Berezesky, I. K., Chang, S. H. and Phelps, P. C. (1997) The pathway ofcell death: oncosis, apoptosis, and necrosis, Toxicol. Pathol, 25: 82-88 https://doi.org/10.1177/019262339702500116
  3. Choi, J., Lee, K. T., Ka, H., Jung, W. T. and Park, H. J. (2001) Constituents of the essential oil of the Cinnamomumcassia stem bark and the biological properdes, Arch. Pharm, Res., 24: 418-423 https://doi.org/10.1007/BF02975187
  4. Park, H. J., Jung, W. T., Basnet, P., Kadota, S. and Namba, T. (1996) Syringin 4-O-glucoside, A New PhenylpropanoidGlycoside and Costunolide, Nitric Oxide Synthase Inhibitor from the Stem Bark of Magnolia Sieboldii, J. Nat. Prod. 59: 1128-1130 https://doi.org/10.1021/np960452i
  5. Beedam, C. (1997) The role of non-P450 enzymes in drug oxidation, Pharm. Sci. 19: 255-63
  6. Maron, D. M. and Ames, B. N. (1983) Revised methods for the Salmonella mutagenicity test. Mutat. Res. 113: 173-215 https://doi.org/10.1016/0165-1161(83)90010-9
  7. Chang, S. T., Chen, P. F. and Chang, S. C. (2001) Antibacterial activity of leaf essential oils and their constituents from Cinnamomomum osmophloeum, J. Ethnopharmacol. 77: 123-127 https://doi.org/10.1016/S0378-8741(01)00273-2
  8. 박희준, 권상혁, 이명선, 김갑태, 정원태(2000) 배초향 지상부에서 얻은 정유의 조성과 항균효과, 한국식품영양과학회지 29: 1123-1126
  9. Park, J. Y., Kwon, B. M., Chung, S. K. and Kim, J. H. (2001) Inhibitory effect of 2-O-benzoylcinnamaldehyde on vascular endothelial cell proliferation and migration, Ophthal. Res. 33: 111-116 https://doi.org/10.1159/000055654
  10. Lee, C. W., Hong, D. H., Han, S. B., Park, S. H., Kim, H. K., Kwon, B. M. and Kim, H. P. (1999) Inhibition of human tumor growth by 2-hydroxy- and 2-benzoylcinnamaldehydes, Planta Med. 65: 263-266 https://doi.org/10.1055/s-2006-960772
  11. Shaughnessy, D. T, Setzer, R. W. and DeMahni, D. M. (2001) The antimutagenic effect of vanillin and Cinnamal-dehyde on spontaneous mutation in Salmonella TA104 is due to a reduction in mutations at GC but not AT sites, Mut. Res., 480: 55-69
  12. Choi, J., Lee, K.T., Jung, W. T., Jung, H. J., Lee, S. H. and Park, H. J. (2002) Inhibitory effects of the essential oils on acetaminophen-induced lipid peroxidation in the rat, Nat. Prod. Sci. 8: 18-22