Korean Journal of Pharmacognosy (생약학회지)

The Korean Society of Pharmacognosy (한국생약학회)
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Effect of Daewhang-whangryunhaedok-Tang on Carbon Tetrachloride-induced Hepatotoxicity and Acute Toxicity

대황황련해독탕의 사염화탄소 유발 간장해 보호효과 및 급성독성

  • Kim, Young-Suk (College of Oriental Medicine, Kyung Hee University) ;
  • Jung, Eun-Ah (East-West Medical Research Institute, Kyung Hee University) ;
  • Chang, Jong-Chul (College of Oriental Medicine, Kyung Hee University) ;
  • Yang, Hyung-Kil (College of Oriental Medicine, Kyung Hee University) ;
  • Cho, Gi-Ho (College of Oriental Medicine, Kyung Hee University) ;
  • Bae, Hyung-Sup (College of Oriental Medicine, Kyung Hee University) ;
  • Lee, Kyung-Sup (College of Oriental Medicine, Kyung Hee University) ;
  • Kim, Dong-Hyun (College of Pharmacy, Kyung Hee University) ;
  • Kim, Nam-Jae (East-West Medical Research Institute, Kyung Hee University)
  • Published : 2002.03.30
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Abstract

This study was performed to evaluate hepatoprotective effect of Daewhang-whangryunhaedok-Tang(DWT) on liver injured rats induced by $CCl_4$ and the acute oral toxicity of it in mice. The activities of serum transaminase(ALT/AST), alkaline phosphatase(ALP) and lactic dehydrogenase (LDH), the levels of serum total cholesterol(TC) and triglyceride(TG), change of liver enlargement, and inhibitory activities of lipid peroxidation, catalase and glutathione-S-transferase(GST) in liver microsome were determined in hepatotoxic rats induced by $CCl_4$ DWT DWT was significantly reduced the serum ALT, AST, ALP, LDH, TC and TG levels. And, the increase of lipid peroxidation, decrease of catalase and GST activities in the liver microsome of $CCl_4$-intoxicated rat were significantly improved by the treatment of DWT. Male and female mice were administered maximum dosages of 5,000 mg/kg b.w. of DWT. After single oral administration of DWT to mice, we observed them daily for 2 weeks. DWT did not induce any toxic signs in the mortalities, clinical signs, body weight changes, and gross necropsy findings of mice. Based on these results, it is concluded that DWT may have the hepatoprotective effect on $CCl_4$ induced hepatotoxicity in rats. Also, DWT may have no side effect and its $LD_{50}$ value may be over 5,000 mg/kg b.w. in mice.

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References

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