Journal of Oriental Neuropsychiatry (동의신경정신과학회지)
- Volume 13 Issue 2
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- Pages.195-211
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- 2002
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- 1226-6396(pISSN)
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- 2234-4942(eISSN)
Experimental Study on the Sedative Effect of Gagamguibitang
가감귀비탕(加減歸脾湯)의 진정 효과에 대한 실험적 연구
- Kim, In-Jae (Department of Neuropsychiatry, College of Oriental Medicine, Dongshin University) ;
- Lee, Dong-Won (Department of Neuropsychiatry, College of Oriental Medicine, Dongshin University) ;
- Ryu, Jong-Sam (Department of Internal Medicine, College of Oriental Medicine, Dongshin University) ;
- Hong, Seok (Department of Internal Medicine, College of Oriental Medicine, Dongshin University) ;
- Kim, Eun-Jung (Department of Neuropsychiatry, College of Oriental Medicine, Dongshin University)
- 김인재 (동신대학교 한의과대학 신경정신과학교실) ;
- 이동원 (동신대학교 한의과대학 신경정신과학교실) ;
- 류종삼 (동신대학교 한의과대학 내과학교실) ;
- 홍석 (동신대학교 한의과대학 내과학교실) ;
- 김은정 (동신대학교 한의과대학 신경정신과학교실)
- Published : 2002.12.31
Abstract
Gagamguibitang, a composite Korean medicinal drug prescribed by us, was evaluated for its sedative effects by measurements of potentiation on pentobarbital-induced sleeping time, anticonvulsive activities in animal model, inhibitory effect on GABA transaminase activity, and antioxidative activities in vitro- and/or in vivo assay. The results were summerized as follows : 1. Gagamguibitang showed about 2-fold prolongation of pentobarbital-induced sleeping time compared to the control group after administration(p.o) with 2.0g/kg of mice body weight. 2. Gagamguibitang strongly lengthened onset time of pentylenetetrazole-induced convulsion, shortened the duration of convulsion and diminished the lethality after treatment(p.o) with 1.0g/kg of mice body weight. 3. Gagamguibitang inhibited dose-dependently the brain GABA transaminase activity in vitro compared to the control group and in vivo compared to the pentylenetetrazole-treated group. 4. Gagamguibitang inhibited effectively brain lipid peroxidation by 45.8% at a dose of l0mg/ml in vitro and by 47.5% after oral treatment with 0.5g/kg of mice body weight in vivo assay. 5. Gagamguibitang exhibited a potent scavenging activity on DPPH radical in a dose-dependent manner with ca. 92% activity at l0mg/ml. As a result, Gagamguibitang can be useful for the effective sedative drug in clinical application.
Keywords
- Gagamguibitang;
- sedation;
- sleep;
- anticonvulsion;
- GABA transaminase;
- antioxidation;
- lipid peroxidation;
- free radical