Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
권호 표지
DOI QR코드

DOI QR Code

Development of an ELISA for the Organophosphorus Insecticide Isofenphos

  • Published : 2002.04.20
Download PDF
⟨ Previous Next ⟩

Abstract

A selective enzyme-linked immunosorbent assay (ELISA) for the insecticide isofenphos was developed. Three different analogues (haptens) of isofenphos were synthesized and were coupled to carrier proteins through the pesticide thiophosphate group t o use as immunogens or coating antigens. Rabbits were immunized with one of the haptens coupled to BSA for production of polyclonal antibodies and the sera were screened against each of the other two haptens coupled to ovalbumin (OVA). Using the sera of highest specificity, an antigen-coated ELISA was developed, which showed an I50 of 96 ng/mL with the detection limit of 2 ng/mL. The antibodies showed negligible cross-reactivity with other organophosphorus pesticides and the phenol metabolite of isofenphos, which makes the developed assay suitable for the selective detection of isofenphos. An antibody-coated ELISA was also developed, which showed an I50 of 580 ng/mL with a detection limit of 70 ng/mL.

Keywords

References

  1. AOAC International Official Methods of Analysis, 18th Ed.;Arlington, VA, 1995; Section 970.52.
  2. Hammock, B. D.; Gee, S. J.; Harrison, R. O.; Jung, F.; Goodrow, M. H.; Li, Q. X.; Lucas, A.; Szekacs, A.; Sundaram, K. M. S. Immunochemical Methods for Environmental Analysis; Van Emon, J., Mumma, R., Eds.; ACS Symposium Series 442; American Chemical Society: Washington, DC, USA, 1990; pp 112-139.
  3. The Pesticide Manual: A World Compendium, 11th ed.; Tomlin,C. D. S., Ed.; The British Crop Protection Council: Farnham,Surrey, U.K., 1997; p 238.
  4. McAdam, D. P.; Hill, A. S.; Beasley, H. L.; Skerritt, J. H. J. Agric.Food Chem. 1992, 40, 1466-1470. https://doi.org/10.1021/jf00020a035
  5. Manclús, J. J.; Primo, J.; Montoya, A. J. Agric. Food Chem. 1996,44, 4052-4062. https://doi.org/10.1021/jf960144q
  6. Skerritt, J. H.; Lee, N. J. Residue Analysis in Food Safety: Applications of Immunoassay Methods.; Beier R. C., Stanker, L. H., Eds., ACS Symposium Series 621; American Chemical Society: Washington DC, USA, 1996; pp 124-149.
  7. Raab, G. M. Clin. Chem. 1983, 29, 1757-1761.
  8. Gee, S. J.; Miyamoto, T.; Goodrow, M. H.; Buster, D.; Hammock,B. D. J. Agric. Food Chem. 1988, 36, 864-870.
  9. Harrison, R. O.; Goodrow, M. H.; Hammock, B. D. J. Agric. Food Chem. 1991, 39, 122-128. https://doi.org/10.1021/jf00001a024
  10. Kido, H.; Goodrow, M. H.; Griffeth, V.; Lucas, A. D.; Gee,S. J.; Hammock, B. D. J. Agric. Food Chem. 1997, 45, 414-424. https://doi.org/10.1021/jf960164s
  11. Shan, G.; Leeman, W. R.; Stoutamire, D. W.; Gee, S. J.; Chang,D. P. Y.; Hammock, B. D. J. Agric. Food Chem. 2000, 48, 4032-4040. https://doi.org/10.1021/jf000351x
  12. Oubina, A.; Ballesteros, B.; Galve, R.; Barcelo, D.; Marco, M.-P.Anal. Chim. Acta 1999, 387, 255-266. https://doi.org/10.1016/S0003-2670(98)00775-2
  13. Oubina, A.; Barcelo, D.; Marco, M.-P. Anal. Chim. Acta 1999,387, 267-279. https://doi.org/10.1016/S0003-2670(98)00826-5
  14. Jefferis, R.; Deverill, I. Principles and Practice of Immunoassay;Price, C. P., Newman, D. J., Eds.; Stockton Press: New York,1991; Chapter 1.
  15. Hennion, M.-H.; Barcelo, D. Anal. Chim. Acta 1998, 362, 3-34. https://doi.org/10.1016/S0003-2670(97)00608-9

Cited by

  1. Development of a dipstick enzyme-linked immunosorbent assay for the organophosphorus insecticide parathion-methyl vol.41, pp.8, 2006, https://doi.org/10.1111/j.1365-2621.2005.01144.x
  2. Development of an Enzyme-Linked Immunosorbent Assay for the Organophorus Insecticide Bromophos vol.23, pp.10, 2002, https://doi.org/10.5012/bkcs.2002.23.10.1399
  3. Development of an Enzyme-Linked Immunosorbent Assay for the Organophosphorus Fungicide Tolclofos-methyl vol.24, pp.3, 2002, https://doi.org/10.5012/bkcs.2003.24.3.334
  4. Monoclonal antibody-based enzyme-linked immunosorbent assays for the detection of the organophosphorus insecticide isofenphos vol.557, pp.1, 2002, https://doi.org/10.1016/j.aca.2005.10.007
  5. Development of an enzyme-linked immunosorbent assay for detection of Chlorpyrifos-ethyl and its metabolites 3,5,6-Trichloro-2-Pyridinol and Diethylthiophosphate vol.100, pp.12, 2002, https://doi.org/10.1080/03067319.2019.1653456