Bulletin of the Korean Chemical Society

Korean Chemical Society (대한화학회)
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Enhanced UV-Visible Absorbance Detection in Capillary Electrophoresis Using Modified T-Shaped Post-Column Flow Cell

  • Lim, Kwan-Seop (National Research Laboratory for Advanced Biotechnology and Biomedical Microinstrumentation, Division of Molecular and Life Sciences, Pohang University of Science and Technology) ;
  • Kim, Su-Hyeon (LG Electronic Institute of Technology) ;
  • Hahn, Jong-Hoon (National Research Laboratory for Advanced Biotechnology and Biomedical Microinstrumentation, Division of Molecular and Life Sciences, Pohang University of Science and Technology)
  • Published : 2002.02.20
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Abstract

The construction of the T-shaped post-column flow cell has been changed to enhance the practicability as a UV-visible absorbance detector for capillary electrophoresis. In this new design, a rectangular cube-shaped inner structure is employed, which completely fits the outer rectangular tubing. This arrangement has greatly facilitated the fabrication of the T-cells. In addition, the volume for the auxiliary flow has been dramatically reduced down to 300 ${\mu}L$, and its volume flow rate is optimized at 4.2 ${\mu}L$/min. The short optical path length in the sheath flows (500 ${\mu}m$ on each side) minimizes background absorption, and thus enhances its performance in low-UV wavelengths. We have optimized the auxiliary flow rate at 50 ${\mu}m$/s, so that migration times are insensitive to the flow rate. This optimization has improved repeatabilities in migration times and peak heights. A double-beam detection scheme using a pair of photodiodes is employed to increase the signal-to-noise ratio.

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