Journal of Radiation Protection and Research

The Korean Association for Radiation Protection (대한방사선방어학회)
권호 표지

Protective Effects of Paeonia japonica against Radiation-induced Damage

방사선 장해에 대한 백작약의 방호효과

  • Oh, Heon (Radiation Food Technology and Bioscience team, Korea Atomic Energy Research Institute) ;
  • Park, Hae-Ran (Radiation Food Technology and Bioscience team, Korea Atomic Energy Research Institute) ;
  • Jeong, Ill-Yun (Radiation Food Technology and Bioscience team, Korea Atomic Energy Research Institute) ;
  • Kim, Sung-Ho (College of Veterinary medicine, Chonnam National University) ;
  • Jo, Sung-Kee (Radiation Food Technology and Bioscience team, Korea Atomic Energy Research Institute)
  • 오헌 (한국원자력연구소 방사선식품 생명공학기술 개발팀) ;
  • 박혜란 (한국원자력연구소 방사선식품 생명공학기술 개발팀) ;
  • 정일윤 (한국원자력연구소 방사선식품 생명공학기술 개발팀) ;
  • 김성호 (전남대학교 수의과대학) ;
  • 조성기 (한국원자력연구소 방사선식품 생명공학기술 개발팀)
  • Published : 2002.09.30
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Abstract

We investigated the effect of Paeonia japonica (PJ) on radiation-induced oxidative damage to macromolecules in vitro and in vivo. The PJ reduced the tail moment (TM) which was a marker of DNA strand break in single-cell gel electrophoresis (SCGE; comet assay) in the human peripheral blood lymphocytes. Lipid peroxidation in the liver of the ICR mouse, measured as malondialdehyde (MDA), was also reduced by PJ administration. Ethanol fraction of PJ was more effective than polysaccharide fraction of that on reduction of TM in SCGE and lipid peroxidation. Also, Their activities to scavenge DPPH radicals and hydroxyl radicals were observed in vitro, and the activities were due to its ethanol fraction. It is plausible that scavenging of flee radicals by PJ extract may have played an important role in providing the protection against the radiation-induced damage. These results indicated that Paeonia japonica might be a useful radioprotector, especially since it is a relatively nontoxic natural product.

방사선 생체 손상에 대한 방호 효과를 나타내는 천연물을 검색하기 위한 일환으로 한의학에서 보혈양혈 탕제에 널리 사용되는 백작약 (Paeonia japonica)을 열수총추출물, 에탄올분획, 조다당분획으로 나누어 방사선에 의한 산화적 손상 경감 효과를 검정하였다. 사람 림프구에서 단세포전기영동 (single cell gel electrophoresis; comet assay)을 수행하여 DNA 손상 경감정도를 관찰하였으며, 마우스에 백작약 추출물을 투여한 다음 8 Gy의 감마선을 조사한 후 간에서 지질과산화 정도를 살펴보았다. 에탄올분획 처리군에서 높은 DNA 손상 경감효과를 확인할 수 있었으며, 지질과산화 억제작용 및 라디칼 소거효과 또한 에탄올분획이 높은 효과를 나타내어 에탄올분획이 방사선 방호에 주된 역할을 하는 것으로 사료된다. 이상의 결과로 보아 백작약은 방사선의 산화절 손상에 대하여 효과적으로 세포 DNA를 방호하고, 생체막의 주성분인 지질의 과산화를 억제하는 것으로 관찰되어 특히, 독성이 거의 없는 천연물이라는 관점에서 방사선 방호제로 적용이 가능할 것으로 사료된다.

Keywords

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