Journal of Sasang Constitutional Medicine (사상체질의학회지)

The Society Of Sasang Constitutional Medicine (사상체질의학회)
권호 표지

Studies on the Cytokine Production Regulation in Human Astrocytes by Yuldahansotang

인간뇌성상세포(人間腦星狀細胞)에서 열다한소탕(熱多寒少湯)에 의한 세포활성물질(細胞活性物質) 생성(生成) 조절(調節)에 관(關)한 연구(硏究)

  • Choi, Ji-sook (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Kyoung-yo (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.) ;
  • Kim, Hyoung-min (College of Oriental Medicine, Wonkwang Univ.) ;
  • Ju, Jong-chon (Dept. of Sasang Constitutional medicine, College of Oriental Medicine, Wonkwang Univ.)
  • 최지숙 (원광대학교한의과대학 사상체질과) ;
  • 김경요 (원광대학교한의과대학 사상체질과) ;
  • 김형민 (원광대학교 한의과대학) ;
  • 주종천 (원광대학교한의과대학 사상체질과)
  • Published : 2000.12.31
Download PDF
⟨ Previous Next ⟩

Abstract

사상의학적 견지에서 태음인(太陰人)의 중풍, 치매와 같은 신경계질환에 다용되고 있는 열다한소탕(熱多寒少湯)은 최근에 그 임상적 효과를 뒷받침할 다각적인 연구들이 이루어지고 있음에도 불구하고 그 정확한 약리학적 기전에 대해서는 밝혀지지 않고 있다. 본 연구에서는 인간성상세포를 이용하여 열다한소탕(熱多寒少湯)이 substance P (SP)와 lipopolysaccharide (LPS)에 의해 유도되는 다양한 세포활성물질의 분비량의 조절을 검토함으로써 열다한소탕(熱多寒少湯)의 약리기전을 면역학적 측면에서 보다 세밀하게 살펴보고자 하였다. 열다한소탕(熱多寒少湯) 수침액은 인간 뇌 성상세포로 부터 LPS와 SP의 동시자극에 의해 생성되는 세포활성물질중 interleukin (IL)-1, IL-4, IL-6 및 tumor necrosisfaccor-${\alpha}$ (TNF-${\alpha}$)의 분비를 농도의존적으로 억제했다. 그러나 interferon-${\gamma}$ (IFN-${\gamma}$) 및 IL-2의 분비 조절에는 영향을 미치지 않았다. 그리고 항 IL-$1{\beta}$ 항체에 의해 SP 유도성 TNF-${\alpha}$ 분비의 증가가 억제되기 때문에 IL-1은 TNF-${\alpha}$ 증가를 매개하는 역할을 하는 것으로 사료된다. 이상의 결과는 열다한소탕(熱多寒少湯)에 의한 급성기 중풍환자 치료 효과가 뇌 성상세포로부터 분비되는 세포활성물질의 조절과 밀접한 관련성이 있다는 것을 암시하고 있다.

Keywords

References

  1. J Sasang Constitutional Med v.3 no.1 A study on the change of blood constituent in male student of TAE-EUM-IN. Kim, K.Y.
  2. J Sasang Constitutional Med v.5 no.1 An experimental study on the analysis of the constituents of serum lipid classified by constitutions. Kim, D.R.
  3. J Sasang Constitutional Med v.10 no.1 Effects of Yuldahansotang water extrat on blood pressure, regional cerebral blood flow and pial artery diameter. Choi, Y.J.
  4. J Sasang Constitutional Med v.10 no.2 Effects of Yuldahansotang and Jowisok-myungtang on the blood pressure and renal function in spontaneously hypertensive rats. Yun, H.S.
  5. J Sasang Constitutional Med v.11 no.2 Effects of Yuldahansotang after kainate administration in the mouse hippocampus area. Kim, I.H.;Kim, K.Y.
  6. J Sasang Constitutional Med v.12 no.1 Studies on the regulatory effect of cytokine production in patients with cerebral infarction by Yuldahansotang. Choi, Y.K.;Kim, K.Y.
  7. Immunol v.100 Immune-mediated encephalitis; on the role of antigen-presenting cells in brain tissue. Fontana, A.;Frei, K.;Bodmer, S.;Hofer, E.
  8. J Neuroimmunol v.30 Induction regulation of interleukin-6 gene expression in rat astrocytes. Benveniste, E.N.;Saparcio, S.M.;Norris, J.G.;Grenett, H.E.;Fuller, G.M.
  9. J Immunol v.144 Production of hemopoietic colony-stimulating factors by astrocytes. Malipiero, U.V.;Frei, K.;Fontana, A.
  10. Int J Dev Neurosci v.13 no.3 Cytokine regulation of astrocyte function: in-vitro studies using cells from the human brain. Aloisi, F.;Borsellino, G.;Testa, U.;Gallo, P.;Russo, G.;Peschle, C.;Levi, G.
  11. Neuroscience Lett v.129 Elevated circulating tumor necorsis factor levels in Alzheimer's disease. Fillit, H.;Ding, W.H.;Buce, L.;Kalman, J.;Altstiel, L.;Lawlor, B.;Wolf-Klein, G.
  12. J Neuroimmunol v.18 Hypothesis: a role for tumor necrosis factor in immune-related demyelination and its relevance to multiple sclerosis. Brosnan, C.F.;Selmaj, F.K.;Raine, C.S.
  13. J Neuroimmunol v.30 Astrocyte conditioned medium stimulates HIV-1 expression in a chronically infected promonocyte clone. Vitkovic, L.;Kalevic, T.;de Cunha, A.;Fauci, A.S.
  14. J Mol Neurosci v.14 Reduced IL-2 but elevated IL-4, IL-6, and IgE serum levels in patients with cerebral infarction during the acute stage. Kim, H.M.;Shin, H.Y.;Jeong, H.J.(et al.)
  15. Neuroscience v.3 Distribution of substance P-like immuno-reactivity in the central nervous system of the rat-I. Cell bodies and nerve terminals. Ljungdahl, A.;Hokfelt, T.;Nilsson, G.
  16. Naunym-Schmieddeberg's Arch Pharmacol v.310 Substance P as neurogenic mediator of antidromic vasodilation and neurogenic plasma extravasation. Lembeck, F.;Holzer, P.
  17. Science v.241 Effect of neuropeptides on production of inflammatory cytokines by human monocytes. Lotz, M.;Vaughan, J.H.;Carson, D.A.
  18. Scand J Immunol v.31 The neuropeptide substance P stimulates production of interleukin-1 in human blood monocytes: activated cells are preferentially influenced by the neuropeptide. Laurenzi, M.A.;Persson, M.A.A.;Dalsgaard, C.J.;Haegerstrand, A.
  19. USA Proc Natl Acad Sci v.86 Substance P receptor binding sites are expressed by glia in vivo after neuronal injury. Manthy, P.W.;Johnson, D.J.;Boehemer, C.G.;Catton, M.D.;Vinters, H.V.;Maggio, J.E.;Too, H.P.;Vigna, S.R.
  20. Lancet v.2 Raised serum levels of tumour necrosis factor in parasitic infections. Scuderi, P.;Sterling, K.E.;Lam, K.S.;Finley, P.R.;Ryan, K.J.;Ray, C.G.;Petersen, E.;Slymen, D.J.;Salmon, S.E.
  21. Zinsser Microbiology. Joklik, W.K.;Willett, H.P.;Amos, D.B.;Wilfert, C.M.
  22. FEBS Lett v.399 no.3 Effect of substance P on cytokine production by human astrocytic cells and blood mononuclear cells: characterization of novel tachy-kinin receptor antagonists. Derocq, J.M.;Blazy, C.;Emonds, A.X.;Le, F.G.;Brelire, J.C.;Casellas, P.
  23. Korean translation ed. of the 3rd ed. Ha, D.Y.;Ivan, R.;Jonathan, B.;David, M.
  24. J Neuroimmunol v.101 no.1 Potential mechanisms of interleukin-1 involvement in cerebral ischemia. Touzani, O.;Boutin, H.;Chuquet, J.;Rothwell, N.
  25. Keio J Med v.45 no.3 Involvement of cytokine prodiction in pathogenesis of transient cerebral ischemic damage. Yamasaki, Y.;Itoyama, Y.;Kogure, K.
  26. Stroke v.28 no.6 Tumor necrosis factor-alpha. A mediator of focal ishemic brain injury. Barone, F.C.;Arvin, B.;White, R.F.;Miller, A.;Webb, C.L.;Willette, R.N.Lysko, P.G.;Feuerstein, G.Z.
  27. J Cereb Blood Flow Metab v.20 no.6 Ischemia-induced interleukin-6 as a potential endogenous neuroprotective cytokine against NMDA receptor-mediated excitotoxicity in the brain. Ali, C.;NIcole, O.;Docagne, F.;Lesne, S.;Mackenzie, E.T.;Nouvelot, A.;Buisson, A.;Vivien, D.
  28. J Sasang Constitutional Med v.7 no.2 The Sasang Constitutional Approach to an Incurable Disease and Immunity. Kim, K.Y.
  29. Donwi Soose Bowon Lee, J.M.