IMMUNE NETWORK
- Volume 1 Issue 2
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- Pages.109-115
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- 2001
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
Interferon consensus sequence binding protein : Not essential for interferon α-mediated antiviral response to vesicular stomatitis virus infection in HL-60 cells
- Park, Byung-Kiu (Department of Pediatrics, Gyeongsang National University College of Medicine and Gyeongsang Institute of Cancer Research)
- Published : 2001.08.31
Abstract
Background: The role of the interferon consensus sequence binding protein (ICSBP), a member of interferon regulatory factor family, in protecting against a vesicular stomatitis virus (VSV) infection has not been firmly elucidated. Thus, it was investigated utilizing the human promyelocytic leukemia HL-60 cells which do not express ICSBP. Methods: HL-60 cells were stably transfected with plasmid containing cDNA for either ICSBP or DNA binding domain (DBD) and tested for their VSV-susceptibilities. The susceptibility of each transfectant group to a VSV infection was determined by a plaque assay at 1 h, 24 h, and 48 h post-infection in the presence (500 IU/ml) or absence of interferon
Keywords
- interferon consensus sequence binding protein;
- DNA binding domain;
- interferon ${\alpha}$;
- vesicular stomatitis virus infection