IMMUNE NETWORK
- 제1권1호
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- Pages.70-76
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- 2001
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- 1598-2629(pISSN)
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- 2092-6685(eISSN)
류마티스양 관절염 환자 활막 T 세포의 T 세포수용체 β쇄 분석
Usage of T Cell Receptor Repertoire is Restricted in Synovial Lymphocytes in Rheumatoid Arthritis
- 권대호 (연세대학교 의과대학 미생물학교실 및 면역질환연구소) ;
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이수곤
(연세대학교 의과대학 내과학교실) ;
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김세종
(연세대학교 의과대학 미생물학교실 및 면역질환연구소) ;
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최인홍
(연세대학교 의과대학 미생물학교실 및 면역질환연구소)
- Kwon, Dae-ho (Department of Microbiology and Institute for Immunology and Immunological Diseases) ;
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Lee, Soo-Kon
(Department of Internal Medicine, Yonsei University College of Medicine) ;
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Kim, Se-Jong
(Department of Microbiology and Institute for Immunology and Immunological Diseases) ;
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Choi, In-Hong
(Department of Microbiology and Institute for Immunology and Immunological Diseases)
- 발행 : 2001.04.30
초록
Background: Rheumatoid arthritis is an autoimmune disease characterized by a chronic inflammatory process, primarily involving the synovial membrane of peripheral j oints, where T cell activation is found. To address the superantigen stimulation in rheumatoid arthritis, T cell clonality and the expression of activation markers were analyzed. Methods: To detect TCRB V usage, inverse PCR and sequencing were done. Monoclonal antibodies were used for flow cytometric analysis of TCRBV8 or TCRBV5. As results, a restricted usage of TCRBV3 gene was detected in synovial lymphocytes from one rheumatoid arthritis patient. However, preferential usage for TCRB V8, which may be one indicator for stimulation by staphylococcal superantigen, was not obvious although general activation of T cells was found as high DR+ percentage in synovial T cells. These data show specific antigen rather than superantigen might involve the pathogenesis of rheumatoid arthritis.