Determination of the Temperature-Dependent Index of Mitotic Interval (${\tau}_o$) for Chromosome Manipulation in Far Eastern Catfish Silurus asotus

메기 Silurus asotus 염색체조작을 위한 온도 의존적 체세포분열 간격지수 결정

  • Park, In-Seok (Division of Ocean Science, College of Ocean Science and Technology, Korea Maritime University) ;
  • Im, Jae-Hyun (Faculty of Marine Life Science, Kunsan National University)
  • 박인석 (군산대학교 해양생명과학부) ;
  • 임재현 (군산대학교 해양생명과학부)
  • Received : 2000.10.04
  • Accepted : 2000.11.25
  • Published : 2001.03.28

Abstract

Temperature-related cleavage rates and mitotic intervals (${\tau}_o$) in the far eastern catfish Silurus asotus were studied by averaging the duration of the second and third embryonic divisions to establish effective procedures for chromosome manipulations. At higher temperatures eggs developed faster and underwent more asynchronous development. Mitotic intervals for far eastern catfish were $21.5{\pm}1.4$ min at $22^{\circ}C$, $18.5{\pm}1.2$ min at $24^{\circ}C$, and $14.0{\pm}2.1$ min at $26^{\circ}C$. There was a strong negative correlation between ${\tau}_o$ and water temperature (Y=-1.85X+21.9, $R^2=0.9868$, where Y is ${\tau}_o$ and X is temperature).

메기 Silurus asotus에서 염색체조작을 효과적으로 하기 위하여, 여러 온도 조건에서의 난할율과 제2, 제3 난할 시간의 평균화에 의한 체세포분열 간격지수 (${\tau}_o$)를 조사하였다. 빠른 난 발생과 비동시적 발생은 고온에서 더욱 뚜렷하게 나타났다. 메기의 체세포분열 간격지수는 $22^{\circ}C$에서 $21.5{\pm}1.4$분, $24^{\circ}C$에서 $18.5{\pm}1.2$분 그리고 $26^{\circ}C$에서 $14.0{\pm}2.1$분이었다. 3가지 수온조건과 ${\tau}_o$ 간에는 Y=-1.85X+21.9 ($R^2=0.9868$, Y는 ${\tau}_o$ 그리고 X는 수온)의 직선회귀가 성립되었다.

Keywords