Korean Journal of Fisheries and Aquatic Sciences (한국수산과학회지)

The Korean Society of Fisheries and Aquatic Science (한국수산과학회)
권호 표지

Purification of Myomodulin A and Myomodulin E from the Central Nervous System of the Sea Hare, Aplysia kurodai

군소 (Aplysia kurodai)의 중추신경계로부터 myomodulin A와 E의 정제

  • KIM Chan-Hee (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • SEO Hae Jeom (Department of Food and Life Science, College of Fisheries Science, Pukyong National University) ;
  • HWANG Eun-Young (Department of Food and Life Science, College of Fisheries Science, Pukyong National University) ;
  • KIM Eun Jung (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • GO Hye-Jin (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • KIM In Hye (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • Seo Jung-Kil (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University) ;
  • Moon Jung-Hye (Seafood and Marine Resources Development Center) ;
  • HUH Min-Do (Department of Aquatic Life Medicine, College of Fisheries Science, Pukyong National University) ;
  • PARK Nam Gyu (Department of Biotechnology and Bioengineering, College of Fisheries Science, Pukyong National University)
  • 김찬희 (부경대학교 수산과학대학 생물공학과) ;
  • 서해점 (부경대학교 식품생명과학과) ;
  • 황은영 (부경대학교 식품생명과학과) ;
  • 김은정 (부경대학교 수산과학대학 생물공학과) ;
  • 고혜진 (부경대학교 수산과학대학 생물공학과) ;
  • 김인혜 (부경대학교 수산과학대학 생물공학과) ;
  • 서정길 (부경대학교 수산과학대학 생물공학과) ;
  • 문정혜 (해양식량자원특성화사업단) ;
  • 허민도 (부경대학교 수산생명의학과) ;
  • 박남규 (부경대학교 수산과학대학 생물공학과)
  • Published : 2001.05.01
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Abstract

This paper reports the purification of myomodulin A (MMA) and myomodulin E (MME) from the sea hare. The central nervous systems of 500 sea hare were extracted in an acidified solvent, after which four HPLC column systems were used to obtain pure peptides, The phasic contraction bioassay using a Mytilus edulis anterior byssus retractor muscle (ABRW) was applied to monitor all collected fractions. The pure peptides were submitted to Edman degradation based automated microsequencing. Mass spectrometry and chemical synthesis confirmed the sequence. The primary structures of MMA and MME were Pro-Met-Ser-Met-Leu-Arg-Leu-$NH_2$, (847.41 Da) and Cly-Leu-Gln-Met-Leu-Arg-Leu-$NH_2$, (830.50 Da), respectively. Synthetic peptides showed a modulating activity of phasic contraction in the ABRM of Mrtilus edulis.

군소의 중추신경계로부터 myomodulin A (MMA)과 myomodlin E (MME)의 정제에 대해 보고하고자 한다. 500마리 군소의 중추신경계를 산조건으로 추출한 후 4종류의 HPLC column system에 적용하여 두가지 신경성 펩타이드를 정제하였다. HPLC 분리과정에서 얻어진 모든 분획의 활성을 조사하기 위하여 bioassay system으로 진주담치 (Mytilis edulis)의 ABRM을 이용하였다. 최종 정제된 신경성 펩타이드는 Edman 분해법을 이용한 아미노산 서열 분석기, 질량분석기와 화학합성을 통해 완전한 일차구조를 밝힐 수 있었다. 정제한 MMA와 MME의 일차구조는 각각 Pro-Met-Ser-Met-Leu-Arg-Leu-$NH_2$, (847.41 Da) 과 Gly-Leu-Gln-Met-Leu-Arg-Leu-$NH_2$, (830.50Da) 이었다. 또한 이들 합성 펩타이드는 진주담치의 ABRM에 대해 phasic contration의 조절활성을 나타내었다.

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