Journal of Preventive Medicine and Public Health
- Volume 34 Issue 1
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- Pages.41-46
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- 2001
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- 1975-8375(pISSN)
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- 2233-4521(eISSN)
Effects of Particulate Matters on A549 and RAW 264.7 Cells
대도시의 입자상 물질이 A549와 RAW 264.7 세포에 미치는 영향
- Baak, Young-Mann (Department of Occupational and Environmental Medicine, St. Mary's Hospital, The Catholic University of Korea) ;
- Kim, Ji-Hong (Department of Occupational and Environmental Medicine, St. Mary's Hospital, The Catholic University of Korea) ;
- Kim, Kyoung-Ah (Department of Occupational and Environmental Medicine, St. Mary's Hospital, The Catholic University of Korea) ;
- Ro, Chul-Un (Department of Chemistry, Hallym University) ;
- Kim, Hyung-Jung (Department of Internal Medicine, College of Medicine, Yonsei University) ;
- Lim, Young (Department of Occupational and Environmental Medicine, St. Mary's Hospital, The Catholic University of Korea)
- 박영만 (가톨릭대학교 성모병원 산업의학과) ;
- 김지홍 (가톨릭대학교 성모병원 산업의학과) ;
- 김경아 (가톨릭대학교 성모병원 산업의학과) ;
- 노철언 (한림대학교 화학과) ;
- 김형중 (연세대학교 의과대학 내과학교실) ;
- 임영 (가톨릭대학교 성모병원 산업의학과)
- Published : 2001.03.01
Abstract
Objectives : To investigate the effects of particulate matter (PM), a marker of environmental pollution derived from combustion sources, on lung epithelial cells (A549) and macrophage (RAW 264.7). Methods : The production of reactive radicals from lung cells, the lipid peroxidation of cell membrane, and the cytotoxicity of PM were measured using an in vitro model. The results were compared with a control group. Results : The presence of PM significantly increased the production of reactive oxygen species and reactive nitrogen species with time and in a dose dependent pattern and also increased the malondialdehyde concentration in lung epithelial cells. The cytotoxicity of PM was increased with increasing concentration of PM. Conclusions : It has been suggested that urban particulate matter causes an inflammatory reaction in lung tissue through the production of hydroxyl radicals, nitric oxides and numerous cytokines. The causal chemical determinant responsible for these biologic effects are not well understood, but the bioavailable metal in PM seems to determine the tonicity of inhaled PM.