Journal of Applied Biological Chemistry
- Volume 44 Issue 2
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- Pages.92-94
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- 2001
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- 1976-0442(pISSN)
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- 2234-7941(eISSN)
Simple Detection of Opines by Paper Electrophoresis for Hairy Roots Transformed with Agrobacterium rhizogenes Strains
- Cho, Hyeon-Je (Department of Crop Sciences, University of Illinois) ;
- Ha, Hyo-Cheol (Department of Food Science and Technology, Yeungnam University) ;
- Lee, Jae-Sung (Department of Food Science and Technology, Yeungnam University) ;
- Widholm, Jack M. (Department of Crop Sciences, University of Illinois)
- Received : 2001.03.20
- Published : 2001.06.30
Abstract
A simple protocol for the detection of opines, cucumopines and mikimopines using a general horizontal or vertical get electrophoresis system for protein or DNA separation in the laboratory are demonstrated. This electrophoresis method can also be applied to other opines as long as correct detection reagent and buffer system are used.