Development and Reproduction (한국발생생물학회지:발생과생식)
- Volume 5 Issue 1
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- Pages.47-52
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- 2001
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- 2465-9525(pISSN)
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- 2465-9541(eISSN)
Discontinuous Percoll Gradients Enrich X-Bearing Porcine Sperms and Female Embryos
불연속 Percoll 원심분리에 의한 돼지 X-정자와 자성배아에 관한 연구
- Shim, Dae-Yong (Department of Biology, College of Natural Sciences, Inje University) ;
- Yoo, Seong-Jin (Department of Biology, College of Natural Sciences, Inje University) ;
- Kang, Han-Seung (Department of Biology, College of Natural Sciences, Inje University) ;
- Yoo, Jeong-Min (Department of Biology, College of Natural Sciences, Inje University) ;
- Lee, Chae-Kwan (Department of Biology, College of Natural Sciences, Inje University) ;
- Kang, Sung-Goo (Department of Biology, College of Natural Sciences, Inje University)
- 심대용 (인제대학교 생물학과) ;
- 유성진 (인제대학교 생물학과) ;
- 강한승 (인제대학교 생물학과) ;
- 유정민 (인제대학교 생물학과) ;
- 이채관 (인제대학교 생물학과) ;
- 강성구 (인제대학교 생물학과)
- Published : 2001.06.30
Abstract
Predetermination of sex in livestock of offpring is in great demand and is of critical importance to providing for the most efficient production of the animal ariculture. Such a sexing techlology would also enhance the economy of conventional artificial insemination(AI) and aid the porcine industry. The purpose of this study was to evaluate the efficiency of enriching X-bearing porcine sperm using discontinuous percoll gradients and PCR mefhod. Semen was collected from mature boars of proven fertility center (AI center KimHae). Sperm was leaded on the isotonic discontinuous percoll gradient and then it was centrifuged at 120
X, Y정자를 분리하여(sexing techlology)필요한 성을 조절한 배아의 연구가 아직 실용화되지 않고 있다 본 연구는 이에 대한 기초연구로서 돼지의 X 정자와 Y정자를 분리하기 위하여 불연속 percoll농도구배를 제조한 후 상층에 정액을 분주하여 120